金樱子总黄酮的含量测定及酶提工艺优化

doi:10.16431/j.cnki.1671-7236.2016.12.011

Abstract

Abstract:

To determine the content of total flavonoids from Rosa laevigata Michx. fruits, and optimize the process of cellulase-assisted extraction, the content of total flavonoids from Rosa laevigata Michx. fruits were determined with a double-wavelength spectrophotometry with detection wavelength were 500 nm and 580 nm as the reference, using rutin as a standard material, and the process of cellulase-assisted extraction designed by orthogonal test was optimized with the yield of flavonoids as the evaluation index, and the effects of extraction temperature, concentration of ethanol, cellulase dosage, ratio of liquid to material and other 4 factors on the content of total flavonoids were investigated. It showed a good linearity (r=0.9981) between absorbance difference(ΔA) and the rutin mass ranging of 19.90 to 398.00 μg, the standard curve equation was y=0.0008x-0.0047, and the yield of total flavonoids was 87.94 mg/g, the average recovery and RSD were 98.23% and 8.77%, respectively. And the optimumcellulase-assisted extraction conditions of total flavonoids from Rosa laevigata Michx. fruits by orthogonal array design were extraction temperature of 80℃, concentration of ethanol of 40%, cellulase dosage of 0.5%, ratio of liquid to material of 40:1, extraction time of 90 min, pH of 6.0, and extraction numbers of 2. Various factors affecting extracting yield were in a order of extraction temperature > cellulase dosage > ratio of liquid to material > concentration of ethanol. In this condition of extraction, yield of total flavonoids from Rosa laevigata Michx. fruits was (93.35±3.15) mg/g. Therefore, it was suggested that extraction of total flavonoids from Rosa laevigata Michx. fruits by cellulase-assisted extraction was feasible, and the yield of total flavonoids was higher and its conditions was mild.

Key words: Rosa laevigata Michx.fruits; total flavonoids; cellulase; orthogonal array design; double-wavelength spectrophotometry

CLC Number:

S859.3

SHAN Guang-qiang, CHEN Zhi-gang, XU Guang-ren, LEI Hong-yu, TAN Yu-qin, SU Jian-ming. Content Determination of Total Flavonoids from Rosa laevigata Michx. Fruits and Optimization of Cellulase-assisted Extraction[J]. , 2016, 43(12): 3156-3162.

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Content Determination of Total Flavonoids from Rosa laevigata Michx. Fruits and Optimization of Cellulase-assisted Extraction

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