贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究

doi:10.16431/j.cnki.1671-7236.2016.12.032

Abstract

Abstract:

The aim of this study was to evaluate the polymorphism and evolution in royal chicken using the tandem repeat microsatellite LEI0258 located within the chicken major histocompatibility complex B region (MHC-B). Genomic DNA was extracted from forty blood samples of royal chicken and used to be genotyped and sequenced. Combining the published sequences,the analysis of allele and nucleotide polymorphism and median-joining network were carried out. A total of nine alleles ranging from 193 to 297 bp in 40 samples were found,five of which was novel for royal chicken. The observed heterozygosity,expected heterozygosity and polymorphic information content were 0.625,0.716 and 0.666,respectively. Three alleles were corresponding to six available serotypes. Alleles were classified into five clusters,based on the SNPs and indels found within the sequences flanking the repeats. The alleles of royal chicken existed in clades A and B,both of which were also found in Red junglefowl. The results suggested that royal chicken had a middle or high level of genetic diversity in microsatellite LEI0258,and that might originate from Red junglefowl.

Key words: royal chicken; polymorphism; major histocompatibility complex; microsatellite LEI0258; evolution; serotype

CLC Number:

S831.2

HUANG Xun-he, ZHANG Jin-feng, CHEN Jie-bo, YE Wei-qing, LI Wei-na, ZHONG Fu-sheng, DU Bing-wang. Genetic Diversity of Microsatellite LEI0258 in MHC-B Region of Royal Chicken[J]. , 2016, 43(12): 3300-3305.

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Genetic Diversity of Microsatellite LEI0258 in MHC-B Region of Royal Chicken

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