动物DGAT基因的研究进展

doi:10.16431/j.cnki.1671-7236.2019.07.008

Abstract

Abstract:

DGAT gene includes diacylglycerol acyltransferase 1 (DGAT1) and diacylglycerol acyltransferase 2 (DGAT2) genes,among which the former belongs to ACAT gene family and the latter belongs to MGAT gene family,respectively encoding DGAT1 and DGAT2,which are microsomal enzymes that control the synthesis of triglycerides and transmembrane proteins localized in the endoplasmic reticulum.The membrane topological structure has the ability to interact with other proteins and organelles,affects fat metabolism and lipid deposition in tissues,participates in the regulation of energy synthesis and catabolism in animal body,and affects the metabolism of triglycerides in heart and liver.Meanwhile,the polymorphism of DGAT gene affects the content of fat and lactation.Therefore,it is of great significance to understand the structure and biological function of DGAT gene for the healthy growth,development and production of livestock and poultry.In this paper,the basic structure,biological function and related mechanism of DGAT gene were briefly introduced,and some basic applications of DGAT gene in animal husbandry,such as the regulation of production in mammals,fat deposition and milk fat content,were analyzed.

Key words: diacylglycerol acyltransferase (DGAT); biological function; polymorphism

CLC Number:

S813.1

WU Dongwang, SUN Liyuan, YUAN Zaimei, HE Shichun, HU Chenglong, DENG Mingyue, MAO Huaming, YANG Shuli. Research Progress on Animal DGAT Gene[J]. , 2019, 46(7): 1945-1952.

