牛源致病性大肠杆菌的敏感药物筛选及腐殖酸钠的抑菌试验

doi:10.16431/j.cnki.1671-7236.2020.10.036

Abstract

Abstract: In order to get more information about the distribution of virulence genes and drug resistance genes of Escherichia coli(E.coli) isolated from calf diarrhea in some large-scale cattle farms in Heilongjiang,sensitive drugs were screened to analyze the preventive and therapeutic effect of sodium humate on E.coli.In this study,40 samples were collected and identified by separation and purification,staining microscopy,biochemical identification and molecular biology,and the virulence and drug resistance genes were detected by PCR and the drug sensitivity was detected by K-B test.The inhibitory effect of different concentrations of sodium humate on E.coli was measured by serial dilution and plate counts.Moreover,the protective effect of sodium humate on mice were studied through gastric administration.The results showed that 30 strains of E.coli were isolated,of which 25 strains had virulence genes and 8 virulence genes were detected out of 10,with detection rate of 83.3%.There were many virulence gene combinations.The highest detection rates for bla_TEM and bla_SHV genes were 100% and 13.3% respectively,and the detection rate of other resistance genes was also high.The results of drug sensitive test showed that 30 isolated strains were most sensitive to meropenem,followed by amikacin.The results of serial dilution and plate counts showed that 5% sodium humate was the most bacteriostatically effective,and the results of toxicity test showed that sodium humate could improve the survival rate of mice.This study provided a basis for the rational use of antibiotics and the clinical application of sodium humate in this area.

Key words: Escherichia coli; virulence genes; drug resistance genes; antimicrobial susceptibility test; sodium humate

CLC Number:

S853

WANG Dong, JIN Shengzi, DU Yuanyi, GUAN Tongxu, LI Wanying, LIU Yun. Screening of Sensitive Drugs for Bovine Pathogenic Escherichia coli and Bacteriostatic Test of Sodium Humate[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3361-3370.

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Screening of Sensitive Drugs for Bovine Pathogenic Escherichia coli and Bacteriostatic Test of Sodium Humate

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