Abstract: To establish a more exact and sensitive method to detect Eperythrozoon suis, two pairs of nested PCR primer were designed according to Eperythrozoon suis DNA sequence (AJ504999)and nested PCR assay was established. The optimum reaction conditions were screened, and sensitive, special and clinical samples tests were carried out. The results showed that Eperythrozoon suis genome DNA could be amplified by nested PCR,and Toxoplasma gondii, Streptococcus, Theileria sergenti and Eperythrozoon wenyonii genomic DNA could not. The minimum amount of DNA to detect was 0.124 fg/μL. After the detection of 55 clinical samples, results indicated that the positive detection rate of nested PCR was 23.7% higher than conventional PCR. This research offers a more exact and sensitive detection method and it is good value for application.

Key words: Eperythrozoon suis; nested PCR; establishment; application