细毛羊LAMB1基因外显子多态性及其与羊毛纤维直径的关联性分析

doi:10.16431/j.cnki.1671-7236.2016.09.027

Abstract

Abstract:

This study aimed to research the single nucleotide polymorphism (SNPs) of LAMB1 gene exon and its correlation with the fiber diameter in Fine-wool sheep.Based on DNA pools with the Re-sequencing technology to gain SNPs data,totally screened 20 SNPs of LAMB1 gene exon regions,at the same time,combining with direct sequencing method,PCR-SSCP and bioinformatics software to verify the accuracy of 10 missense mutations.The genetic effects of LAMB1 on fiber diameter at Xinjiang Kunes farm were analyzed by the GLM of SAS,totally 300 sheep.The results showed that 20 SNPs of LAMB1 gene were screened,which included 10 synonymous mutation SNPs and 10 non-synonymous mutation SNPs,after the validation,there were 7 missense mutation SNPs which led to the nature of protein change,3 SNPs were not mutated.LAMB1 protein became increasingly hydrophobic after mutating,predicting that was insoluble protein.TT genotype in the fiber diameter of the individual value was significantly higher than TC and CC genotypes on SNP5(P<0.05),GG and TT genotypes in the fiber diameter of the individual value was significantly higher than GT genotype on SNP9 (P<0.05).Although,all of the SNPs were completely detected by DNA pool with the Re-sequencing technology and minimized the false positives,it still need to be validated by direct sequencing.The results revealed that LAMB1 gene existed highly genetic diversity,mutated SNPs were unevenly distributed in exons,SNP5(rs159769941) and SNP9(rs159769901) could be considered as an effective genetic markers of Fine-wool sheep on fiber diameter.

Key words: DNA pool; Re-sequencing; LAMB1 gene; SNPs

CLC Number:

S813.3

ZHAO Bing-ru, HUANG Xi-xia, TIAN Ke-chuan, FU Xue-feng, XU Xin-ming, BAI Yan, TIAN Yue-zhen. Polymorphism of LAMB1 Gene and Its Association Analysis with the Fiber Diameter in Fine-wool Sheep[J]. , 2016, 43(9): 2401-2411.

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Polymorphism of LAMB1 Gene and Its Association Analysis with the Fiber Diameter in Fine-wool Sheep

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