盐津乌骨鸡MyoG和IGF-1基因表达及其与生长、屠宰性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.08.018

Abstract

Abstract: In order to study the effect of myogenin (MyoG) and insulin-like growth factor-1 (IGF-1) genes expression on the growth and development of poultry,Yanjin Black-bone and Daweishan Mini chickens with large differences in body size were chosen.At the age of 300 days,the body weight,body size and slaughter traits of 20 chickens were measured for comparative analysis,and the relative expression of MyoG and IGF-1 genes mRNA in chest muscle,leg muscle and liver were detected.Finally,the correlation between growth and slaughter traits and gene relative expression were analyzed,respectively.The results showed that the body weight,body size and slaughter traits of Yanjin Black-bone chickens were higher than that of Daweishan Mini chickens.Moreover,the expression of MyoG and IGF-1 genes in chest muscle,leg muscle and liver of Yanjin Black-bone chickens were also higher than that of Daweishan Mini chickens,and the expression trend was as follows:Chest muscle > leg muscle > liver.Correlation analysis results showed that the expression of MyoG and IGF-1 genes in pectoralis muscle,leg muscle and liver of Yanjin Black-bone and Daweishan Mini chickens were significantly positively correlated with body weight,body size and slaughter traits (P<0.05).The relative expression of MyoG gene was extremely significantly correlated with all the traits except chest depth (P<0.01),but the relative expression of IGF-1 gene was significantly correlated with body weight,body length,chest depth and sternum length (P<0.05).Therefore,MyoG and IGF-1 genes could be used as candidate genes for growth traits of Yanjin Black-bone and Daweishan Mini chickens for breeding.

Key words: Yanjin Black-bone chickens; Daweishan Mini chickens; IGF-1 gene; MyoG gene; growth trait; slaughter trait

CLC Number:

S813.3

LI Fengyun, ZHANG Ru, WANG Kun, DOU Tengfei, LIU Mingzheng, LI Qihua, YANG Hongtao, JIA Junjing, GE Changrong. Expression of MyoG and IGF-1 Genes and Its Correlation Analysis with Growth and Slaughter Traits of Yanjin Black-bone Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2502-2509.

