重庆部分羊场沙门氏菌的分离鉴定、耐药表型分析及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2020.07.032

Abstract

Abstract: The purpose of this research was to acquire the situation of drug resistance and resistance genes prevalence of Salmonella isolated from goats in Chongqing.185 fecal samples were collected from 5 goat farms.Salmonella were isolated by selective enrichment and PCR,and their serotypes were determined.Then,the antimicrobial susceptibility of the isolates to 28 antimicrobial agents was measured.The mutations of quinolone resistance-determining region (QRDR),plasmid-mediated quinolone resistance (PMQR) and extended-spectrum β-lactamases (ESBL) genes were also detected.A total of 11 Salmonella isolates were obtained from goats,and 10 of them were S.Derby.The isolated strains were more resistant to ampicillin,cefazolin,cefalexin,cefotaxime,tetracycline and pefloxacin,and 9 strains showed multi-drug resistant to 3-7 classes of antimicrobial agents.All strains had QRDR resistant mutations and carried bla_TEM gene,whereas,7 strains carried 1-5 PMQR genes.On the whole,Salmonella isolated from goats were resistant to common used antimicrobials.Meanwhile,drug resistant mutations and drug resistant genes were widespread in the isolates.

Key words: goats; Salmonella; antimicrobial susceptibility; serotype; drug resistance gene

CLC Number:

S855.1

FENG Lin, WANG Xudong, LI Jiubin, DING Honglei, YANG Yujiao. Isolation,Identification,Drug-resistant Phenotype Analysis and Drug Resistant Gene Detection of Salmonella at Goat Farms in Chongqing[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2256-2263.

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Isolation,Identification,Drug-resistant Phenotype Analysis and Drug Resistant Gene Detection of Salmonella at Goat Farms in Chongqing

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