异亮氨酸的营养生理作用及其在猪生产中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2023.12.011

Abstract

Abstract: Isoleucine,as one of the important essential amino acids for pigs,is a member of the branched chain amino acid.Isoleucine has the physiological functions of maintaining glucose homeostasis,regulating protein and lipid synthesis and catabolism.Dietary isoleucine at optimal level can promote the growth performance,improve the intestinal morphology and structure,promote the intestinal development of pigs by promoting the expression of intestinal nutrient transporters and regulating intestinal flora,improve immunity by promoting the proliferation of immune cells,increasing the expression of immune reactive substances and host defense peptides.It can also improve the carcass traits and pork quality.Under the background of high feed price and active promotion of diversified diets plan with lower protein and lower soybean meal,the application of isoleucine in pig nutrition has attracted much attention,the biosynthesis technology of L-isoleucine and balance of isoleucine and other amino acids especially branched chain amino acid in lower protein and lower soybean meal diets.In addition,the nutritional requirements of pigs for isoleucine in different physiological stages,and the ileal digestibable isoleucine parameters of common feed materials and unconventional feed materials and their dynamic prediction models are worthy of further study in future production and application.Thus,in this paper,the nutritional physiological effects of isoleucine and its nutritional requirements and application in pig production were reviewed,in order to provide reference for precise nutrition supply of pigs and cost reduction and efficiency increase in pig breeding in the future.

Key words: isoleucine; pigs; growth performance; gut health; immune function

CLC Number:

S815.1

LU Qi, YANG Xuefen, WANG Li, ZHU Cui. Research Progress on Nutritional Physiological Effects of Isoleucine and Its Application in Pig Production[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4886-4898.

