贵州地区牛源金黄色葡萄球菌分离株16S rRNA分子鉴定及RAPD分型研究

›› 2011, Vol. 38 ›› Issue (1): 112-115.

• 生物技术 • Previous Articles Next Articles

Research of Identification by 16S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou

DENG Hai-ping，PU Wan-xia，LIANG Jian-ping,NI Chun-xia，MENG Xiao-qin

(Key Laboratory of New Animal Drug of Gansu Province,Key Laboratory of New Animal Drug,Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics，Chinese Academy of Agricultural Sciences，Lanzhou 730050，China)

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
Contact: PU Wan-xia

Abstract

Abstract: The 16S rRNA conserved sequence was used for isolation and identification of Staphylococcus aureus(S.aureus) strains causing bovine mastitis in Guizhou，and system of randomly amplified polymorphic DNA (RAPD) was established for research on genetic polymorphism of the S.aureus strains. The results showed that 37 S.aureus strains were isolated and identified from 69 mastitis milk samples. The 37 S.aureus strains amplified out 1 to 7 electrophoresis strips with sizes ranging from 400 to 4500 bp by RAPD. The strain were separated to 7 genotypes according to the fingerprint, 7 strain were type Ⅰ(18.9%)，2 strains were type Ⅱ(5.4%)，2 strains were type Ⅲ(5.4%)，1 strains was type Ⅳ(2.7%)，19 strains were type Ⅴ(51.4%)，1 strain was type Ⅵ(2.7%) and 5 strains were typeⅦ(13.5%). The preponderant S.aureus strains in this area was typeⅤ. Effects of geography and climate on transmission of pathogenic bacteria was the mainly reason of discrepancy on proportion of different genotype strains.

Key words: bovine mastitis; Staphylococcus aureus; 16S rRNA; RAPD; genotype〖

CLC Number:

S855.1

DENG Hai-ping;PU Wan-xia;LIANG Jian-ping;NI Chun-xia;MENG Xiao-qin. Research of Identification by 16S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou[J]. , 2011, 38(1): 112-115.

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Research of Identification by 16S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou

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