拉合尔钝缘蜱的形态学及分子生物学鉴定

doi:10.16431/j.cnki.1671-7236.2017.04.030

Abstract

Abstract:

This study was to establish a method for the identification of Ornithodoros lahorensis with molecular biology. The samples of Ornithodoros lahorensis were collected from sheep in Xinjiang region and the morphological characters were screened by stereo microscopy and electronic microscopy. Then the 16S rDNA,18S rDNA and cytochrome oxidase Ⅰ(COⅠ) of samples were amplified by PCR and subsequently sequenced. Then the analysis of genetic divergences and Neighbor-Joining (NJ) phylogenetic tree were carried out based on the sequences acquired from Ornithodoros lahorensis,combined with the reference sequences of GenBank database. In this study,the comprehensive and clear micromorphological traits of ovoid scutum,round fovea of Ornithodoros lahorensis were provided. Besides,the DNA sequences of Ornithodoros lahorensis were assembled together with sequences of Argasidae and form monophyletic clades in the 16S rDNA and COⅠ based Neighbor-Joining trees. According to the intraspecific K2P genetic distances of Ornithodoros lahorensis (0~0.3%),we determined that the molecular biology method based on 16S rDNA and COⅠ could rapidly and accurately identify the species of Ornithodoros lahorensis.

Key words: COⅠ; 16S rDNA; 18S rDNA; Ornithodoros lahorensis; morphological characters; molecular identification

CLC Number:

S852.7

LV Ji-zhou, ZHAO Li, ZHAO Yu-lin, HAN Xue-qing, WANG Hui-yu, WU Shao-qiang. Morphological and Molecular Biology Identification of Ornithodoros lahorensis[J]. , 2017, 44(4): 1149-1158.

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Morphological and Molecular Biology Identification of Ornithodoros lahorensis

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