ADSL基因启动子区遗传变异与鸡肉冻藏新鲜度的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.11.015

Abstract

Abstract: This study was aimed to explore the genetic variations in promoter region of adenylosuccinate lyase (ADSL) and its correlation with chicken freshness after freezing.161 Partridge Shank chickens were employed,the genetic variation of promoter region of ADSL gene was scanned by pool-based sequencing to discover candidate SNP,and the candidate SNP was genotyped using allele specific PCR.The association analysis was performed between the candidate SNP and four indexes (meat color,body weight,freshness (K value) after freezing of 60 days and pH) in both genotypes.The results showed that one SNP (c.-1670 C>A) was identified at 1 670 bp upstream of ATG in the promoter region of ADSL gene.The K value of muscle freshness of AA genotype (23.61%) was extremely significantly lower than that of CA (33.00%) and CC (33.56%) genotypes (P<0.01),indicating that the muscle of AA genotype was fresher than that of CA and CC genotypes after freezing for 60 days.No significant difference in meat freshness was observed between CA and CC genotypes (P>0.05).There was no significant difference in the body weight,meat color and pH among three genotypes (P>0.05).Bioinformatics prediction results showed that c.-1670 C>A caused the transcription factor site to change in the promoter region of ADSL gene,which might affect the expression of ADSL gene.This study concluded that there was a significant correlation between c.-1670 C>A in the promoter region of ADSL gene and freshness K value of breast muscle in Partridge Shank chickens after freezing for 60 days,c.-1670 C>A could be used as a candidate molecular genetic marker for meat freshness in Partridge Shank chickens.

Key words: Partridge Shank chickens; ADSL gene; SNP; molecular marker

CLC Number:

S831.2

YU Shigang, SUN Xueliang, WANG Gang, LIAO Juan, HU Qiang. Association Analysis Between Genetic Variation in Promoter Region of ADSL Gene and Freshness of Frozen Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3536-3545.

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Association Analysis Between Genetic Variation in Promoter Region of ADSL Gene and Freshness of Frozen Chicken

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