References

[1] FENG X,WANG Z Y,WANG F,et al.Dual function of VGLL4 in muscle regeneration[J].EMBO Journal,2019,38(17):e101051.
[2] RAHAIE A,TOGHYANI M,EGHBALSAIED S.Cotreatment of IGF1 and fadrozole upregulates the expression of RSPO1,SOX9,and AMH in chicken embryos[J].Cells Tissues Organs,2018,206(4-5):218-228.
[3] 柴志欣,信金伟,王会,等. MyoG基因多态性与牦牛生长性能的关联分析[J].华北农学报,2018,33(6):108-115. CAI Z X,XIN J W,WANG H,et al.Polymorphisms of MyoG gene and analysis of relevance with growth performances in yak[J].Acta Agriculturae Boreali-Sinica,2018,33(6):108-115.(in Chinese)
[4] 豆腾飞,佟荟全,荣华,等.大围山微型鸡和艾维茵肉鸡骨骼肌卫星细胞增殖分化及Myf5基因表达差异研究[J].中国家禽,2017,39(11):8-12. DOU T F,TONG H Q,RONG H,et al.Study on differentiation and Myf5 gene expression in skeletal muscle satellite cells of Daweishan Mini chicken and Avian broiler[J].China Poultry,2017,39(11):8-12.(in Chinese)
[5] FRANCETIC T,LI Q.Skeletal myogenesis and Myf5 activation[J].Transcription,2011,2(3):109-114.
[6] MORESI V,WILLIAMS A H,MEADOWS E,et al.Myogenin and class Ⅱ HDACs control neurogenic muscle at rophy by inducing E3 ubiquitin ligases[J].Cell,2010,143(1):25-34.
[7] JIA H T,TSAV H J,SCHNUDT J.Analysis of binding and activating functions of the chick muscle acetylcholine receptor γ-subunit upscream sequence[J]Cellular and Molecular Neurobiology,1992,12:241-258.
[8] 王坤,豆腾飞,徐志强,等.大围山微型鸡和艾维茵肉鸡PFKM基因表达量与pH值及肌糖原含量相关性研究[J].中国家禽,2018,40(14):10-13. WANG K,DOU T F,XU Z Q,et al.Correlation analysis on PFKM gene expression,pH value,muscle glycogen content in Daweishan Mini chicken and Avian broiler[J].China Poultry,2018,40(14):10-13.(in Chinese)
[9] 林亚秋,张倡珲,郑玉才,等.九龙牦牛肌细胞生成素基因的克隆及其表达谱[J].畜牧兽医学报,2014,45(2):207-211. LIN Y Q,ZHANG C H,ZHENG Y C,et al.Cloning and expression profiling of myogenin gene of Jiulong yak[J].Chinese Journal of Animal and Veterinary Sciences,2014,45(2):207-211.(in Chinese)
[10] 李莉鑫,任华伟,李艺雷,等.不同发育时期小鼠心肌MyoG基因的表达分析[J].山西农业科学,2019,47(4):677-680. LI L X,REN H W,LI Y L,et al.MyoG expression analysis in the cardiac muscle at different developmental stages in mice[J].Journal of Shanxi Agricultural Sciences,2019,47(4):677-680.(in Chinese)
[11] ZHANG G X,TANG X,ZHANG T,et al.Expression profiles and association analysis with growth traits of the MyoG and Myf5 genes in the Jinghai Yellow chicken[J].Molecular Biology Reports,2014,41(11):7331-7338.
[12] AMILLS M,JIMEMEA N,VILLALBA D,et al.Identification of three single nucleotide polymorphisms in the chicken in-solution like growth factor 1 and 2 genes and their associations with growth and feeding traits[J].Poultry Science,2003,82(10):1486-1493.
[13] ALISON L,BRITTAIN,JOHN J,et al.A review of renal GH/IGF1 family gene expression in chronic kidney diseases[J].Growth Hormone & IGF Research,2019,48-49:1-4.
[14] 中国畜牧业协会.家禽生产性能名词术语和度量统计方法[A].中国畜牧业协会禽业分会.首届中国黄羽肉鸡行业发展大会会刊[C].2008. CHINA ANIMAL HUSBANDRY ASSOCIATION.Poultry production performance terms and measurement statistical methods[A].Poultry Branch of China Animal Husbandry Association.The first China Yellow Feather Broiler Industry Development Conference[C].2008.(in Chinese)
[15] 贾俊静,文生萍,熊保良,等.云南屏边大围山微型鸡遗传多样性[J].云南农业大学学报,2009,24(5):654-660. JIA J J,WEN S P,XIONG B L,et al.Genetic diversity of Daweishan Mini chickens based on microsatellite markers[J].Journal of Yunnan Agricultural University,2009,24(5):654-660.(in Chinese)
[16] HUGHES S M,SCHIAFFINO S.Control of muscle fiber size:Acrucial factor in ageing[J].Acta Physiologica Scandinavica,1999,167:307-312.
[17] SABOURIN L A,GIRGIS-GABARDO A,SCALE P,et a1.Reduced differ-entiation potential of primary MyoD^-/- myogenic cells derived from adult skeletal muscle[J].The Journal of Cell Biology,1999,144(4):631-643.
[18] 张涛,张跟喜,张向前,等.京海黄鸡肌细胞生成素基因(MyoG)组织表达谱及表达规律[J].农业生物技术学报,2015,23(5):617-622. ZHANG T,ZHANG G X,ZHANG X Q,et a1.Tissue expression profile and expression pattern of myogenin gene (MyoG) in Jinghai Yellow chicken (Gallus gallus)[J].Journal of Agricultural Biotechnology,2015,23(5):617-622.(in Chinese)
[19] 唐莹,张跟喜,樊庆灿,等.MyoG与Myf5基因与京海黄鸡生长性状的相关分析[J].中国兽医学报,2015,35(9):1562-1567. TANG Y,ZHANG G X,FAN Q C,et al.Association of growth traits with the MyoG and Myf5 genes in the Jinghai Yellow chicken[J].Chinese Journal of Veterinary Science,2015,35(9):1562-1567.(in Chinese)
[20] 赵忠海,李辉,易恒洁,等.MyoG和MEF2a基因多态性聚合效应对鸭屠宰性状的影响[J].中国农业科学,2016,49(18):3649-3661. ZHAO Z H,LI H,YI H J,et al.The effect of MyoG and MEF2a gene pyramiding on slaughter traits of ducks[J].Scientia Agricultura Sinica,2016,49(18):3649-3661.(in Chinese)
[21] 金澄艳.湖羊不同发育阶段IGF-Ⅰ和MyoG基因的表达及与屠宰性状的关联分析[A].中国畜牧兽医学会养羊学分会.2018年全国养羊生产与学术研讨会论文集[C].2018. JIN C Y.Expression of IGF-Ⅰ and MyoG genes in different developmental stages of sheep and their association with slaughtering traits[A].Sheep breeding Branch of China Animal Husbandry and Veterinary Association.Proceedings of the National Symposium on Sheep Production and Academic Production in 2018[C].2018.(in Chinese)
[22] 荣华,豆腾飞,张丽春,等.大围山微型鸡IGF-Ⅰ基因表达量与生长性状的相关性分析[J].中国家禽,2015,37(4):5-8. RONG H,DOU T F,ZHANG L C,et al.Correlation analysis of IGF-Ⅰ gene expression and growth traits in Daweishan Mini chicken[J].China Poultry,2015,37(4):5-8.(in Chinese)
[23] 朱智,徐宁迎,吴登俊,等.鸡IGF-Ⅰ基因SNPs及其对屠体性状的遗传效应分析[J].畜牧兽医学报,2007(10):1021-1026. ZHU Z,XU N Y,WU D J,et al.SNPs of IGF-Ⅰ gene and its genetic effects on carcass traits in chicken[J].Chinese Journal of Animal and Veterinary Sciences,2007(10):1021-1026.(in Chinese)
[24] 胡艳,宋迟,宋卫涛,等.鸭发育早期肝脏IGF-1 mRNA的表达特异性及其与体质量的相关性分析[J].南京农业大学学报,2013,36(6):95-99. HU Y,SONG C,SONG W T,et al.Profiles of hepatic IGF-1 mRNA expression and its correclation with body weight during early development in ducks[J].Journal of Nanjing Agricultural University,2013,36(6):95-99.(in Chinese)
[25] DUCLOS M J.Insulin-like growth factor-Ⅰ(IGF-1)mRNA levels and chicken muscle growth[J].Journal of Physiology and Pharmacology,2005,56(3):25-35.
[26] 宋成创.IGF2基因来源的IGF2 AS和miR-483调控牛骨骼肌细胞增殖分化机制研究[D].杨凌:西北农林科技大学,2019. SONG C C.IGF2 gene-derived IGF2 AS and miR-483 regulate proliferation and differentiation of bovine skeletal muscle cells[D].Yangling:Northwest University of A&F Science and Technology,2019.(in Chinese)