References

[1] 孔帅,方应浩,周航,等.微生物发酵法生产L-异亮氨酸的研究进展[J].中国酿造,2020,39(9):30-36. KONG S,FANG Y H,ZHOU H,et al.Research progress of L-isoleucine production by microbial fermentation[J].China Brewing,2020,39(9):30-36.(in Chinese)
[2] 史仍飞,袁海平.支链氨基酸代谢与运动[J].上海体育学院学报,2003,5:65-67. SHI R F,YUAN H P.Branched chain amino acid metabolism and motility[J].Journal of Shanghai Physical Education Institute,2003,5:65-67.(in Chinese)
[3] ZHAO Y,TIAN G,CHEN D,et al.Effect of different dietary protein levels and amino acids supplementation patterns on growth performance,carcass characteristics and nitrogen excretion in growing-finishing pigs[J].Journal of Animal Science and Biotechnology,2019,10:75
[4] FIGUEROA J L,LEWIS A J,MILLER P S,et al.Growth,carcass traits,and plasma amino acid concentrations of gilts fed low-protein diets supplemented with amino acids including histidine,isoleucine,and valine[J].American Society of Animal Science,2003,81(6):1529-1537.
[5] 王莉苹.牙鲆(Paralichthys olivaceus)饲料中亮氨酸与异亮氨酸交互作用的研究[D].大连:大连海洋大学,2017. WANG L P.Study of interactive effect of dietary leucine and isoleucine on Japanese flounder Paralichthys olivaceus[D].Dalian:Dalian Ocean University,2017.(in Chinese)
[6] 国家药典委员会.中华人民共和国药典二部(2020年版)[M].北京:中国医药科技出版社,2020. CHINESE PHARMACOPEIA COMMISSION.Pharmacopoeia of the People's Republic of China VolumeⅡ(2020 Edition)[M].Beijing:China Medical Science Press,2020.(in Chinese)
[7] HERMAN M A,SHE P,PERONI O D,et al.Adipose tissue branched chain amino acid (BCAA) metabolism modulates circulating BCAA levels[J].Journal of Biological Chemistry,2010,285(15):11348-11356.
[8] BROSNAN J T,BROSNAN M E.Branched-chain amino acids:Enzyme and substrate regulation[J].The Journal of Nutrition,2006,136(1 Suppl):207S-211S.
[9] SHE P,ZHOU Y,ZHANG Z,et al.Disruption of BCAA metabolism in mice impairs exercise metabolism and endurance[J].Journal of Applied Physiology,2010,108(4):941-949.
[10] SURYAWAN A,HAWES J W,HARRIS R A,et al.A molecular model of human branched-chain amino acid metabolism[J]. The American Journal of Clinical Nutrition,1998,68(1):72-81.
[11] LI P,KNABE D A,KIM S W,et al.Lactating porcine mammary tissue catabolizes branched-chain amino acids for glutamine and aspartate synthesis[J].The Journal of Nutrition,2009,139(8):1502-1509.
[12] LEI J,FENG D,ZHANG Y,et al.Regulation of leucine catabolism by metabolic fuels in mammary epithelial cells[J].Amino Acids,2012,43(5):2179-2189.
[13] PENG H,WANG Y,LUO W.Multifaceted role of branched-chain amino acid metabolism in cancer[J].Oncogene,2020,39(44):6747-6756.
[14] ADEVA-ANDANY M M,LÓPEZ-MASIDE L,DONAPETRY-GARCÍA C,et al.Enzymes involved in branched-chain amino acid metabolism in humans[J].Amino Acids,2017,49(6):1005-1028.
[15] YUDKOFF M.Interactions in the metabolism of glutamate and the branched-chain amino acids and ketoacids in the CNS[J].Neurochemical Research,2017,42(1):10-18.
[16] 陈力,柴捷,张廷焕,等.谷氨酸的生物学功能及其在猪生产应用中的研究进展[J].中国畜牧杂志,2023,59(8),36-40. CHEN L,CHAI J,ZHANG T H,et al.The research progress on the biological function of glutamic acid and its application in pig production[J].Chinese Journal of Animal Science,2023,59(8),36-40.(in Chinese)
[17] YONESHIRO T,WANG Q,TAJIMA K,et al.BCAA catabolism in brown fat controls energy homeostasis through SLC25A44[J]. Nature,2019,7771(572):614-619.
[18] MANOLI I,VENDITTI C P.Disorders of branched chain amino acid metabolism[J].Translational Science of Rare Diseases,2016,1(2):91-110.
[19] 董晓慧.支链氨基酸营养作用与拮抗[J].饲料博览,1999,11(10):17-18. DONG X H.Branched-chain amino acid nutrition and antagonism[J].Feed Review,1999,11(10):17-18.(in Chinese)
[20] ZHANG S,YANG Q,REN M,et al.Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na⁺/glucose co-transporter 1(SGLT-1) and GLUT2[J].British Journal of Nutrition,2016,116(4):593-602.
[21] DOI M,YAMAOKA I,FUKUNAGA T,et al.Isoleucine,a potent plasma glucose-lowering amino acid,stimulates glucose uptake in C2C12 myotubes[J].Biochemical and Biophysical Research Communications,2003,312(4):1111-1117.
