产纤维素酶地衣芽孢杆菌的诱变选育及其产酶条件优化

doi:10.16431/j.cnki.1671-7236.2016.04.023

Abstract

Abstract: In order to improve the ability of producing cellulase of Bacillus lichenniformis, the mutational strain was screened after Bacillus lichenniformis LY02 was induced by UV.Then the best optimal enzyme-producing condition, inoculum size, temperature, incubation time, initial pH and metal ions were studied.The results showed that the cellulase activity of mutated strain was increased by 34.31%.And the best optimal ferment conditions were as follows:Inoculation concentration was 1.5%, cultivated temperature was 37℃, ferment time was 24 h, initial pH of medium was 5.0, and K⁺ and Ba²⁺ could promote the cellulase producing.These results were contributed to develop the strain to be applied in practice.

Key words: Bacillus licheniformis; cellulase; mutation; enzyme-producing condition; enzyme activity

CLC Number:

Q925

YU Zu-hua, DING Ke, HOU Kui, LI Yuan-xiao, LI Wang, LIU Yi-chen, CAO Ping-hua, JIA Yan-yan, CHENG Xiang-chao. Mutation Breeding and Optimization of Enzyme-producing Condition of Bacillus lichenniformis Producing Cellulase[J]. , 2016, 43(4): 1006-1011.

References

[1] 王荣.我国秸秆综合利用现状分析与发展对策[J].现代商业,2013,5:277.
[2] 马光,郭继平.米曲霉发酵玉米秸秆产纤维素酶饲料条件的优化[J].生物技术,2010,6:15-19.
[3] Li W,Huan X,Zhou Y,et al.Simultaneous cloning and expression of two cellulase genes from Bacillus subtilis newly isolated from Golden Takin (Budorcas taxicolor Bedfordi)[J].Biochemical and Biophysical Research Communications,2009,383(4):397-400.
[4] 屈二军,谢展,马孟星,等.高产纤维素酶的绿色木霉菌种的诱变和筛选[J].农业与生物技术学报,2011,10:1411-1412.
[5] 张漫莉,耿新伟,王梦婷,等.纤维素酶EGA基因在枯草芽孢杆菌中的表达及其产物性质研究[J].浙江理工大学学报,2013,30(3):390-393.
[6] 魏艳红.纤维素酶产生菌的分离鉴定及产酶条件优化[D].武汉:华中师范大学,2009.
[7] 祖彩霞,李志敏,叶勤.里氏木霉Trichoderma reesei产纤维素酶的发酵培养基碳氮源优化[J].生物加工过程,2011,9(4):6-10.
[8] Quang L T,Youngjae C,Abhijit K B,et al.Mutation of purD and purF genes further attenuates Brucella abortus strain RB51[J]. Microbial Pathogenesis,2015,79:1-7.
[9] Michelle Q C,Jacqueline W L,Steven H,et al.Natural rpoS mutations contribute to population heterogeneity in Escherichia coli O157:H7 strains linked to the 2006 US spinach-associated outbreak[J]. Food Microbiology,2014,44:108-118.
[10] 丁轲,余祖华,恒子钤,等.产纤维素酶芽孢杆菌的诱变和酶学性质研究[J].饲料研究,2012,9:38-41.
[11] 吕志伟,王瑾,张文会.地衣芽孢杆菌LCB-8的紫外诱变及优良菌株的选育[J].安徽农业科学,2011,39(32):19995-19996.
[12] 苏香萍,龚大春,曾晶,等.纤维素酶产生菌黑曲霉的选育及其产酶条件的研究[J].酿酒科技,2009,4:25-27.
[13] Yang Z,Kong F X,Shi X L,et al.Effects of UV-B radiation on microcystin production of a toxic strain of Microcystis aeruginosa and its competitiveness against a non-toxic strain[J].Journal of Hazardous Materials,2015,283:447-453.
[14] 方尚玲,杨丹丹,钱志伟,等.高产纤维素酶生产菌的筛选及诱变育种[J].食品与发酵科技,2010,46(1):13-17.
[15] 吴丹.高产纤维素酶菌株的分离鉴定、诱变选育及产酶条件的研究[D].南昌:南昌大学,2007.
[16] 高云航,张喜宏,王巍,等.牛粪中纤维素分解菌的分离鉴定及其产酶条件优化研究[J].中国畜牧兽医,2014,41(3):151-155.
[17] 奚晓琦,王加启,卜登攀,等.产蛋白酶和纤维素酶纳豆芽孢杆菌(B.Subtilis Natto)的筛选及其生物特性研究[J].中国畜牧兽医,2011,38(1):33-38.

