2株猪繁殖与呼吸综合征病毒的致病性分析

doi:10.16431/j.cnki.1671-7236.2017.05.031

Abstract

Abstract:

Samples of sick pigs suspected of porcine reproductive and respiratory syndrome (PRRS) infection were collected from Hebei and Fujian provinces in China in 2016. Studies were carried out to investigate the variation characteristics of the isolated virus strains.The virus isolates were propagated in Marc-145 cells and caused syncytial cytopathic effect. RT-PCR and indirect fluorescent assay (IFA) results showed that the isolates were HP-PRRSV and hence designated as PRRSV CZ16A and NP16A, respectively. In order to study the pathogenicity of CZ16A and NP16A, the isolated strains were purified by plaque-isolation, and then inoculated to 60-days-old healthy pigs, which were negative in both of PRRSV infection and antibody. Clinical observation, histopathological changes and viremia detection were carried out to evaluate the pathogenicity of these two strains of PRRSV. Results showed that both isolates could replicate in pigs and cause a high body temperature, but these two strains of PRRSV isolates could not cause morbidity or mortality in experimental animals.Autopsy results also showed that NP16A could cause consolidation of the lung, lymph node enlargement in tested animals, suggesting that its virulence was a little stronger than CZ16A.

Key words: porcine reproductive and respiratory syndrome virus(PRRSV); isolation and identification; pathogenic analysis

CLC Number:

S851.3

LI Zhi, SUI Xiu-kun, WU Jing, XIN Ting, LI Ming, GAO Xin-tao, JIANG Yi-tong, HOU Shao-hua. Pathogenic Analysis of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2017, 44(5): 1477-1483.

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Pathogenic Analysis of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus

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