硒化大蒜多糖对环磷酰胺所致免疫抑制鸡的颉颃作用

doi:10.16431/j.cnki.1671-7236.2018.10.026

Abstract

Abstract:

In order to observe the antagonistic effect of selenizing garlic polysaccharide on immunosuppressed chicken induced by cyclophosphamide,336 11-day-old roman chickens were randomly divided into seven groups:Blank control group (BC),vaccine group (VC),cyclophosphamide group(Cy),sGPS3 group,sGPS5 group,sGPS6 group and drug control group (APS),with 6 duplications per group and 8 chickens per duplication.Each chicken except that in BC and VC groups were intramuscularly injected with 8 mg/mL cyclophosphamide 0.5 mL,while each chicken in BC and VC groups were injected with the same amount of saline,once a day for three successive days.At the 14 d of age,The chickens except BC group were vaccinated with ND vaccine,repeated vaccination at 28 days old.At the first vaccination,the chickens in three sGPSs groups were injected with 0.5 mL of 1 mg/mL sGPS₃,sGPS₅ and sGPS₆,respectively.The chickens in APS group were intramuscularly injected with 0.5 mL Astragalus polysaccharide,while that in Cy,VC and BC groups were injected with the same amount of saline,once a day for three successive days.Six chickens were selected randomly from each group for collecting wing venous blood on 7,14,21 and 28 days after the first vaccination,and the serum ND-HI antibody titer were determined by β-micro method,IFN-γ and IL-2 contents were determined by ELISA.The body weighed,thymus,spleen,bursa of Fabricius were collected after culling,and the immune organ indexes were calculated.The result showed that the serum ND-HI antibody titer,IFN-γ and IL-2 contents and bursa of Fabricius index of each administration groups were higher than that of the Cy group (P < 0.05);the serum ND-HI antibody titer and IFN-γ contents at 21 d,IL-2 content at 21 and 28 d in sGPS₆ group were significantly higher than that of other groups (P < 0.05);At 21 and 28 d after first vaccination,the body weight and bursa of Fabricius index in sGPS₆ group were significantly higher than that of other three administration groups (P < 0.05).At 28 d after first vaccination,the thymus and spleen indexes in sGPS₆ group were significantly higher than that of other three administration groups (P < 0.05).In conlusion,the selenizing garlic polysaccharide had the antagonistic effect on immunosuppressed chicken induced by cyclophosphamide,and sGPS₆ had the better effect which could be used as candidate drug for novel immunosuppressive antagonists.

Key words: selenizing garlic polysaccharide; immunosuppression; antibody titer; IFN-γ; IL-2; organ index

CLC Number:

S853.74

QIU Shulei, YANG Haifeng, CHEN Xiaolan, LIU Yunzhen, GUO Changming, YAO Jing, MENG Ting, ZHANG Yixin, WU Caihong. The Antagonistic Effect of Selenizing Garlic Polysaccharide on Immunosuppressed Chicken Induced by Cyclophosphamide[J]. , 2018, 45(10): 2885-2893.

