China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (2): 530-539.doi: 10.16431/j.cnki.1671-7236.2024.02.010

• Nutrition and Feed • Previous Articles     Next Articles

Effects of Whole-plant Corn Silage on the Gut Microbiota of Tibetan Pigs

WU Danning1, ZHANG Yanan1, HUANG Tao1, ZHOU Xiaolong1, WANG Han1, LI Xiangchen1, LI Hongjun2, WEN Jun2, ZHANG Qi3, ZHAO Ayong1, YANG Songbai1   

  1. 1. Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Lin'an 311300, China;
    2. Aba Bowen Agriculture and Animal Husbandry Technology Co., Ltd., Jiuzhaigou 623402, China;
    3. Jiuzhaigou County Bureau of Science, Technology, Agriculture and Animal Husbandry, Jiuzhaigou 624603, China
  • Received:2023-05-29 Online:2024-02-05 Published:2024-01-29
  • Contact: 浙江省重点研发计划项目(2021C04034)
  • Supported by:
    The project was supported by the National Key Research and Development Program of China (2019YFC1905301);National Natural Science Foundation of China (22078115,21776108,21690083,22008078).

Abstract: 【Objective】 This study aimed to explore the effects of adding whole-plant corn silage to the feed on the gut microbial community of Tibetan pigs.【Method】 A total of 40 Tibetan pigs with similar age and weight ((24.26±2.78) kg), half male and half female, were randomly divided into control and experimental groups, with 20 pigs in each group.The pigs in control group were fed basal diet, while the pigs in experimental group were fed the basal diet with 30% whole-plant corn silage replacing.Feeding time was 7 months.After the feeding period, 40 fecal samples were collected, 20 from each group.The 16S rRNA gene sequencing technology was used to analyze the effects of whole-plant corn silage on the composition and structure of the gut microbiota in Tibetan pigs.【Result】 The results showed that a total of 19 phyla, 33 classes, 67 orders, 123 families, 277 genera, and 524 species were identified in both groups.The dominant phyla were Firmicutes and Bacteroidota.The Shannon index of gut microbiota in the experimental group was significantly lower than that of the control group (P<0.05).A Wilcoxon rank-sum test was used to compare the abundance of microbial taxa between two groups.The experimental group showed a significant increase in the abundance of Spirochaetota, Fibrobacterota, Treponema, Lactobacillus, and Streptococcus, while Actinobacteriota, Proteobacteria, and Christensenellaceae_R-7_group were significantly decreased (P<0.05).Results of the LEfSe analysis showed that the abundance of Streptococcus alactolyticus and Lactobacillus amylovorus were significantly increased in the experimental group compare to control group (P<0.05).【Conclusion】 Feeding whole-plant corn silaga altered the structure of gut microbiota, increased the relative abundance of beneficial bacteria in Tibetan pigs, and might have a positive impact on gut health.

Key words: whole-plant corn silage; Tibetan pigs; gut microbiota

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