[22] ZHANG S,ZENG X,REN M,et al.Novel metabolic and physiological functions of branched chain amino acids:A review[J]. Journal of Animal Science and Biotechnology,2017,8:10.
[23] GOODARZI P,HABIBI M,GORTON M W,et al.Dietary isoleucine and valine:Effects on lipid metabolism and ureagenesis in pigs fed with protein restricted diets[J].Metabolites,2023,13:89.
[24] FAN L,HSIEH P N,SWEET D R,et al.Krüppel-like factor 15:Regulator of BCAA metabolism and circadian protein rhythmicity[J]. Pharmacology Research,2018,130:123-126.
[25] DOI M,YAMAOKA I,NAKAYAMA M,et al.Hypoglycemic effect of isoleucine involves increased muscle glucose uptake and whole body glucose oxidation and decreased hepatic gluconeogenesis[J].American Journal of Physiology-Endocrinology and Metabolism,2007,292(6):E1683-E1693.
[26] SHIMOMURA Y,MURAKAMI T,NAKAI N,et al.Exercise promotes BCAA catabolism:Effects of BCAA supplementation on skeletal muscle during exercise[J]. The Journal of Nutrition,2004,134(6 Suppl):1583S-1587S.
[27] SHE P,REID T M,BRONSON S K,et al.Disruption of BCATm in mice leads to increased energy expenditure associated with the activation of a futile protein turnover cycle[J].Cell Metabolism,2007,6(3):181-194.
[28] CROWE M J,WEATHERSON J N,BOWDEN B F.Effects of dietary leucine supplementation on exercise performance[J].European Journal of Applied Physiology,2006,97(6):664-672.
[29] NISHIMURA J,MASAKI T,ARAKAWA M,et al.Isoleucine prevents the accumulation of tissue triglycerides and upregulates the expression of PPARα and uncoupling protein in diet-induced obese mice[J].The Journal of Nutrition,2010,140(3):496-500.
[30] DU Y,MENG Q,ZHANG Q,et al.Isoleucine or valine deprivation stimulates fat loss via increasing energy expenditure and regulating lipid metabolism in WAT[J].Amino Acids,2012,43(2):725-734.
[31] 廖国周,柴仕名.支链氨基酸营养研究进展[J].饲料博览,2005,4:7-10. LIAO G Z,CHAI S M.Advances in branched-chain amino acid nutrition[J].Feed Review,2005,4:7-10.(in Chinese)
[32] WU G.Functional amino acids in nutrition and health[J]. Amino Acids,2013,45(3):407-411.
[33] LIU K,SHEN J,CAO Y,et al.Duodenal infusions of isoleucine influence pancreatic exocrine function in dairy heifers[J]. Archives of Animal Nutrition,2018,72(1):31-41.
[34] APPUHAMY J A,KNOEBEL N A,NAYANANJALIE W A,et al.Isoleucine and leucine independently regulate mTOR signaling and protein synthesis in MAC-T cells and bovine mammary tissue slices[J].The Journal of Nutrition,2012,142(3):484-491.
[35] YODER P S,HUANG X,TEIXEIRA I A,et al.Effects of jugular infused methionine,lysine,and histidine as a group or leucine and isoleucine as a group on production and metabolism in lactating dairy cows[J].Journal of Dairy Science,2020,103(3):2387-2404.
[36] ZHAO J,LIU Y,JIANG J,et al.Effects of dietary isoleucine on growth,the digestion and absorption capacity and gene expression in hepatopancreas and intestine of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture,2012,368-369:117-128.
[37] 张世海.支链氨基酸调节仔猪肠道和肌肉中氨基酸及葡萄糖转运的研究[D].北京:中国农业大学,2016. ZHANG S H.Branched-chain amino acids modulate amino acid and glucose transport in intestine and muscle of piglets[D].Beijing:China Agricultural University,2016.(in Chinese)
[38] LORDELO M M,GASPAR A M,LE BELLEGO L,et al.Isoleucine and valine supplementation of a low-protein corn-wheat-soybean meal-based diet for piglets:Growth performance and nitrogen balance[J].Journal of Animal Science,2008,86(11):2936-2941.
[39] VAN MILGEN J,GLOAGUEN M,LE FLOC'H N,et al.Meta-analysis of the response of growing pigs to the isoleucine concentration in the diet[J].Animal,2012,6(10):1601-1608.
[40] LUO Y,ZHANG X,ZHU Z,et al.Surplus dietary isoleucine intake enhanced monounsaturated fatty acid synthesis and fat accumulation in skeletal muscle of finishing pigs[J]. Journal of Animal Science and Biotechnology,2018,9:88.