References

[1] 史若男,李婉,宋丽婷,等.动物免疫抑制研究进展[J].动物医学进展,2014,35(9):93-96.SHI R N,LI W,SONG L T,et al.Progress on animal immunosuppression[J].Progress in Veterinary Medicine,2014,35(9):93-96.(in Chinese)
[2] 高玉龙,秦立廷,王笑梅,等.禽病毒性免疫抑制病流行特点与防控对策[J].中国家禽,2012,34(15):5-11.GAO Y L,QIN L T,WANG X M,et al.The epidemic characteristics and prevention,control countermeasures of poultry viral immunosuppression diseases[J].China Poultry,2012,34(15):5-11.(in Chinese)
[3] 武艳,孙莉.毛皮动物免疫抑制的危害及防控措施[J].中国畜牧兽医文摘,2013,29(3):119.WU Y,SUN L.The harm and control measures of immunosuppression in fur animals[J].Chinese Animal Husbandry and Veterinary Abstracts,2013,29(3):119.(in Chinese)
[4] 周泽伟,刘浩,王凯,等.黄芪灵芝口服液对雏鸡免疫功能的影响[J].中国兽医杂志,2016,8(52):59-61.ZHOU Z W,LIU H,WANG K,et al.The effect of Astragalus ganoderma oral liquid on immune function of chicken[J].Chinese Journal of Veterinary Medicine,2016,8(52):59-61.(in Chinese)
[5] 蒋蕊,考书娟,徐小娟,等.槲皮素对免疫低下小鼠体液免疫功能的影响[J].青岛农业大学学报(自然科学版),2011,28(2):118-120.JIANG R,KAO S J,XU X J,et al.Effect of quercetin on humoral immunity function in immunosuppressive mice[J].Journal of Qingdao Agricultural University (Natural Science),2011,28(2):118-120.(in Chinese)
[6] 赵雅.全真一气汤复方多糖对免疫抑制小鼠免疫功能影响初探[D].广州:广州医科大学,2014.ZHAO Y.Preliminary research on Quanzhen Yiqi decoction polysaccharide for the Immune function of immunosuppressed mice[D].Guangzhou:Guangzhou Medical University,2014.(in Chinese)
[7] 贾宁,王汉,郑晶.复方党参提取物对环磷酰胺处理小鼠免疫功能的调节作用[J].中国实验方剂学,2011,9(17):206-209.JIA N,WANG H,ZHENG J.Immunomodulative effect of compound codonopsis radix extract on immunosuppressive mice induced by cyclophosphamide[J].Chinese Journal of Experimental Traditional Medical Formulae,2011,9(17):206-209.(in Chinese)
[8] 郑敏,潘世斌,姜有定,等.大蒜多糖对肝损伤小鼠血清和肝组织ALT、AST的影响程度及急性毒性试验[J].咸宁学院学报,2003,17(2):85-87.ZHENG M,PAN S B,JIANG Y D,et al.An acute toxicity testing of Allium sativum L.polysaccharide and its effects on the ALT and AST in injured mice serum or liver[J].Journal of Xianning College,2003,17(2):85-87.(in Chinese)
[9] MA X,GUO Z,WANG D,et al.Effects of sulfated polysaccha-rides and their prescriptions on immune response of ND vaccine in chickens[J].Carbohydrate Polymers,2010,82:9-13.
[10] 胡群宝,郭宝江.螺旋藻硒多糖对小鼠免疫功能的影响[J].中国海洋药物,2001,5:18-20.HU Q B,GUO B J.Analysis of spirulina-selenium polysaccharide improvement on mice immunological competence[J].Journal of Chinese Marine Drugs,2001,5:18-20.(in Chinese)
[11] STAAF M,YANG Z N,HUTTUNEN E,et al.Structural elucidation of the viscous exopolysaccharide produced by Lactobacillus helveticus Lb161s[J].Carbohydrate Research,2000,326(2):113-119.
[12] 李晓坤,闫吉昌,崔晓莹,等.硒多糖的研究与应用进展[J].中草药,2006,37(7):7-8.LI X K,YAN J C,CUI X Y,et al.Research progress and application of selenium polysaccharides[J].Chinese Traditional and Herbal Medicine,2006,37(7):7-8.(in Chinese)
[13] 杨晶晶,黄家锟,蒋开年,等.正交设计法优化红景天多糖硒制备工艺及其性质研究[J].中成药,2010,32(6):1065-1067.YANG J J,HUANG J K,JIANG K N,et al.The research of optimization process for selenizing Rhodiola polysaccharides according to orthogonal test and its character[J].Chinese Traditional Patent Medicine,2010,32(6):1065-1067.(in Chinese)