References

[1] WEISS S B,KENNEDY E P,KIYASU J Y.The enzymatic synthesis of triglycerides[J]. Journal of Biological Chemistry,1960,235(9):40-46.
[2] CASES S,SMITH S J,ZHENG Y W,et al.Identification of a gene encoding an acyl CoA:Diacylglycerol acyltransferase,a key enzyme in triacylglycerol synthesis[J]. Proceedings of the National Academy of Sciences of the United States of America,1998,95(22):13018-13023.
[3] LARDIZABAL K D,MAI J T,WAGNER N W,et al.DGAT2 is a new diacylglycerol acyltransferase gene family:Purification,cloning,and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity[J]. The Journal of Biological Chemistry,2001,276(42):38862-38869.
[4] SMITH S J,CASES S,JENSEN D R,et al.Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking DGAT[J]. Nature Genetics,2000,25(1):87-90.
[5] BUHMAN K K,CHEN H C,FARESE R V.The enzymes of neutral lipid synthesis[J]. Journal of Biological Chemistry,2001,276(44):40369-40372.
[6] CHENG D,MEEGALLA R L,HE B,et al.Human acyl-CoA:Diacylglycerol acyltransferase is a tetrameric protein[J]. Journal of Biological Chemistry,2001,359(3):707-714.
[7] LIU Q,SILOTO R M P,LEHNER R,et al.Acyl-CoA:Diacylglycerol acyltransferase:Molecular biology,biochemistry and biotechnology[J]. Progress in Lipid Research,2012,51(4):350-377.
[8] 马海明,施启顺,柳小春.DGAT相关基因研究进展[J].遗传学报,2005,32(12):1327-1332. MA H M,SHI Q S,LIU X C.Progress in the study on diacylgycerol acyltransferase (DGAT)-related genes[J]. Journal of Genetics and Genomics,2005,32(12):1327-1332.(in Chinese)
[9] MEEGALLA R L,BILLHEIMER J T,CHENG D.Concerted elevation of acyl-coenzyme A:Diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin[J]. Biochemical and Biophysical Research Communications,2002,298(3):317-323.
[10] ANGIOLILLO A,AMILL M,URRUTIA B,et al.Identification of a single nucleotide polymorphism at intron 16 of the caprine Acyl-Coenzyme A:Diacylglycerol acyltransferase 1(DGAT1) gene[J]. Journal of Dairy Research,2007,74(1):47-51.
[11] YEN C L,STONE S J,KOLIWAD S,et al.Thematic review series:Glycerolipids.DGAT enzymes and triacylglycerol biosynthesis[J]. The Journal of Lipid Research,2008,49(11):2283-2301.
[12] CASES S,STONE S J,ZHOU P,et al.Cloning of DGAT2,a second mammalian diacylglycerol acyltransferase,and related family members[J]. The Journal of Biological Chemistry,2001,276(4):38870-38876.
[13] WONGSIRIROJ N,PIANTEDOSI R,PALCZEWSKI K,et al.The molecular basis of retinoid absorption:A genetic dissection[J]. Journal of Biological Chemistry,2008,283(20):13510-13519.
[14] CAO H.Structure-function analysis of diacylglycerol acyltransferase sequences from 70 organisms[J]. BMC Research Notes,2011,249(4):1754-1780.
[15] MCFIE P J,STONE S L,BANMAN S L,et al.Topological orientation of Acyl-CoA:Diacylglycerol acyltransferase-1(DGAT1) and identification of a putative active site histidine and the role of the N terminus in dimer/tetramer formation[J]. Journal of Biological Chemistry,2010,285(48):37377-37387.
[16] BHUMIKA B W,WILLIAM J T,MILLER J H,et al.Role of DGAT enzymes in triacylglycerol metabolism[J]. Archives of Biochemistry and Biophysics,2018,655(3):1-11.
[17] WURIE H R,BUCKETT L,ZAMMIT V A.Evidence that diacylglycerol acyltransferase (DGAT1) has dual membrane topology in the endoplasmic reticulum of HepG2 cells[J]. The Journal of Biological Chemistry,2011,286(42):36238-36247.
[18] BARTON L,BUREŠ D,KOTT T,et al.Associations of polymorphisms in bovine DGAT,FABP4,FASN,and PPARGC1A genes with intramuscular fat content and the fatty acid composition of muscle and subcutaneous fat in Fleckvieh bulls[J]. Meat Science,2016,114:18-23.
[19] CHITRAJU C,MEJHERT N,HAAS J T,et al.Triglyceride synthesis by DGAT1 protects adipocytes from lipid-induced ER stress during lipolysis[J]. Cell Metabolism,2017,26(2):407-418.
[20] NGUYEN T B,LOUIE S M,DANIELE J R,et al.DGAT1-dependent lipid droplet biogenesis protects mitochondrial function during starvation-induced autophagy[J]. Developmental Cell,2017,42(1):9-21.