[41] 罗燕红,张鑫,覃春富,等.饲粮异亮氨酸水平对肥育猪生长性能、胴体性状和肉品质的影响[J].动物营养学报,2017,29(6):1884-1894. LUO Y H,ZHANG X,QIN C F,et al.Effects of dietary isoleucine level on growth performance,carcass traits and meat quality of finishing pigs[J].Chinese Journal of Animal Nutrition,2017,29(6):1884-1894.(in Chinese).
[42] CERVANTES-RAMÍREZ M,MENDEZ-TRUJILLO V,ARAIZA-PIÑA B A,et al.Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin,serum concentration of amino acids,and growth performance of pigs[J].Genetics and Molecular Research,2013,12(1):115-126.
[43] HABIBI M,GOODARZI P,SHILI C N,et al.A mixture of valine and isoleucine restores the growth of protein-restricted pigs likely through improved gut development,hepatic IGF-1 pathway,and plasma metabolomic profile[J]. International Journal of Molecular Sciences,2022,23(6):3300.
[44] GOODARZI P,WILEMAN C M,HABIBI M,et al.Effect of isoleucine and added valine on performance,nutrients digestibility and gut microbiota composition of pigs fed with very low protein diets[J].International Journal of Molecular Sciences,2022,23(23):14886.
[45] GLOAGUEN M,LE FLOC'H N,BROSSARD L,et al.Response of piglets to the valine content in diet in combination with the supply of other branched-chain amino acids[J].Animal,2011,5(11):1734-1742.
[46] CLARK A B,TOKACH M D,DEROUCHEY J M,et al.Modeling the effects of standardized ileal digestible isoleucine to lysine ratio on growth performance of nursery pigs[J].Translational Animal Science,2017,1(4):437-447.
[47] ZHANG S,QIAO S,REN M,et al.Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs[J].Amino Acids,2013,45(5):1191-1205.
[48] TIAN M,HENG J,SONG H,et al.Branched chain amino acids stimulate gut satiety hormone cholecystokinin secretion through activation of the umami taste receptor T1R1/T1R3 using an in vitro porcine jejunum model[J]. Food&Function,2019,10(6):3356-3367.
[49] TIAN M,HENG J,SONG H,et al.Dietary branched-chain amino acids regulate food intake partly through intestinal and hypothalamic amino acid receptors in piglets[J].Journal of Agricultural and Food Chemistry,2019,67(24):6809-6818.
[50] WU G.Intestinal mucosal amino acid catabolism[J]. The Journal of Nutrition,1998,128(8):1249-1252.
[51] WU G.Amino acids:Metabolism,functions,and nutrition[J].Amino Acids,2009,37(1):1-17.
[52] REN M,ZHANG S H,ZENG X F,et al.Branched-chain amino acids are beneficial to maintain growth performance and intestinal immune-related function in weaned piglets fed protein restricted diet[J].Asian-Australasian Journal of Animal Sciences,2015,28(12):1742-1750.
[53] MAO X,SUN R,WANG Q,et al.L-isoleucine administration alleviates DSS-induced colitis by regulating TLR4/MyD88/NF-κB pathway in rats[J].Frontiers in Immunology,2022,12:817583.
[54] 任曼.支链氨基酸调控仔猪肠道防御素表达和免疫屏障功能的研究[D].北京:中国农业大学,2014. REN M.Branched-chain amino acids modulate intestinal defensin expression and immune barrier function of piglets[D].Beijing:China Agricultural University,2014.(in Chinese)
[55] 毛湘冰,古长松,陈代文,等.饲粮添加异亮氨酸对轮状病毒攻毒断奶仔猪回肠屏障功能的影响[J].动物营养学报,2019,31(12):5493-5499. MAO X B,GU C S,CHEN D W,et al.Effects of dietary isoleucine on ileal barrier function of weaned piglets challenged by Rotavirus[J].Chinese Journal of Animal Nutrition,2019,31(12):5493-5499.(in Chinese)
[56] ZHAO J,FENG L,LIU Y,et al.Effect of dietary isoleucine on the immunity,antioxidant status,tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Fish&Shellfish Immunology,2014,41(2):663-673.
[57] DAI Z,ZHANG J,WU G,et al.Utilization of amino acids by bacteria from the pig small intestine[J].Amino Acids,2010,39(5):1201-1215.
[58] SMITH E A,MACFARLANE G T.Dissimilatory amino acid metabolism in human colonic bacteria[J].Anaerobe,1997,3(5):327-337.
[59] 赵玉梅.低蛋白日粮必需氨基酸平衡模式对生长育肥猪生长性能、氮排放及后肠微生物的影响[D].雅安:四川农业大学,2018. ZHAO Y M.Effects of different essential amino acids balanced patterns in low-protein diets on growth performance,nitrogen excretion and gut microflora of growing-finishing pigs[D].Ya'an:Sichuan Agricultural University,2018.(in Chinese)
[60] GU C,MAO X,CHEN D,et al.Isoleucine plays an important role for maintaining immune function[J].Current Protein&Peptide Science,2019,20(7):644-651.
