猪A群轮状病毒BJ株的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2016.12.033

Abstract

Abstract:

Rotavirus infections are a major cause of viral diarrheas in young animals and children. Isolation and identification of rotavirus make a contribution to epidemiological survey and molecular biology study.A strain of porcine rotavirus was isolated in MA104 cell cultures from the intestinal contents of piglets with diarrhea in Beijing.The virus was identified to be porcine rotavirus by immunochromatography strip test, Real-time RT-PCR, immunofluorescence test and sequencing analysis.According to the sequence analysis, the virus was classified as group A porcine rotavirus, the genotype of VP4, VP6 and VP7 genes belonged to P[13], I5 and G11, respectively.The virus was designated Rotavirus A pig/China/BJ/2015/G11P[13].

Key words: porcine rotavirus; isolation; identification

CLC Number:

S852.65⁺9.4

YUAN Lin, HAN Tao, NI Jian-qiang, KANG Wen-hua, WANG Bao-yue, LI Xiao-xia, CHEN Ya-na, ZHAI Xin-yan, SHEN Jian-zhong. Isolation and Identification of Porcine Group A Rotavirus BJ Strain[J]. , 2016, 43(12): 3306-3313.

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Isolation and Identification of Porcine Group A Rotavirus BJ Strain

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