›› 2011, Vol. 38 ›› Issue (1): 198-200.
• 疾病防治 • Previous Articles Next Articles
HE Ling,PEI Zhang-fu
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Abstract: Based on the variant sequences of PRRSV JXA1 strain and the genetic markers of JXA1-R strain,two pairs of primers were designed and synthesized,and RT-PCR methods were established for detecting the two viruses with specific gene fragments of 400 bp and 290 bp.There was no cross-reactions with those clinically relevant pathogens,such as classical swine fever virus, porcine pseudorabies virus, porcine circovirus type 2, porcine parvovirus. The sensitivity tests showed that the assay could detect 103 copies/μL RNA of JXA1 and 104 copies/μL RNA of JXA1-R.The RT-PCR established in this study, with high specificity and sensitivity,is very suit for clinic-sample detection.
Key words: PRRSV variant strain; JXA1-R; reverse transcriptase polymerase chain reaction(RT-PCR)
CLC Number:
S852.65+9.6
HE Ling;PEI Zhang-fu. Establishment of Distinguishing Method on Detecting Porcine Reproductive and Respiratory Syndrome Virus JXA1 and JXA1-R Strain[J]. , 2011, 38(1): 198-200.
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https://www.chvm.net/EN/Y2011/V38/I1/198