响应面优化转透明颤菌血红蛋白基因里氏木霉液体发酵产纤维素酶的培养条件

doi:10.16431/j.cnki.1671-7236.2018.03.014

Abstract

Abstract:

In this study,the culture condition of Trichoderma reesei Tu6-VHb liquid fermentation to produce cellulases were optimized by response surface method and using filter paper activity(FPA) as the response value.First,three significant factors of enzyme production were screened by P-B test according to single factors’ result which were Tween-80 content,fermentation volume (250 mL triangular flask fermentation) and nitrogen source concentration.Then approached the maximum response area by the path of steepest ascent method.Finally,the response surface method was used to determine the interaction between each two main factors and optimum formula of culture medium.The results showed that the optimized fermentation condition in Tu6-VHb producing cellulase were that Tween-80 content was 0.39%,fermentation volume (250 mL triangular flask fermentation) was 61.32 mL and nitrogen source concentration was 0.87%.After fermentation,the cellulase activity produced by Tu6-VHb could be up to 51.72 U/mL,which close to the model prediction (51.94 U/mL),29.35% higher than that before optimization (39.984 U/mL),and 1.44 times of that of Trichoderma reesei Tu6 strain without Trichoderma reticum hemoglobin gene (35.904 U/mL).

Key words: Trichoderma reesei; cellulase; response surface method; liquid fermentation; Vhb

CLC Number:

Q939.9

YAN Lin, ZHONG Hongmei, ZUO Jie, CHEN Zhihui, LUO Youcai, CHEN Qiyue, WU Hong. Optimization of Culture Condition in Trichoderma reesei Tu6-VHb Which Transferred VHb Gene Cellulase Production by Surface Method[J]. , 2018, 45(3): 663-672.

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Optimization of Culture Condition in Trichoderma reesei Tu6-VHb Which Transferred VHb Gene Cellulase Production by Surface Method

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