Expression of MyoG and IGF-1 Genes and Its Correlation Analysis with Growth and Slaughter Traits of Yanjin Black-bone Chickens

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Jie, CHEN Chuwen, ZHAO Ruipeng, LIU Yuan, LI Zhixiong. Research Progress on Long Non-coding RNA of Muscle Development in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2427-2438.
[2]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[3]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[4]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[5]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.
[6]	QIANG Hao, LIANG Peng, MENG Ke, RONG Xuan, FENG Dengzhen. Polymorphism of IGF1R Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1765-1785.
[7]	LI Jingyun, WANG Xinle, ZHANG Yunfei, BAI Junyan, LI Gan, LEI Ying, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, WANG Xingke, SIMA Leiyang, ZHANG Shiwen. Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1364-1373.
[8]	WANG Congliang, FU Qi, JU Minying, BAI Jingjing, LI Xinshu, SONG Xiaoyue, LI Longping, LAN Xianyong, QU Lei, ZHANG Lei, ZHU Haijing. The Genetic Polymorphisms of KAP9.2 Gene and Its Correlation Analysis with Growth Traits of Inner Mongolia Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4270-4278.
[9]	FENG Mian, SHANG Mingyu, BI Yazhen, HU Wenping, ZHANG Li. Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3838-3845.
[10]	ZHAO Yuqiang, CHEN Xin, LI Qingchun, MA Jilin, DONG Yinhe, WANG Deming, GUO Lingyun, HUANG Tao. Polymorphism of ENO3 Gene and Its Correlation with Growth Traits in Yorkshire Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 188-196.
[11]	ZHANG Jinhua, SHANG Mingyu, HU Wenping, ZHANG Li. Association Analysis Between Polymorphisms of DGAT1,NR6A1 and MAN1A1 Genes and Growth Traits in Ujumqin Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3312-3322.
[12]	LIU Pengcheng, ZHANG Ji, QIU Ganyuan, GONG Yu, LI Xuesong, LI Wei, ZHANG Yiyu, LIU Ruoyu. Polymorphism of Bovine TBC1D Gene and Its Association with Growth Traits in Guanling Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2853-2863.
[13]	SUN Manxi, CHEN Fang, WANG Zhiyong, LIU Chang, HUANG Shihui, LI Sheng, NIU Xi, WANG Jiafu, RAN Xueqin. Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2448-2456.
[14]	LI Yunxia, YAO Xinkui, MENG Jun, ZENG Yaqi, WANG Jianwen, ZHENG Guopeng, ZHANG Yue, YUAN Xinxin, WANG Tongliang, LI Yuntao. Correlation Analysis Between Polymorphism of MyoG Gene Exon 1 With Meat Quality Traits and Muscle Fiber Traits in Yili Horse [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2504-2511.
[15]	TIAN Quan, WANG Sihan, LIU Lei, ZHAO Yi, LIU Haixing, JIN Huazi, LI Zhongshu, ZHANG Junzheng, JI Shuang. Analysis of MYH3 and MYH8 Genes Polymorphisms and Their Correlation with Growth Traits in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2122-2128.