[21] HUNG Y H,CARREIRO A L,BUHMAN K K.Dgat1 and Dgat2 regulate enterocyte triacylglycerol distribution and alter proteins associated with cytoplasmic lipid droplets in response to dietary fat[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids,2017,1862(6):600-614.
[22] CHEN H C.Inhibition of triglyceride synthesis as a treatment strategy for obesity:Lessons from DGAT1-deficient mice[J]. Arteriosclerosis Thrombosis and Vascular Biology,2005,25(3):482-486.
[23] 王苗,罗军,许会芬,等.山羊 INSIG 1基因超表达对乳腺上皮细胞中脂质合成的影响[J].畜牧兽医学报,2016,47(9):1806-1816. WANG M,LUO J,XU H F,et al.Effect of INSIG1 overexpression on the lipid synthesis in goat mammary gland epithelial cells[J]. Acta Veterinaria et Zootechnica Sinica,2016,47(9):1806-1816.(in Chinese)
[24] LOPES J L,NOBRE T M,CILLI E M,et al.Deconstructing the DGAT1 enzyme:Binding sites and substrate interactions[J]. Biochimica et Biophysica Acta-Biomembranes,2014,1838(12):3145-3152.
[25] YAMAZAKI T,SASAKI E,KAKINUMA C,et al.Increased very low density lipoprotein secretion and gonadal fat mass in mice overexpressing liver DGAT1[J]. Journal of Biological Chemistry,2005,280(22):21506-21514.
[26] ROE N D,HANDZLIK M K,LI T,et al.The role of diacylglycerol acyltransferase (DGAT) 1 and 2 in cardiac metabolism and function[J]. Scientific Reports,2018,8(1):4983-4993.
[27] KOLWICZ S C,LIU L,GOLDBERG I J,et al.Enhancing cardiac triacylglycerol metabolism improves recovery from ischemic stress[J]. Diabetes,2015,64(8):2817-2827.
[28] HAEMMERLE G,MOUSTAFA T,WOELKART G,et al.ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR and PGC-1[J]. Nature Medicine,2011,17(9):1076-1085.
[29] CHOI C S,SAVAGE D B,KULKARNI A,et al.Suppression of diacylglycerol acyltransferase-2(DGAT2),but not DGAT1,with antisense oligonucleotides reverses diet-induced hepatic steatosis and insulin resistance[J]. Journal of Biological Chemistry,2007,282(31):22678-22688.
[30] 吴光松,李平,顾丽菊,等.RNA干扰 DGAT 1和 DGAT 2基因在猪肝细胞中的表达[J].河南农业科学,2018,47(8):123-127. WU G S,LI P,GU L J,et al.RNA interference expression of DGAT 1 and DGAT 2 genes in porcine hepatocytes[J]. Journal of Henan Agricultural Sciences,2008,47(8):123-127.(in Chinese)
[31] MILLAR J S.Short-term overexpression of DGAT1 or DGAT2 increases hepatic triglyceride but not VLDL triglyceride or apoB production[J]. The Journal of Lipid Research,2006,47(10):2297-2305.
[32] 兰英.甘油二酯酰基转移酶2(DGAT2)与鹅肥肝TAG合成和分泌的关系研究[D].雅安:四川农业大学,2006. LAN Y.The relationship between diacylgycerol acyltransferase 2 and TAG synthesis and secretion in fatty liver of goose[D].Ya'an:Sichuan Agricultural University,2006.(in Chinese)
[33] 付金秀,郭红威,李超,等.鸡 DGAT 2基因第7外显子多态性与生产性能的关联[J].农业生物技术学报,2016,24(5):689-696. FU J X,GUO H W,LI C,et al.Polymorphism of exon 7 of DGAT 2 gene and its association with growth traits in chicken (Gallus gallus)[J]. Journal of Agricultural Biotechnology,2016,24(5):689-696.(in Chinese)
[34] 杨春华.猪 DGAT 基因的克隆、SNP检测及与生产性状的关联分析[D].武汉:华中农业大学,2008. YANG C H.Cloning,SNP detection and association analysis with production traits of porcine diacylglycerol acyltransferase genes[D].Wuhan:Huazhong Agricultural University,2008.(in Chinese)
[35] 王国富,高树新,张立凡,等.金华猪 DGAT 1、DGAT 2基因发育性表达特点及其与肌内脂肪的关联分析[J].华北农学报,2011,26(5):25-28. WANG G F,GAO S X,ZHANG L F,et al.Developmental expression characteristics of DGAT 1 and DGAT 2 gene and its association analysis with intramuscular fat in Jinhua pig[J]. Acta Agriculturae Boreali-Sinica,2011,26(5):25-28.(in Chinese)
[36] 王国富,吴慧光,孙国权,等.中国西门塔尔牛 DGAT 1和 DGAT 2基因组织表达谱及其在脂肪组织中的表达与背膘厚的关联分析[J].华北农学报,2012,27(5):60-64. WANG G F,WU H G,SUN G Q,et al.The tissues profile of DGAT1 and DGAT2 and the association analysis of their expression in the adipose with backfat thickness in Chinese Simmental cattle[J]. Acta Agriculturae Boreali-Sinica,2012,27(5):60-64.(in Chinese)
[37] 房兴堂,徐海霞,陈宏,等.徐淮白山羊 DGAT 2基因内含子3多态性与生长性状关联分析[J].生物技术通讯,2009,20(6):814-817. FANG X T,XU H X,CHEN H,et al.Association analysis on polymorphisms of DGAT 2 gene intron 3 with growth traits in Xuhuai White goat[J]. Letters in Biotechnology,2009,20(6):814-817.(in Chinese)
