中药复方对大肠杆菌感染雏鸭的防治效果研究

doi:10.16431/j.cnki.1671-7236.2020.10.037

Abstract

Abstract: In this study,the clinical therapeutic effect of Chinese herbal compound composed of Euphorbia humifusa Willd.and Punica granatum L.on ducklings infected with Escherichia coli (E.coli) was studied in order to provide a new therapeutic method for clinical treatment of E.coli.Fifty healthy one-day-old ducklings were selected and randomly divided into five groups,ten ducklings in each group.The experimental groups were blank control group,model group,amoxicillin group,Chinese medicine compound high-dose group(DS_H) and low-dose group(DS_L).The administration group started administration at 3 days of age,continued administration for 3 days,and continued administration until the end of the experiment,10 days total.Except for control group,when the ducklings were 6 days old,E. coli was intraperitoneally injected for challenge,0.5 mL/head.The mortality of the experimental groups was compared.The dead ducks and the surviving ducks were dissected,the pathological section of liver and small intestine and HE staining sections were observed.The blood routine indexes and serum biochemical analysis were detected to evaluate the therapeutic effect of traditional Chinese medicine compound on E.coli infected ducklings.The results showed that the high and low doses of the traditional Chinese medicine compound and the amoxicillin could effectively reduce the mortality of infected ducklings.Compared with model group,amoxicillin and traditional Chinese medicine high and low dose groups could reduce liver,small intestine and other organs damage.Compared with control group,aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in model group increased significantly (P<0.05),and alkaline phosphatase (ALP),lactate dehydrogenase (LDH),UREA also showed extremely significant upward trend (P<0.01),and the WBC,RBC,HGB,PLT and other indicators changed extremely significantly(P<0.05).Compared with model group,the alkaline phosphatase(ALP)value was significantly or extremely decreased in the administration group(P<0.05;P<0.01),and the blood WBC,RBC,HGB and PLT indexes were extremely significantly increased (P<0.01).The results indicated that the degree of body damage in the administration group was lower than that in the model group.

Key words: Escherichia coli; ducklings; in vivo; Chinese medicine; treatment effect

CLC Number:

S853.74

QIN Feng, LIU Yun, WU Zhi, FENG Qi, WU Shuang, SONG Haigang, WU Xiaojie, ZHU Shanyuan. Study on the Prevention and Control Effect of Chinese Herbal Compound on E.coli Infected Ducklings[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3371-3378.

