新疆北疆地区猪源粪肠球菌的耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.05.031

Abstract

Abstract:

In order to determine the drug resistance of swine Enterococcus faecalis and the distribution of genotypes resistance in Northern Xinjiang,total 49 isolates were evaluated for their sensitivity to 8 antimicrobial drugs by Kirby-Baller disc diffusion method,and 9 relative resistance genes were detected by PCR method.The amplified fragments were sequenced and compared with the corresponding gene sequences in GenBank.Susceptibility test results showed that the isolates had the highest resistance rate to streptomycin,followed by penicillin and erythromycin,which were highly sensitive to nitrofurantoin and ampicillin.PCR detection results showed that the detection rate of β-lactam resistance gene tem was the highest (93.88%),followed by the tetracycline resistance gene tetM (85.71%),the detection rates of quinolones resistance gene gyrA and parC both were 42.86%,the detection rates of aminoglycoside resistance genes aph(3')-Ⅲ,aac(6')/aph2″ and ant(6')-Ⅰ were 36.73%,16.33% and 16.33%,respectively,and no mefA and ermB genes were detected.The results showed that the multidrug resistance of swine Enterococcus faecalis in Northern Xinjiang was very serious,and its resistance phenotype was not consistent with the genotype.

Key words: Enterococcus faecalis; antibacterials; drug resistance gene

CLC Number:

S852.61

WANG Mengmeng, ZHANG Zirong, OU DU·Wu tu na sheng, PU Jingwei, YUAN Ligang, QI Yayin. Drug Resistance Analysis of Swine Enterococcus faecalis in Northern Xinjiang[J]. , 2018, 45(5): 1374-1381.

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Drug Resistance Analysis of Swine Enterococcus faecalis in Northern Xinjiang

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