[14] 邱树磊.硒化大蒜多糖和硒化枸杞多糖的增强免疫和抗氧化活性研究[D].南京:南京农业大学,2014.QIU S L.The immune-enhancing and antioxidative activities of selenizing garlic polysaccharide and selenizing lyxium polysaccharide[D].Nanjing:Nanjing Agricultural University,2014.(in Chinese)
[15] 郭利伟.中药多糖复方免疫増强剂AEI及其作用机理研究[D].南京:南京农业大学,2012.GUO L W.Studies on compound Chinese herbal medicinal polysaccharide immunopotentiator AEI and its action mechanism[D].Nanjing:Nanjing Agricultural University,2012.(in Chinese)
[16] WANG H,ZHANG D,ZHANG L,et al.The research of preparation and immunology of selenizing Ganoderma lucidum polysaccharides[J].Journal of Food Science,2009,30(17):316-318.
[17] QIN T,CHEN J,WANG D,et al.Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity[J].Carbohydrate Polymers,2013,92(1):645-650.
[18] GUO L,WANG D Y,HU Y L,et al.Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine[J].International Journal of Biological Macromolecules,2012,50(3):512-517.
[19] FAN Y,HU Y,WANG D,et al.Epimedium polysaccharide and propolis flavone can synergistically stimulate lymphocyte proliferation in vitro and enhance the immune responses to ND vaccine in chickens[J].International Journal of Biological Macromolecules,2010,47(2):87-92.
[20] 周建强,潘琦,王涛,等.党参多糖对免疫雏鸡抗体效价和淋巴细胞增殖的影响[J].江苏农业科学,2010,3:263-264.ZHOU J Q,PAN Q,WANG T,et al.Effects of Codonopsis pilosula polysaccharides on antibody titer and lymphocyte proliferation in chickens[J].Jiangsu Agricultural Sciences,2010,3:263-264.(in Chinese)
[21] 周泽伟,李楠,余峥嵘,等.黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响[J].中国畜牧兽医,2015,42(7):1859-1864.ZHOU Z W,LI N,YU Z R,et al.Effect of the oral liquid of Astragalus and Lucidum polysaccharides on immunological function in chichens immuned with ND vaccine[J].China Animal Husbandry & Veterinary Medicine,2015,42(7):1859-1864.(in Chinese)
[22] FAN Y,LU Y,WANG D,et al.Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chickens[J].Cellular Immunology,2013,281(1):37-43.
[23] THAKUR A,PEDERSEN L E,JUNGERSEN G.Immune markers and correlates of protection for vaccine induced immune responses[J].Vaccine,2012,30(33):4907-4920.
[24] 于晓东,王立辛.连翘苷拮抗环磷酰胺所致小鼠免疫抑制的实验研究[J].中国免疫学杂志,2017,33(8):1177-1180.YU X D,WANG L X.Experimental study on phillyrin resist mice immunosuppression induced by cyclophosphamide[J].Chinese Journal of Immunology,2017,33(8):1177-1180.(in Chinese)
[25] 杨汉春.动物免疫学[M].北京:中国农业出版社,2002.YANG H C.Animal Immunology[M].Beijing:China Agriculture Press,2002.(in Chinese)
[26] 陈兴颖.中药多糖免疫增强剂芪藿饮及作用机理研究[D].南京:南京农业大学,2014.CHEN X Y.Studies on Chinese herbal medicinal polysaccharide immunopotentiator AEO and its mechanism[D].Nanjing:Nanjing Agricultural University,2014.(in Chinese)
[27] 宋玉龙,丘富安,吴秀钦,等.硒化修饰太子参多糖对免疫损伤小鼠的免疫保护作用[J].中国兽医学报,2017,11(37):2163-2180.SONG Y L,QIU F A,WU X Q,et al.Immunoprotection effect of selenium radix Pseudostellaria polysaccharide on immune injured mice[J].Chinese Journal of Veterinary Science,2017,11(37):2163-2180.(in Chinese)
[28] 林丹丹,秦韬,任喆,等.硒化党参多糖对免疫抑制小鼠免疫功能的影响[J].中国畜牧兽医,2016,43(6):1544-1549.LIN D D,QIN T,REN Z,et al.Effects of selenium Codonopsis pilosula polysaccharide on immune function of immunosuppressed mice[J].China Animal Husbandry & Veterinary Medicine,2016,43(6):1544-1549.(in Chinese)