[61] HALE L L,PHARR G T,BURGESS S C,et al.Isoleucine needs of thirty-to forty-day-old female chickens:Immunity[J]. Poultry Science,2004,83(12):1979-1985.
[62] MAO X,GU C,REN M,et al.L-isoleucine administration alleviates Rotavirus infection and immune response in the weaned piglet model[J]. Frontiers in Immunology,2018,9:1654.
[63] ZHAO J,LIU Y,JIANG J,et al.Effects of dietary isoleucine on the immune response,antioxidant status and gene expression in the head kidney of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Fish&Shellfish Immunology,2013,35(2):572-580.
[64] REN M,LIU C,ZENG X,et al.Amino acids modulates the intestinal proteome associated with immune and stress response in weaning pig[J].Molecular Biology Reports,2014,41(6):3611-3620.
[65] REN M,ZHANG S,LIU X,et al.Different lipopolysaccharide branched-chain amino acids modulate porcine intestinal endogenous β-defensin expression through the Sirt1/ERK/90RSK pathway[J].Journal of Agricultural and Food Chemistry,2016,64(17):3371-3379.
[66] MAO X,QI S,YU B,et al.Zn²⁺ and L-isoleucine induce the expressions of porcine β-defensins in IPEC-J2 cells[J].Molecular Biology Reports,2013,40(2):1547-1552.
[67] MA W,MAO P,GUO L,et al.Crystalline amino acids supplementation improves the performance and carcass traits in late-finishing gilts fed low-protein diets[J].Animal Science Journal,2020,91(1):e13317.
[68] XU D,WANG Y,ZHANG X,et al.Dietary valine/isoleucine ratio impact carcass characteristics,meat edible quality and nutritional values in finishing crossbred Duroc×Landrace×Yorkshire pigs with different slaughter weights[J].Frontiers in Nutrition,2022,11:9899871.
[69] WOOD J D,ENSER M,FISHER A V,et al.Fat deposition,fatty acid composition and meat quality:A review[J]. Meat Science,2008,78(4):343-358.
[70] XU D,WANG Y,JIAO N,et al.The coordination of dietary valine and isoleucine on water holding capacity,pH value and protein solubility of fresh meat in finishing pigs[J].Meat Science,2020,163(5):108074.
[71] 张相鑫,王晶晶,BART M,等.异亮氨酸是仔猪安全断奶中氨基酸平衡的关键环节[J].国外畜牧学(猪与禽),2022,42(5):19-22. ZHANG X X,WANG J J,BART M,et al.Isoleucine:A missing link in the pivotal role of amino acids in safe weaning[J].Animal Science Abroad (Pigs and Poultry),2022,42(5):19-22.(in Chinese)
[72] 杨静,谢高妙,李结,等.不同低蛋白质水平下猪异亮氨酸需要量的评估[J].广东饲料,2022,31(12):31-34. YANG J,XIE G M,LI J,et al.Evaluation of isoleucine requirements in pigs at different low protein levels[J].Guangdong Feed,2022,31(12):31-34.(in Chinese)
[73] NORGAARD J V,SHRESTHA A,KROGH U,et al.Isoleucine requirement of pigs weighing 8 to 18 kg fed blood cell-free diets[J]. Journal of Animal Science,2013,91(8):3759-3765.
[74] 周相超,杨凤娟,霍应峰,等.低氮日粮条件下生长猪标准回肠可消化异亮氨酸需要量研究[J].中国畜牧杂志,2018,54(5):67-73. ZHOU X C,YANG F J,HUO Y F,et al.Study on requirement of standardized ileal digestible isoleucine for growing pigs fed low crude protein diets[J].Chinese Journal of Animal Science,2018,54(5):67-73.(in Chinese)
[75] FRANCO D J,JOSEPHSON J K,MOEHN S,et al.Isoleucine requirement of pregnant sows[J].Journal of Animal Science,2013,91(8):3859-3866.
[76] 龙运艳,邹知明,覃小荣,等.妊娠母猪氨基酸需要的研究进展[J].广西畜牧兽医,2016,32(4):185-187. LONG Y Y,ZOU Z M,QIN X R,et al.Advances in amino acid requirements of pregnant sows[J].Guangxi Journal of Animal Husbandry&Veterinary Medicine,2016,32(4):185-187.(in Chinese)
[77] KIM S W,HURLEY W L,WU G,et al.Ideal amino acid balance for sows during gestation and lactation[J].Journal of Animal Science,2009,87(14):E123-E132.
[78] WILTAFSKY M K,BARTELT J,RELANDEAU C,et al.Estimation of the optimum ratio of standardized ileal digestible isoleucine to lysine for eight-to twenty five-kilogram pigs in diets containing spray-dried blood cells or corn gluten feed as a protein source[J].Journal of Animal Science,2009,87(8):2554-2564.

Research Progress on Nutritional Physiological Effects of Isoleucine and Its Application in Pig Production

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