China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (2): 453-461.doi: 10.16431/j.cnki.1671-7236.2024.02.002

• Biotechnology • Previous Articles     Next Articles

Effect of Long Non-coding RNA MSTRG.14200 on Porcine Skeletal Muscle Satellite Cell Differentiation and Muscle Fiber Transformation

YANG Yuming1, ZHAO Xinming1, TAN Baohua1, XIAO Liyao1, LU Geyan1, ZHAI Lijun1, HUANG Yiyang1, HONG Linjun1,2, GU Ting1,2   

  1. 1. National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
    2. Key Laboratory of South China Modern Biological Seed Industry, Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China
  • Received:2023-08-18 Online:2024-02-05 Published:2024-01-29
  • Contact: 广东省重点研发计划项目(2022B0202090002)
  • Supported by:
    The project was supported by the National Key Research and Development Program of China (2019YFC1905301);National Natural Science Foundation of China (22078115,21776108,21690083,22008078).

Abstract: 【Objective】 This experiment was conducted to investigate the effects of long non-coding RNA (lncRNA) MSTRG.14200 on myogenic differentiation of porcine skeletal muscle satellite cells and the transformation of different types of muscle fibers.【Method】 Three 6-month-old Duroc pigs were slaughtered, and the heart, liver, spleen, lung, kidney, stomach, longissimus dorsi muscle, leg muscle, soleus and extensor digitorum longus were collected.Real-time quantitative PCR was used to detect the expression of MSTRG.14200 in different tissues.The MSTRG.14200 overexpression vector pcDNA3.1-14200 was constructed and transfected into PSCs cells, and the cells were collected after 3 days of induced differentiation.The mRNA and protein expressions of myosin heavy chain (MyHC), myogenin (MyoG), mydifferentiation factor (MyoD), MyHCⅠ, MyHCⅡa, MyHCⅡb and MyHCⅡx were detected by Real-time quantitative PCR and Western blotting, respectively.The percentage of MyHC, MyHCⅠ and MyHCⅡb positive cells was detected by immunofluorescence assay.【Result】 MSTRG.14200 was expressed in different tissues of Duroc pigs, and the expression was the highest in longissimus dorsi muscle, which was extremely significantly higher than other tissues (P<0.01), and there were significant differences in the expression of extensor digitorum longus and soleus (P<0.05).After overexpression of MSTRG.14200, mRNA levels of MyoD and MyHC genes related to cell differentiation were extremely significantly increased (P<0.01), and protein levels of MyoD, MyoG and MyHC were significantly or extremely significantly increased (P<0.05 or P<0.01), the percentage of MyHC positive cells was extremely significantly increased (P<0.01).The mRNA and protein expressions of MyHCⅠ gene related to muscle fiber transformation and the proportion of positive cells were significantly or extremely significantly decreased (P<0.05 or P<0.01), the mRNA and protein expression of MyHCb gene and the percentage of positive cells were significantly increased (P<0.05).【Conclusion】 MSTRG.14200 could promote myogenic differentiation of porcine skeletal muscle and promote the transformation of slow muscle fibers into fast muscle fibers.The results provided a theoretical basis for exploring the molecular mechanism of transformation of porcine skeletal muscle fiber type.

Key words: pigs; long non-coding RNA (lncRNA); MSTRG.14200; myogenic differentiation; muscle fiber transformation

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