仔猪腹泻致病性大肠杆菌分型鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.10.025

Abstract

Abstract:

To understand the epidemic situation and drug resistance of pathogenic Escherichia coli (E.coli) in diarrhea piglets from large-scale swine farms in Guizhou province,agglutination test,PCR and drug sensitive agar diffusion methods were used to perform serotyping and virulence gene classification and drug resistance analysis of 78 pathogenic E.coli isolates in this experiment.The results showed that the main serotypes of 78 pathogenic E.coli were O138 and O87,accounting for 60.8% of the established strains;The virulence genes were detected in 62 strains of pathogenic E.coli,the detection rate was 79.5%,and could be divided into 8 virulence gene types,belonging to enteropathogenic E.coli (EPEC),enterotoxigenic E.coli (ETEC) and enterohemorrhagic E.coli (EAEC),respectively.The virulence genes eaeA,elt and escV had higher detection rate,which were 38.5%,28.2% and 21.8%,respectively.The isolated pathogenic E.coli was highly resistant to β-lactam drugs,and was multi-drug resistant,with more than 8 drug-resistant species.The results showed that the pathogenicity of E.coli in piglets with diarrhea in large-scale pig farms in Guizhou province was high,and the genotypes were complex.The pathogenicity of E.coli was severe.This experiment could provide basic data and theoretical basis for prevention and control of piglets diarrhea in large-scale pig farms.

Key words: piglet diarrhea; pathogenic E.coli; serotype; virulence genes; drug resistance

CLC Number:

S852.61

LIU Lijuan, MA Guangqiang, ZHANG Shengzhan, WANG Wei, WANG Kaigong, ZHOU Bijun, GOU Wanli. Classification and Drug Resistance Analysis of Pathogenic Escherichia coli in Diarrhea Piglets[J]. , 2018, 45(10): 2876-2884.

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Classification and Drug Resistance Analysis of Pathogenic Escherichia coli in Diarrhea Piglets

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