References 18

[1]	陆承平.兽医微生物[M].第5版.北京:中国农业出版社,2013. LU C P.Veterinary Microorganisms[M].5th Edition.Beijing:China Agriculture Press,2013.(in Chinese)
[2]	王云.鸡大肠杆菌病的防控措施[J].中国畜牧兽医文摘,2016,32(5):155. WANG Y.Prevention and control measures for chicken colibacillosis[J].Chinese Abstracts of Animal Husbandry and Veterinary Medicine,2016,32(5):155.(in Chinese)
[3]	WANG S,NIU C,SHI Z,et al.Effects of ibeA deletion on virulence and biofilm formation of avian pathogenic Escherichia coli[J].Infection and Immunity,2011,79(1):279-287.
[4]	DHO MOULIN M,FAIRBROTHER J M.Avian pathogenic Escherichia coli (APEC)[J].Veterinary Research,1999,30(2-3):299.
[5]	岳秀英,葛荣,吴晓岚,等.四川省猪、鸡源大肠杆菌对抗生素耐药性研究[J].中国兽医杂志,2017,1:93-95. YUE X Y,GE R,WU X L,et al.Study on antibiotic resistance of Escherichia coli from pigs and chickens in Sichuan province[J].Chinese Journal of Veterinary Medicine,2017,1:93-95.(in Chinese)
[6]	宁官保,牛艺儒,张鼎,等.鸡源大肠杆菌耐药性分析及中药对大肠杆菌耐药性消除作用的研究[J].畜牧兽医学报,2015,46(6):1018-1025. NING G B,NIU Y R,ZHANG D,et al.Studies on the drug resistance of chicken isolated Escherichia coli and role of traditional Chinese medicines in the drug resitance elimination[J].Chinese Journal of Animal and Veterinary Sciences,2015,46(6):1018-1025.(in Chinese)
[7]	HALFAOUI Z,MENOUERI N M,BENDALI L M.Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria[J].Veterinary World,2017,10(7):830-835.
[8]	KOO H J,WOO G J.Distribution and transferability of tetracycline resistance determinants in Escherichia coli,isolated from meat and meat products[J].International Journal of Food Microbiology,2011,145(2):407-413.
[9]	贺常亮,韦旭斌,付本懂,等.抗鸡大肠杆菌O78单味中药及有效成分的筛选[J].中国畜牧兽医,2013,40(6):178-184. HE C L,WEI X B,FU B D,et al,Screening of single Chinese herbal medicines and effective ingredients for anti-chicken E.coli O78[J].China Animal Husbandry & Veterinary Medicine,2013,40(6):178-184.
[10]	张步彩,袁橙,苏治国,等.4味中药复方治疗鸡大肠杆菌病效果的研究[J].中国畜牧兽医,2019,46(9):2803-2812. ZHANG B C,YUAN C,SU Z G,et al.Study on the effect of 4 traditional Chinese medicine compouds on treating colibacillosis of chicken[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2803-2812.
[11]	于苗苗,姜晓文,于文会,等.中药复方对雏鸡大肠杆菌病受损脏器治疗效果[J].黑龙江畜牧兽医,2018,3:215-218. YU M M,JIANG X W,YU W H,et al.Therapeutic effect of traditional Chinese medicine compound on damaged organs of chicken colibacillosis[J].Hei-longjiang Animal Science and Veterinary Medicine,2018,3:215-218.(in Chinese)
[12]	江超,姚飞虹,张之怡,等.地锦草与5种抗菌药联用对猪链球菌的体外抑菌效果[J].安徽农业科学,2019,47(24):114-116. JIANG C,YAO F H,ZHANG Z Y,et al.Antibacterial effect of Euphorbia humifusa combining with five antimicrobials against Streptococcus suis in vitro[J].Journal of Anhui Agricultural Sciences,2019,47(24):114-116.(in Chinese)
[13]	贾艳华,李荣誉,张国祖,等.地锦草与10种抗菌药联用对鸡大肠杆菌的体外抑菌效果[J].黑龙江畜牧兽医,2014,7:126-129. JIA Y H,LI R Y,ZHANG G Z,et al.Antibacterial effect of Euphorbia humifusa combining with five antimicrobials against chicken E.coli in vitro[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2014,7:126-129.(in Chinese)
[14]	王红均,程邓芳,先宏,等.17味中草药及其复方对鸽沙门氏杆菌的体外抑菌试验[J].黑龙江畜牧兽医,2019,15:135-137. WANG H J,CHENG D F,XIAN H,et al.In vitro bacteriostatic test of 17 kinds of Chinese herbal medicine and their compound against Salmonella from pigeon[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2019,15:135-137.(in Chinese)
[15]	柯文杰,覃华斌,陈言峰,等.25种中草药对普通变形杆菌的体外抑菌试验[J].水产科技情报,2019,46(6):328-331. KE W J,QIN H B,CHEN Y F,et al.In vitro antibacterial test of 25 Chinese herbal medicines against common Proteus[J].Fishery Science and Technology Information,2019,46(6):328-331.(in Chinese)
[16]	操刚,赵骥,陈红光,等.当归多糖对小鼠试验性肝损伤的影响[J].湖北科技学院学报(医学版),2004,18(5)5:332-334. CAO G,ZHAO J,CHEN H G,et al.Effect of Angelica sinensis polysaccharide on experimental hepatic injury in mice[J].Journal of Hubei University of Science and Technology(Medical Sciences),2004,18(5):332-334.(in Chinese)
[17]	黄诗琦,韦雅妮,赵雨川,等.鸡血藤总黄酮对大肠杆菌败血症的治疗作用[J].中国畜牧兽医,2019,46(5):297-306. HUANG S Q,WEI Y N,ZHAO Y C,et al.The rapeutic effect of total flavonoid of Spatholobus suberectus Dunn on Escherichia coli sepsis[J].China Animal Husbandry & Veterinary Medicine,2019,46(5):297-306.(in Chinese)
[18]	王华,张鲁滨,张雪冰,等.艾叶水提液增强家兔抗大肠杆菌免疫的研究[J].畜牧兽医学报,2019,50(11):2348-2356. WANG H,ZHANG L B,ZHANG X B,et al.Enhancement of rabbbit immunity against E.coli by oral adminstration of water extract of Artemisia argyi Levl.et vant[J].Acta Veterinary et Zootechnica Sinica,2019,50(11):2348-2356.(in Chinese)

Study on the Prevention and Control Effect of Chinese Herbal Compound on E.coli Infected Ducklings

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