禽源产色葡萄球菌的分离鉴定及其耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.07.027

Abstract

Abstract: In order to understand the resistance of avian-origin Staphylococcus chromogenes,a Staphylococcus chromogenes strain named GZQX2019 was isolated from Jinling chicken.The bacteria were isolated and cultured,Gram staining microscopy,biochemical test,16S rDNA sequence analysis,drug sensitivity test,partial resistance and enterotoxin gene detection and animal regression test.The bacteria separation results showed that the white colony with convex surface and neat edge appeared on the blood agar medium,and the Staphylococcus was detected by Gram staining.The results of biochemical tests showed that the reactions of sucrose,arginine hydrolase and xylose were positive,while urea,xylitol and maltose were negative.The phylogenetic tree showed that the isolation was in the same branch with Staphylococcus chromogenes.The drug sensitivity test showed the isolation was resistant to 6 kinds of antibacterial such as erythromycin,ceftazidime and doxycycline,and sensitive to 11 kinds of antibacterial such as cefuroxime,cefoperazone and neomycin.The results of drug resistance and enterotoxin gene detection showed that Alt,ermB,VIM,TEM and tetB could be detected.The results of animal regression tests showed that the isolates were not pathogenic or non-pathogenic to mice and chicks.The experiment successfully isolated one avian-origin Staphylococcus chromogenes,which enriched the cognition of avian-origin Staphylococcus chromogenes.

Key words: Jinling chicken; Staphylococcus chromogenes; drug resistance gene; virulence gene

CLC Number:

S855.1⁺1

CHEN Guang, WEN Guilan, ZHANG Xiyi, TIAN Lang, YANG Baiqi. Isolation and Identification of Avian-origin Staphylococcus chromogenes and Its Drug Resistance Analysis[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2215-2222.

References

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Isolation and Identification of Avian-origin Staphylococcus chromogenes and Its Drug Resistance Analysis

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