›› 2016, Vol. 43 ›› Issue (11): 3047-3052.doi: 10.16431/j.cnki.1671-7236.2016.11.035

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Isolation,Identification and Pathogenicity of Aeromonas veronii from Lepidorthosis Pathogenic Bacteria of Tilapia

WEI Yang-dao1,2,3, YANG Jun4, WEN Yan-hong4, SI Tu-ling4, LIU Xia4, YI Yi1,2,3   

  1. 1. College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China;
    2. Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China;
    3. Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Liuzhou 545006, China;
    4. Liuzhou Fishery Technical Extension Station, Liuzhou 545006, China
  • Received:2016-03-24 Online:2016-11-20 Published:2016-11-18

Abstract:

In order to study the pathogenicity of the pathogenic bacteria in the vertical scale disease of Tilapia,one strain of pathogenic bacteria was isolated from different parts of the diseased Tilapia,named as GXKJDX01.Then the methods of 16S rDNA gene sequence analysis,bacterial morphology observation,physiological and biochemical identification,drug sensitivity test and artificial infection test were used.The results showed that the pathogenic strain GXKJDX01 was Aeromonas veronii,strain GXKJDX01 was not sensitive to 5 medicines,including penicillin G,erythromycin,vancomycin,and had a strong pathogenic effect in yellow-head catfish meat,Tilapia,loach and Procypris merus.11 kinds of drugs,including of enrofloxacin,levofloxacin,ceftriaxone,could be effective to the prevention and control.This study is the first report on the case of the pathogenic bacteria of the lepidorthosis,which was the Aeromonas veronii,and the theoretical basis was provided for the effective prevention and control.

Key words: Tilapia; Aeromonas veronii; 16S rDNA; lepidorthosis

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