不同日龄白羽王鸽胸肌中lncRNA和mRNA功能分析

doi:10.16431/j.cnki.1671-7236.2024.05.004

Abstract

Abstract: 【Objective】 Differential lncRNA and mRNA of pectoralis muscle development in pigeons of different day-olds were analyzed to identify the key candidate genes affecting the growth and development of pigeons,explore the molecular mechanism of growth and development of pigeons,and provide a theoretical basis for the research on the promotion of the growth and development of pigeons.【Method】 White King pigeons were weighed at 1 (D1),7 (D7),14 (D14) and 25 (D25) days of age,followed by slaughtering at D7 and D25.lncRNAs from the pectoralis muscles of the selected day-old White King pigeons were sequenced to predict the target genes using RNA-Seq technology,construct the lncRNA-mRNA interactions network,and the screened differentially expressed lncRNAs were analyzed by GO and KEGG enrichment,and the reliability of sequencing results was verified by Real-time quantitative PCR.【Result】 Pigeons gained the fastest daily weight at the stage from 7 to 14 days of age,and the weight of breast pigeons reached the market age at 25 days of age.A total of 56 421 lncRNAs and 30 208 mRNAs were obtained from the six libraries constructed,of which 2 335 lncRNAs were expressed differentially and 1 553 mRNAs were targeted in the two groups.The lncRNA-mRNA regulatory network showed that there was mutual regulation between lncRNA and mRNA.The results of KEGG and GO analyses showed that the differential genes were associated with GTpase activity,etc.The differential lncRNAs and their target genes were mainly enriched in signaling pathways such as MAPK signaling pathway,skeletal muscle tissue development,TGF-β signaling pathway,Notch signaling pathway and other signaling pathways,and the key lncRNAs might regulate the pectoralis muscle development of the breast muscle of the pigeon through these signaling pathways.Among them,LTCONS_00012834,LTCONS_00032767,LTCONS_00045211,and LTCONS_00002003 targeted XIRP1,PITX3,BMPR1B,and MYLK4,respectively,and played key roles.Real-time quantitative PCR results were consistent with the high-throughput sequencing results,indicating the reliability of the sequencing data.【Conclusion】 A total of 2 335 differentially expressed lncRNAs and 1 553 mRNAs were obtained in this study,and four differentially expressed lncRNAs:LTCONS_00032767,LTCONS_00012834,LTCONS_00002003,and LTCONS_00045211 targeted genes related to skeletal muscle development PITX3,XIRP1,MYLK4,and BMPR1B,respectively,and these genes were regulated by multiple lncRNAs.The theoretical basis was provided for elucidating the molecular mechanism of pectoralis muscle growth and development in pigeons as well as the molecular selection and breeding of White King pigeons.

Key words: White King pigeons; pectoralis muscle; lncRNA; mRNA

CLC Number:

S836

ZHANG Chi, LIU Chao, WANG Yiman, MIAO Dongzhi, CHEN Jing, WANG Ying, YANG Haiming, WANG Zhiyue. Functional Analysis of lncRNA and mRNA in the Pectoralis Muscle of White King Pigeons at Different Days of Age[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1827-1836.

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Functional Analysis of lncRNA and mRNA in the Pectoralis Muscle of White King Pigeons at Different Days of Age

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