贵州某鸡场大肠杆菌与葡萄球菌混合感染病原的分离鉴定

doi:10.16431/j.cnki.1671-7236.2017.05.030

Abstract

Abstract:

In order to analyze the variety, drug resistance and pathogenicity of bacterial of a chicken farm in Guizhou province, bacterial isolation culture was done from four sick chickens that were submitted by the farm. Gram staining, biochemical test, drug susceptibility test, 16S rDNA sequencing analysis and so on were carried out to identify the bacteria.The results showed that we isolated two strains of bacteria with different colony morphology that one of them was gram-negative bacilli,the other was gram-positive cocci, which were named as GZHX2016-1 and GZHX2016-2 according to the isolated location and time.GZHX2016-1 wasEscherichia coli (E. coli) with multiple drug, and GZHX2016-2 was Staphylococcus simulans which was negative on Staphylococcus coagulase test and was resistant to some antibiotics. The 16S rDNA sequencing of GZHX2016-1 was more close genetic relationship with E. coli (GenBank accession No.:CP007442.1, CP014667, KT156725.1, and so on), and the homology was 99.5%.The GZHX2016-1 was more close genetic relationship with Staphylococci (GenBank accession No.:HM140412.1, AM944030.1, KM877513.1, and so on), and the homology was 97.9%. GZHX2016-1 and GZHX2016-2 were pathogenic to mouse. The minimum lethal dose of the GZHX2016-1 was 1.12×10⁸ CFU of each mouse by intraperitoneal injection, and the minimum lethal dose of GZHX2016-2 was 3.20×10⁷ CFU.In summary, the experiment successfully isolated a strain of pathogenic E. coli and a strain of coagulase-negative Staphylococcus from chickens.The chicken farm was infected by E. coli andStaphylococcus,and the bacteria were resistant to multiple antibiotics. So, we should take effective measures to control the bacteria pollution in the chicken farm, and restrict the use of antibiotics to reduce the resistance bacteria, and ensure the food safety of chicken and chicken products.

Key words: i>Staphylococcus simulans; Escherichia coli; biochemical identification; 16S rDNA

CLC Number:

S858.31

CHEN Shao-pin, WEN Gui-lan, ZHANG Sheng-bo, WU Chang-hua, LIN Han-qing, FAN Chang-long, GUAN Guo-dan, WANG De-sheng, WEN Ming, ZHOU Bi-jun, CHENG Zhen-tao. Isolation and Identification of Pathogen ofEscherichia coli andStaphylococcus Mixed Infection of Chichen in Guizhou Province[J]. , 2017, 44(5): 1468-1476.

References

[1] 恽时锋,潘震寰,郑明球.鸡大肠杆菌病研究进展[J]. 畜牧与兽医,1998, 30(6):277-280.
[2] 索朗扎西,范云鹏,麻武仁,等. 锦珠草颗粒对人工感染鸡大肠杆菌病的防治试验[J]. 中国兽医学报,2015,35(2):319-324.
[3] 林维庆.鸡大肠杆菌病[J].华南农业大学学报,1991,12(3):7-13.
[4] Wu Q, Li Y, Hu H, et al. Rapid identification of Staphylococcus aureus:FISH versus PCR methods[J]. Laboratory Medicine,2012, 43(6):276-280.
[5] 周全. IL-6在鸡葡萄球菌性关节炎发病机制中的作用研究[D]. 武汉:华中农业大学,2005
[6] 朱晓平,范国雄,程金科,等.鸡葡萄球菌病发病机理的研究[J]. 畜牧兽医学报,1990,21(2):167-173.
[7] 刘文峰. 一起鸡痘病毒继发葡萄球菌感染病例的诊断[D].长春:吉林大学,2014.
[8] 朱国霞,王龙. 鸡大肠杆菌与葡萄球菌混合感染[J]. 中国兽医杂志,1998,24(11):27.
[9] 黄艳,王桂波,李世莲,等. 鸡新城疫、大肠杆菌与葡萄球菌混合感染的诊治[J]. 黑龙江畜牧兽医,2002,19(5):23.
[10] Chen J, Fang Q. Whole-genome sequencing analysis of methicillin-resistant Staphylococcus simulans causing surgical site infection[J]. Genome Announcements,2016,4(3):1-2.
[11] St?pień Pys'niak D, Wilczyński J, Marek A, et al. Staphylococcus simulans associated with endocarditis in broiler chickens[J]. Avian Pathology,2016,17(10):1-8.
[12] Yang H C,Chen S,David G W.et al.Characterization of multiple antimicrobial resistant Escherichia coli isolates from diseased chickens and swine in China[J].Journal of Clinical Mierobiology,2004,42(8):3483-3489.
[13] Murray B E. New aspects of antimicrobial resistance and resulting the therapeutic dilemmas[J].J Infect Dis,1991,163(6):1184-1194.
[14] 明双喜,刘艳,王沂蒙,等. 应用新型药剂降低动物性食品中抗生素残留[J]. 中国家禽,2008,30(6):32-35.
[15] 王瑞,魏源送. 畜禽粪便中残留四环素类抗生素和重金属的污染特征及其控制[J]. 农业环境科学学报,2013,32(9):1705-1719.
[16] 张慧敏,章明奎,顾国平. 浙北地区畜禽粪便和农田土壤中四环素类抗生素残留[J]. 生态与农村环境学报,2008,24(3):69-73.
[17] Blanco J E, Blanco M, Mora A, et al. Production of toxins(enterotoxins, verotoxins, and necrotoxins) and colicins by Escherichia coli strains isolated from septicemic and health chickens:Relationship with in vivo pathogenicity[J]. J Clin Microbiol, 1997, 35(11):2953-2957.
[18] Manges A R, Johnson J R. Food-borne origins of Escherichia coli causing extraintestinal infections[J].Clin Infect Dis 2012,55(5):712-719.
[19] 李殷. 葡萄球菌疫苗研制的进展与挑战[J].国外医学(免疫学分册),2003,26(4):179-183.
[20] 张晓艳,易绍琼,陈薇. 金黄色葡萄球菌疫苗的研究进展[J].微生物学免疫学进展,2009,37(2):74-78.
[21] 贺常亮,韦旭斌,付本懂,等. 治疗鸡大肠杆菌病的中药方剂筛选[J]. 中国兽医学报,2013,33(12):1917-1922.

Isolation and Identification of Pathogen ofEscherichia coli andStaphylococcus Mixed Infection of Chichen in Guizhou Province

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Wenjing, WEI Kaimin, ZHANG Shaoqiu, CHEN Yong. Effects of Walnut Green Husk and Its Extract on Intestinal Morphology,Mucosal Antioxidant Activity and Microbial Diversity of Yellow-feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2056-2065.
[2]	TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer Infection on the Microflora of Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2129-2139.
[3]	LI Xi, MAO Yangyi, LUO Huidi, ZHAO Peng, LI Jun, ZHANG Li, LI Chunyan, WANG Honghao. Effects of Dietary Crude Fiber Level on Rumen Microbial Composition and Diversity of Fattening Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1251-1263.
[4]	TIAN Qiufeng, ZHANG Hong, YIN Junyi, LIU Qiujin, ZHANG Jun, WANG Yan, QIN Pingwei, ZHU Qinghe, MIAO Yan, CHEN Nannan, ZHANG Shufen, DING Xinying, MA Shanshan, WANG Zhen. Isolation,Identification and Drug Resistance Analysis of a Strain of Staphylococcus sciuri from Dairy Cow [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1482-1488.
[5]	ZHONG Rui, HE Tingmei, BI Bo, YANG Xingtao, SHI Xiaogang, JIN Senlong, FENG Gang, PENG Guangneng. Isolation,Identification and Drug Resistance Analysis of Enterococcus faecalis from Raccoon [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1112-1120.
[6]	ZENG Weihuan, LIU Yingbo, LI Rong, XIAO Wangcheng, DUAN Gang, QIN Li, DAI Feiyan. A Effect of Walnut Green Husks Extract on Intestinal Microorganisms in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 612-619.
[7]	SHI Zhengyu, YI Yi, HAN Shuyu, LUO Fuguang, HUANG Jie, LU Jingdi, LI Ya. Isolation and Identification of Edwardsiella tarda in Pelteobagrus fulvidraco and Analysis of Its Pathogenicity [J]. , 2018, 45(3): 807-813.
[8]	SHI Zhengyu, YI Yi, HUANG Desheng, LUO Fuguang, HUANG Jie, LU Jingdi, LI Ya. Isolation, Identification and Pathogenicity of a Strain of Citrobacter freundii [J]. , 2018, 45(2): 479-485.
[9]	HU Dandan, GUO Tingting, LI Na, ZHANG Lili, XU Xiaofeng. Effect of Fructooligosaccharide on the Structure and Diversity of Rumen Bacterial Community in Dairy Cow by 16S rDNA Sequencing Technology [J]. , 2018, 45(10): 2761-2771.
[10]	LV Ji-zhou, ZHAO Li, ZHAO Yu-lin, HAN Xue-qing, WANG Hui-yu, WU Shao-qiang. Morphological and Molecular Biology Identification of Ornithodoros lahorensis [J]. , 2017, 44(4): 1149-1158.
[11]	WEI Yang-dao, YI Yi, LI Ya, SHI Zheng-yu, WU Shi-hua, LIANG Jian-rong. Isolation, Identification and Pathogenicity Analysis of Morganella morganii in Clariasfuscus [J]. , 2017, 44(1): 221-226.
[12]	WEI Yang-dao, YANG Jun, WEN Yan-hong, SI Tu-ling, LIU Xia, YI Yi. Isolation,Identification and Pathogenicity of Aeromonas veronii from Lepidorthosis Pathogenic Bacteria of Tilapia [J]. , 2016, 43(11): 3047-3052.
[13]	WANG Xiao-yan, YUAN Ting, LIAO Hong, LUO Yong-jiu, HE Ting-mei, WANG Cheng-dong, LIU Xiao-min, WU Hong-lin, PENG Guang-neng. Construction of the 16S rDNA Clone Library of Intestinal Flora of the Captive Elderly Giant Panda [J]. , 2015, 42(6): 1402-1408.
[14]	DUAN Hui-qin, WANG Li. Isolation,Identification and Drug Resistance Analysis of Staphylococcus sciuri of Corydoras [J]. , 2015, 42(5): 1288-1293.
[15]	FANG Guang-yuan, MAO Hui-hua, JIANG Jia-jin, GU Ya-feng, YAN Wen-mei, DAI Ding-zhen, GUO Yu-fei. Isolation,Identification and 16S rDNA Homology Analysis of Escherichia coli from Animals in Different Cases [J]. , 2015, 42(12): 3126-3132.