›› 2011, Vol. 38 ›› Issue (1): 116-118.

• 生物技术 • Previous Articles     Next Articles

Preliminary Establishment of Indirect ELISA Based on the VP3 Structural Protein of IBDV

SONG Tie-bin, CHEN Guang-dong   

  1. (Chaoyang Entry-Exit Inspection and Quarantine Bureau, Chaoyang 122000, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01

Abstract: In this study, VP3 gene was cloned and expressed from IBDV field isolate, which was isolated from a poultry farm in Hebei province. After purification using affinity chromatography, the fusion protein was used as a coating antigen for the preliminary establishment of an indirect ELISA for IBDV antibody detection.

Key words: infectious bursal disease virus; VP3 protein; indirect ELISA

CLC Number: