贝莱斯芽孢杆菌发酵对乳康方剂中药有效成分提取效果的影响

doi:10.16431/j.cnki.1671-7236.2023.06.038

Abstract

Abstract: 【Objective】 The experiment was conducted to explore the effect of fermentation by Bacillus velezensis on the content changes of the main active components of the self-prepared Rukang prescription, including the flavonoid glycosides from Vaccaria segetalis, caffeic acid and rutin.【Method】 The study established high performance liquid chromatography(HPLC) methods and investigation method linear range, precision, stability, repeatability, sample recovery for the determination of flavonoid glycosides from Vaccaria segetalis, caffeic acid and rutin in self-prepared Rukang prescription.The fermentation culture medium was prepared with the main ingredients of self-prepared Rukang prescription, such as the powder of Vaccaria segetalis, Taraxacum mongolicum Hand.-Mazz, Astragalus membranaceus, Angelica sinensis, Glycyrrhiza uralensis Fisch., accounting for 30%, soybean powder accounting for 10%, CaCO₃ accounting for 0.2%, and ultra-pure water accounting for 59.8%.The fermentation group was inoculated with 2% (v/w) of Bacillus velezensis seed liquid, mixed evenly, and then fermented.After fermentation, double amount of water was added, repeatedly simmered, and filtered as samples to be tested.The contents of flavonoids glycosides from Vaccaria segetalis, caffeic acid and rutin in the fermented and non-fermented Rukang prescription were determined by HPLC.【Result】 The established HPLC methods for the determination of flavonoid glycosides from Vaccaria segetalis, caffeic acid and rutin in Rukang prescription, the linear relationship was good within the range of 0.01-0.5, 5-120 and 10-100 μg/mL, respectively, and the methodological investigations were good, the established method had good precision, stability, repeatability and recovery rate.Under the same physical conditions, the contents of flavonoids glycosides from Vaccaria segetalis, caffeic acid and rutin in the extract of self-prepared Rukang prescription fermented by Bacillus velezensis were 4.99, 6.86 and 5.81 mg/mL, respectively.The contents of flavonoids glycosides from Vaccaria segetalis, caffeic acid and rutin in the non-fermented self-prepared Rukang prescription were 3.70, 3.01 and 3.27 mg/mL, respectively.The difference between the two groups was extremely significant (P<0.01).The results showed that Bacillus velezensis was suitable for fermenting self-prepared Rukang prescription, and the extraction effect was better than that of non-fermented group.The fermentation of Bacillus velezensis was beneficial to the release of flavonoids glycosides from Vaccaria segetalis, caffeic acid and rutin in self-prepared Rukang prescription.【Conclusion】 The fermentation of self-prepared Rukang prescription by Bacillus velezensis could significantly increase the content of main effective ingredients, which provided a technical reference for the efficient extraction of self-prepared Rukang prescription.

Key words: Bacillus velezensis; lactate; Chinese medicine; ferment; active component

CLC Number:

S859.5

XUE Zhanmei, LIU Xuesong, ZHANG Yan, ZHANG Bei, MENG Weishan, ZHANG Guohua, WANG Yan, ZHONG Peng, QIU Jinghui, TANG Jilong, SHI Tongrui. Effect of Bacillus velezensis Fermentation on Extraction of Active Components from Traditional Chinese Medicine of Rukang Prescription[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2540-2549.

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Effect of Bacillus velezensis Fermentation on Extraction of Active Components from Traditional Chinese Medicine of Rukang Prescription

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