China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (1): 240-248.doi: 10.16431/j.cnki.1671-7236.2020.01.028

• Basic Veterinary Medicine • Previous Articles     Next Articles

Isolation,Identification and Detection of Drug Resistance and Drug Resistance Gene of Pulmonary Pathogenic Escherichia coli from Calves

GU Xiaoxiao1, WU Qin1, TAO Qiaoxiaoci1, MA Xue1, LI Jie1, ZHOU Xia1, HAN Mengli2, HUANG Xin2, WU Tongzhong2, ZHANG Xingxing2, ZHONG Fagang2   

  1. 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
    2. National Key Laboratory of Sheep Genetic Improvement and Healthy Aquaculture, Xinjiang Academy of Agricultural Reclamation, Shihezi 832000, China
  • Received:2019-08-02 Online:2020-01-20 Published:2020-01-17

Abstract: The purpose of this study was to identify the bacterial pathogens that cause the death of calves due to respiratory diseases in a cattle farm in Shihezi,Xinjiang,and to analyze the phylogenetic classification,drug resistance phenotype and drug resistance gene of the isolated pathogens.The routine isolation of bacteria combined with automatic biochemical analysis system was used to isolate and identify the isolates.The paper and PCR methods were used to analyze the systematic grouping,drug sensitive phenotype and drug resistance genes of the isolates.Six strains of pathogenic E.coli were isolated and identified from the lung,liver and lymph nodes of infected calves.All of the six strains were multidrug resistant.The drug resistance rates of penicillin,amoxicillin,cefazolin,cefradine,streptomycin,gentamicin,florfenicol,teicoplanin,clindamycin,meropenem,tetracycline and tobramycin were 100%.The carrying rates of aminoglycoside resistance genes aacC2,β-lactam resistance genes blaCTX-M and blaTEM,tetracycline resistance gene tetA,quinolone resistance gene gyrA,and sulfonamide resistance gene sul2 were 66.67%,83.3%,66.7%,100%,100% and 100%,respectively.E.coli was the bacterial pathogen causing respiratory tract infections in dead calves in a cattle farm in Shihezi,Xinjiang.It showed strong multi-drug resistance,and the isolates carried more abundant drug resistance genes.

Key words: bovine respiratory infection; pathogenic Escherichia coli; drug resistance; drug resistance gene

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