猪FUT2基因密码子使用偏好性分析及优化

doi:10.16431/j.cnki.1671-7236.2018.07.017

Abstract

Abstract:

To reveal the codon usage pattern of porcine α-(1,2) fucosyltransferase 2 (FUT2) gene and increase its expression level in exogenous host,codon usage bias parameters of porcine FUT2 gene was analyzed using CodonW and EMBOSS Explorer,and then compared it with Drosophila, yeast and Escherichia coli genomes.Finally,according to the most closely codon usage pattern of model organism,the porcine FUT2 gene codon was optimized using JCat and OPTIMIZER.The results showed the expression level of porcine FUT2 gene was low,and it had 24 biased codons,23 of which biased to end with G/C at the third position.The differences in codon usage frequency between porcine FUT2 gene and Drosophila genome were less than those in Escherichia coli and yeast genomes,which proved Drosophila was more suitable for expressing the porcine FUT2 gene than Escherichia coli and yeast.The Drosophila genome was employed to optimize porcine FUT2 gene.The codon adaptation index of optimized porcine FUT2 gene was significantly improved,while the GC content of its CDS sequence was not obviously changed,indicating that the porcine FUT2 gene was successfully optimized in theory.This research revealed the codon usage pattern of porcine FUT2 gene from the translation level,and provided theoretical basis for selecting the best exogenous expression system and improving the expression level of the porcine FUT2 gene in host cells.

Key words: porcine; FUT2 gene; codon usage bias; codon optimization

CLC Number:

S813.3

DAI Kaiyu, HUANG Yanjie, WU Lisi, BAO Wenbin, WU Shenglong. Analysis and Optimization of Codon Usage Bias of Porcine FUT2 Gene[J]. , 2018, 45(7): 1863-1872.

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Analysis and Optimization of Codon Usage Bias of Porcine FUT2 Gene

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