›› 2018, Vol. 45 ›› Issue (5): 1382-1388.doi: 10.16431/j.cnki.1671-7236.2018.05.032

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Study on Cordycepin Prevents Obesity and Mediates Prolactin Secretion Through ADORA1 in GH3 Cells

LI Yan1, XU Hongyue1, LI Shulin1, WANG Xueyan2, TANG Xudong2, YU Lu1   

  1. 1. Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China;
    2. Key Laboratory of New Drug Research and Development, Institute of Shenzhen, Tsinghua University, Shenzhen 518057, China
  • Received:2017-12-08 Online:2018-05-20 Published:2018-05-19

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Abstract:

To investigate the effect of cordycepin on preventing obesity and its possible mechanism,high-fat-diet induced rat models were used.Experimental groups were given daily oral administration of cordycepin at different concentrations (50,25 and 12.5 mg/(kg·d)) for 35 days.Subsequently,body weight,fat weight and serum lipids of obese rats induced by high-fat diet were selected as the indexes to observe the effect of different dosages of cordycepin on preventing obesity.The role of cordycepin on cell proliferation,expression of adenosine receptor A1 (ADORA1) and the secretion of prolactin were detected by CCK8,Western blotting and ELISA in GH3 cells.The results in vivo showed that compare with normal control group,the body weight,fat weight and the level of serum lipids(except HDL) in modle rats were extremely significantly incresed (P<0.01),the body weight,fat weight,and the levels of TC,TG and LDL in rats administrated with cordycepin were extremely significantly or significantly reduced (P<0.01;P<0.05),and the level of HDL was significantly increased in high-dose cordycepin group (P<0.05),there was no significant difference between low-dose cordycepin and middle-dose groups (P>0.05).The results in vitro showed that it had no effect on the proliferation when the concentration of cordycepin was lower than 25 μg/mL in GH3 cells;The expression of ADORA1 in GH3 cells was increased by cordycepin induction,the secretion of prolactin could be significantly or extremely significantly reduced by cordycepin and ADORA1 agonist (R-PIA) (P<0.05;P<0.01),but its effect could be blocked by ADORA1 inhibitor (DPCPX).In conclusion,cordycepin could prevent obesity and reduce serum lipids,which might be related to inhibiting the secretion of prolactin by ADORA1.The results would lay a theoretical foundation for the development of anti-obesity drugs.

Key words: cordycepin; obesity; serum lipids; prolactin; GH3 cell

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