水牛瘦素受体基因密码子使用偏好性分析

doi:10.16431/j.cnki.1671-7236.2017.04.019

Abstract

Abstract:

The experiment was aimed to study the synonymous codon usage features of leptin receptor (LEPR) gene in buffalos, the codon usage differences and evolutionary relationships between buffalo and other reference species. The coding region of LEPR gene from 27 river buffalo and 41 swamp buffalo were sequencing,and Bos taurus,Bos mutus,Bos bison,Ovis aries,Capra hircus,Mus musculus and Homo sapiens were chosen as reference species from NCBI database. The results showed that all synonymous codons were used in the LEPR gene of river buffalo and swamp buffalo. Two kinds of buffalo had 28 biased usage codons. Among them,strongly biased codon was AGA (RSCU≥2),which indicated that codon usage features were similar in LEPR gene of two types of buffalo. Furthermore,buffalo and the species compared in this study were bias toward the synonymous codons with A/U at the third codon position in LEPR gene.However,the types and numbers of bias usage codons were different between buffalo and the species compared. Clustering analysis showed that the relationship between river buffalo and swamp buffalo was the closest,and they gathered into one group firstly,then they gathered with Bos taurus,Bos mutus and Bos bison,and further gathered in turn with Ovis aries,Capra hircus,Mus musculus and Homo sapiens.The frequency differences of synonymous codon usage between buffalo LEPR gene and yeast genome were less than that between buffalo and E.coli,Mus musculus genome,suggesting that yeast might be more suitable as an external expression system for buffalo LEPR gene.

Key words: river buffalo; swamp buffalo; LEPR gene; codon usage bias

CLC Number:

FAN Xin-yang, OUYANG Yi-na, WANG Peng-wu, ZHANG Yong-yun, TENG Xiao-hong, MIAO Yong-wang. Analysis of Codon Usage Bias of Leptin Receptor Gene in Buffalo[J]. , 2017, 44(4): 1069-1078.

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Analysis of Codon Usage Bias of Leptin Receptor Gene in Buffalo

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