Mutation Breeding and Optimization of Enzyme-producing Condition of Bacillus lichenniformis Producing Cellulase

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Yu, ZHAO Ruli, GAO Yuan. Analysis of Codon Preference in Camelus ferus Genome [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 429-439.
[2]	CHEN Xueying, WANG Huisheng, WU Jie, DENG Bo, DIAO Xinping. Effects of Dietary Bacillus licheniformis and Bacillus amyloliquefaciens on Growth Performance and Blood Indicators of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2547-2556.
[3]	ZHANG Yu, MA Xi, ZHAO Jinbiao, LU Lin. Optimization of Fermentation Conditions and Determination of Antibacterial Activity of Bacillus licheniformis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2812-2819.
[4]	CAI Rui, ZHANG Shuai, CUI Xinyu, XU Chuncheng. Effect of Adding High Temperature Period Compost on Degradation of Organic Components and Enzyme Activity of Sheep Manure Composting on Qinghai Tibet Plateau [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2831-2841.
[5]	WANG Jiaxin, HAN Yunsheng, CAI Hongying, WEN Zhiguo, XU Xin, ZHAO Zitao, MENG Kun, YANG Peilong. Effects of Bacillus licheniformis on Growth Performance, Serum Biochemical Indexes and Intestinal Morphology of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4593-4603.
[6]	LAN Qun, XIE Yingyu, CAO Jiacheng, XUE Li'e, LI Jiaquan, ZHAO Fanglu, LIN Ruiyi, FANG Shaoming, XIAO Tianfang. Effect of P-coumaric Acid on the Quality of Boar Semen Preserved at Room Temperature [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2928-2936.
[7]	HAN Chengxing, ZHANG Chengxin, LI Yong, CAI Xiaofang, LIU Peng, GUO Yanli, HAN Xiangmin. Study on Variations of Gastrointestinal Development, Enzyme Activity and Fermentation Parameters of Hu Lambs Pre- and Post-early Weaning [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4442-4450.
[8]	WANG Yu, SONG Ruigao, ZHAO Jianguo, WANG Yanfang. Efficient Site-directed Mutation of Porcine IGF2 Gene via Base Editors [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3427-3435.
[9]	LIU Yufu, DONG Hao, SUN Shijing, PENG Xiaowei, CHEN Ruiai, DING Jiabo, JIANG Hui. Construction of Brucella ClpS Gene Mutant Strain and Its Effect on Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3767-3773.
[10]	HU Rongbin, XING Xiaoyong, WU Xiaochun, ZHANG Yangyang, HE Jian, ZHANG Shengying, BAO Shijun. Prokaryotic Expression,Enzyme Activity Analysis and Subcellular Localization of Mycoplasma synoviae GDPD Gene [J]. , 2019, 46(8): 2228-2235.
[11]	CHEN Long, WU Xingli, LI Lijia, WEI Bingdong, YU Wei, YAN Xiaogang, GU Wei, XU Haiyan, LIU Chen, LIU Hongliang, ZHANG Fangyu. Optimization of Enzyme Production Conditions and Analysis of Enzymatic Properties of a High-yield Endocellulolytic Enzyme Bacillus velezensis [J]. , 2019, 46(5): 1353-1361.
[12]	ZHAO Mengdi, DI Lingfeng, TANG Zeyu, CAO Xue, LI Chengyun. Effects of Tannin and Feeding Cellulase on Rumen Microflora of Hu Sheep [J]. , 2019, 46(1): 112-122.
[13]	ZHANG Juan, MU Tong, ZHAO Ping, FENG Xiaofang, WU Jianping, GU Yaling. Bioinformatics Analysis of Structure and Function of PON1 Gene in Longdong Cattle [J]. , 2018, 45(7): 1750-1758.
[14]	WANG Yuanyuan, YAN Guo, LUO Cheng, QI Jiangjiao, TAN Jun, ZHOU Guangpu, ZHOU Yongshun, XU Jie, GAO Jianfeng. Construction and Identification of Yeast Surface Display Library of Sheep DRB1 Gene Exon 2 [J]. , 2018, 45(4): 850-857.
[15]	WEI Haiting, LIU Hanlu, LI Guangyu, SI Huazhe, LIU Xiaoying. Isolation, Identification and Determinations of Cellulose-degrading Bacteria from Sika Deer (Cervus nippon) Rumen [J]. , 2018, 45(4): 888-897.