基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异

doi:10.16431/j.cnki.1671-7236.2017.07.002

Abstract

Abstract:

In order to find out signaling pathways that related to the differences in temperature adaptability between buffaloes and Yellow cattle, RNA-sequencing and bioinformatics analysis were performed on the skin tissues of 3 adult buffaloes and 3 adult Yellow cattle. The results showed that 59 369 167 and 69 837 009 reads were obtained from buffaloes and Yellow cattle skin, respectively. The percentages of the uniquely mapped reads in buffaloes and Yellow cattle were 70.76% and 75.55%, respectively. There were 1 611 differentially expressed genes in buffaloes and Yellow cattle skin, 801 of which were up-regulated in buffaloes skin and 810 were down-regulated in buffaloes skin. GO analysis showed that there were 316, 263 and 278 differentially expressed genes classified to the cellular components, molecular functions and biological processes, respectively. KEGG analysis showed that the differentially expressed genes were enriched in 33 signaling pathways, some of which such as neuroactive ligand-receptor interaction, oxidative phosphorylation, cholinergic synapse, serotonergic synapse, GABAergic synapse and calcium signaling pathway might related to the differences in temperature adaptability between buffaloes and Yellow cattle. In summary, the synapse and oxidative phosphorylation might be related to the differences in temperature adaptability between buffalo and Yellow cattle. This study would lay a foundation for further understanding the molecular mechanism of temperature adaptation differences between buffaloes and Yellow cattle and molecular breeding.

Key words: transcriptome; signaling pathway; differentially expressed gene; skin; buffalo; Yellow cattle

CLC Number:

QI Yu, XING Yan-ping, PAN Jing, LING Yu, CAO Yu, MENG Fan-hua, XING Wen-kui. Analysis of the Differences in Temperature Adaptability Between Buffaloes and Yellow Cattle Based on Transcriptome Data of Skin Tissues[J]. , 2017, 44(7): 1906-1914.

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Analysis of the Differences in Temperature Adaptability Between Buffaloes and Yellow Cattle Based on Transcriptome Data of Skin Tissues

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