›› 2011, Vol. 38 ›› Issue (1): 198-200.

• 疾病防治 • Previous Articles     Next Articles

Establishment of Distinguishing Method on Detecting Porcine Reproductive and Respiratory Syndrome Virus JXA1 and JXA1-R Strain

HE Ling,PEI Zhang-fu   

  1. (Guangdong Dahuanong Animal Health Products Co.Ltd.,Xinxing 527400, China)
  • Received:1900-01-01 Revised:2010-11-07 Online:2011-01-01 Published:2011-01-01

Abstract: Based on the variant sequences of PRRSV JXA1 strain and the genetic markers of JXA1-R strain,two pairs of primers were designed and synthesized,and RT-PCR methods were established for detecting the two viruses with specific gene fragments of 400 bp and 290 bp.There was no cross-reactions with those clinically relevant pathogens,such as classical swine fever virus, porcine pseudorabies virus, porcine circovirus type 2, porcine parvovirus. The sensitivity tests showed that the assay could detect 103 copies/μL RNA of JXA1 and 104 copies/μL RNA of JXA1-R.The RT-PCR established in this study, with high specificity and sensitivity,is very suit for clinic-sample detection.

Key words: PRRSV variant strain; JXA1-R; reverse transcriptase polymerase chain reaction(RT-PCR)

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