[38] 余刚,罗军,刘俊霞,等.陕北白绒山羊 DGAT 1基因外显子14多态性及其与部分生长和胴体性状的关系[J].西北农林科技大学学报(自然科学版),2008,36(5):1-6. YU G,LUO J,LIU J X,et al.Analysis on the polymorphisms in the exon 14 of DGAT 1 gene and its relationship with some growth and carcass traits in Shaanbei White-cashmere goat[J]. Journal of Northwest A & F University (Natural Science Edition),2008,36(5):1-6.(in Chinese)
[39] HILL R,CANAL A,BONDIOLI K,et al.Molecular markers located on the DGAT1,CAST,and LEPR genes and their associations with milk production and fertility traits in Holstein cattle[J]. Genetics & Molecular Research Gmr,2016,15(1):7794.
[40] DE FREITAS A C,DE CAMARGO G M,STAFUZZA N B,et al.Genetic association between SNPs in the DGAT 1 gene and milk production traits in Murrah buffaloes[J]. Tropical Animal Health and Production,2016,48(7):1421-1426.
[41] 鄢胜飞,尚江华,黄丽华,等.摩拉水牛二酰甘油酰基转移酶2基因的多态性检测[J].中国畜牧兽医,2018,45(10):2787-2796. YAN S F,SHANG J H,HUANG L H,et al.SNP detection of DGAT 2 gene in Murrah buffalo[J]. China Animal Husbandry & Veterinary Medicine,2018,45(10):2787-2796.(in Chinese)
[42] LU J,BOEREN S,HOOIJDONK T V,et al.Effect of the DGAT1,K232A genotype of dairy cows on the milk metabolome and proteome[J]. Journal of Dairy Science,2015,98(5):3460-3469.
[43] MARTIN P,PALHIERE I,MAROTEAU C,et al.A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content[J]. Scientific Reports,2017,7(1):1872-1886.
[44] 谢建鹏,石斌刚,胡江,等.牦牛 DGAT 2基因intron5和intron6多态性及其对乳品质性状的影响[J].农业生物技术学报,2017,25(5):758-769. XIE J P,SHI B G,HU J,et al.Polymorphism in the intron5 and intron6 of DGAT 2 gene and its association with milk quality traits in yak (Bos grunniens)[J]. Journal of Agricultural Biotechnology,2017,25(5):758-769.(in Chinese)
[45] 刘贤慧,常玲玲,吴正常,等.荷斯坦牛二酰基甘油酰基转移酶1基因外显子8的单核苷酸多态性位点对蛋白质结构和功能的影响及其进化分析[J].中国畜牧兽医,2013,40(7):14-17. LIU X H,CHANG L L,WU Z C,et al.Effect of Holstein cows diacylglycerol acyltransferase1 gene in exon 8 on the protein structure and function of single nucleotide polymorphism and its relationship with its evolution analysis[J]. China Animal Husbandry & Veterinary Medicine,2013,40(7):14-17.(in Chinese)
[46] 高小莉,胡江,郭淑珍,等.牦牛 DGAT 1基因多态性及其与乳质性状关联分析[J].中国农业科学,2017,50(16):3215-3225. GAO X L,HU J,GUO S Z,et al.Polymorphisms of DGAT 1 gene and their association with milk quality traits in yak[J]. Scientia Agricultura Sinica,2017,50(16):3215-3225.(in Chinese)
[47] 王凯,柳广斌,黄特民,等.中国南方荷斯坦奶牛 TRAPPC9、DGAT1、GHR、ATP1A 1基因多态性与产奶性状相关性分析[J].华南农业大学学报,2016,37(4):7-12. WANG K,LIU G B,HUANG T M,et al.Associations of polymorphisms in TRAPPC9,DGAT1,ATP1A 1 and GHR genes with milk production traits in Holstein dairy cow in Southern China[J]. Journal of South China Agricultural University,2016,37(4):7-12.(in Chinese)
[48] 巴塔拉伊.奶牛和水牛的 MAP4K 4和 DGAT 1基因对产奶性能和体细胞评分的影响与机理的研究[D].武汉:华中农业大学,2017. DINESH BHATTARAI.Founction and mechanism of MAP4K 4 and DGAT 1 genes on milk traits and SCS between dairy cows and buffalo[D].Wuhan:Huazhong Agricultural University,2017.(in Chinese)

Research Progress on Animal DGAT Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[4]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[5]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[6]	CHEN Jun, CAI Haiming, LIAO Shenquan, QI Nanshan, LI Juan, LYU Minna, LIN Xuhui, HU Junjing, ZHANG Jianfei, LIU Wenjun, SUN Mingfei. Review on the Research of DNA Helicase Family in Parasitic Protozoa [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 317-327.
[7]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[8]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[9]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[10]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[11]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[12]	LI Qilong, CHEN Xiangnan, LONG Feng, CHEN Jiayue, YANG Sen, WANG Jing, ZAN Linsen, CHENG Gong. Research Progress in Function of Snail Family Genes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1287-1294.
[13]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[14]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[15]	WEI Wenzhuo, LIANG Zhenhua, WU Yan, LIU Jingbo, PI Jinsong, ZHANG Hao. Effects of Exosomes on Animal Intestinal Health and Its Research Methods [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 579-586.