The Antagonistic Effect of Selenizing Garlic Polysaccharide on Immunosuppressed Chicken Induced by Cyclophosphamide

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Qianqian, YANG Wanqun, WANG Yubo, WU Fei, DAI Juanjuan, QIN Mimi, ZHUANG Jinqiu, WANG Yan, SHEN Zhiqiang, LI Jichang. Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 693-703.
[2]	SHI Jiancun, LI Jikai, JIA Lin, LYU Jiancun, SHANG Zihan, ZHANG Tie, WANG Chunguang. Effects of Fermented Traditional Chinese Medicine on the Growth Performance, Antibody Level and Intestinal Flora of Laying Hens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2145-2155.
[3]	ZHANG Jinghan, LI Zhilan, HAN Diangang, HE Shuai, LIU Jinhua, LYU Nianci, ZOU Fengcai, CHAI Jun, ZHANG Yifang. Construction of PK-15 Cells Stably Expressing IL-10 and Its Application in PCV2 Proliferation [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1085-1095.
[4]	LI Xunsheng, SHI Wanwan, XING Baorui, ZHOU Jue, LI Chunyi, SUN Hongmei. Research Progress on the Biological Function of Galectin-1 and Its Role in Deer Antler Regeneration [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3746-3755.
[5]	GUO Linxia, MA Kewei, FENG Zhihua, LIU Yanci, LIU Guanzhong, LI Qingyan, GONG Sumei, LI Shupeng, ZHAO Guoxian. Effects of Jujube Polysaccharide on Growth Performance and Immune Indexes of Immunosuppressive Egg-type Chicks [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3264-3272.
[6]	GONG Lei, LANG Xi, MA Sha, CHEN Cui, ZHOU Zhenghong. Effects of Single High Dose and Multiple Low Dose Cyclophosphamide Abdominal Injection on Immunosuppressive Model in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3787-3794.
[7]	YAN Yanhua, CHEN Tong, CHEN Zewen, XUE Yang, DU Fangfang, ZHANG Hongying, TONG Chao, WANG Xuebing. Study on the Preventive and Therapeutic Effect of Compound Banqing Soluble Powder on Chicken Infectious Bronchitis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3379-3388.
[8]	LIU Sheng, ZHANG Jianan, JIA Ning, FANG Mei, DENG Kang, HUANG Weikuan. Analysis of Active Components of Total Flavonoids from the Seeds of Ammopiptanthus mongolicus and Its Effects on Immune Function in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2726-2736.
[9]	LIU Meifen, LI Qinnan, MA Zhenyuan, WU Zhiming, BAN Fuguo, YAN Ruoqian, WANG Huajun. Immunopotentiation Effect of Recombinant Chicken Interleukin-6/2 Fusion Protein on Newcastle Disease Virus (LaSota) Live Vaccine [J]. , 2019, 46(2): 590-599.
[10]	YAO Lili, BAI Lin, TAN Ying, SUN Jiaqi, SHI Dayou, GUO Shining, LIU Cui. Study on Antagonistic Effects of Echinacea purpurea Polysaccharide and Sulfated Echinacea purpurea Polysaccharide on Immunosuppression of Cyclophosphamide in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3084-3094.
[11]	JI Hailong, WANG Hongying, ZHOU Yingnan, QIAN Siriguleng. Effects of Rumen-derived Probiotics on the Growth and Physiological Characteristics of Mice [J]. , 2018, 45(9): 2463-2470.
[12]	ZHANG Xiaojing, SHI Hongtao, NIU Xiangnan, SONG Yuzhen, TANG Fayin, BIAN Chuanzhou, QIAO Hongxing. Effect of Aqueous Extract of Fermented Astragalia and Glycyrrhiza on Growth Performance, Immune Organ Indexes and Meat Quality of 817 Broilers [J]. , 2018, 45(4): 933-939.
[13]	ZHU Ling, LIU Xuwei, YAN Wenliang, ZHANG Rui, HAN Xiaoli, YAN Genqiang. Screening and Identification of Bovine Capsular Type A Pasteurella multocida Vaccine Candidate Strains [J]. , 2018, 45(2): 471-478.
[14]	WANG Shunan, DONG Li, WEI Zhenwu, HUO Yongjiu, FANG Wei, FENG Feifei, ZHU Xinyu, YU Lihuai. Effects of Tea Tree Oil on Growth Performance,Serum Biochemical and Viscera Indexes of Weaned Piglets [J]. , 2018, 45(1): 86-92.
[15]	LIU Yan, PENG Wen-jing, XIN Rui-hua, ZHENG Ji-fang, LIANG Ge, LUO Yong-jiang. Effect of Herbal Compound Outikang on Antibody Titer of Foot and Mouth Disease Vaccine，Cytokines and Biochemical Indices in Cows [J]. , 2017, 44(4): 1219-1225.