哈萨克羊DRB1基因外显子3多态性及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.03.021

Abstract

Abstract:

This study was aimed to investigate the association between the polymorphism of lymphocyte antigen DRB1 gene exon 3 and the brucellosis susceptibility to Kazakh sheep.The polymorphism of DRB1 gene exon 3 in Kazakh sheep was analyzed using direct sequencing method of mixed DNA pooling combined with PCR products.The differences in gene frequency and genotype frequency of each SNP site were analyzed by chi-square test.Bioinformatics software was used to analyze the secondary structure of RNA,the secondary structure of protein and antigen epitope of the PCR amplification sequence.The sequencing result showed that 7 SNPs sites were detected in 282 bp DNA sequence,including T10C,C119T(Trp→Arg),G215C(Gln→Glu),A238G,T245G(Ser→Ala),G256A and C259T,there was no significant difference in gene frequency and genetype frequency between brucellosis case and control groups (P > 0.05).The further analysis showed that the mutation site all caused the change of the secondary structure of RNA and minimum free energy,each missense mutation did not cause the change of protein secondary structure and antigen epitope.The results showed that the 7 SNPs sites (T10C,C119T,G215C,A238G,T245G,G256A and C259T) of DRB1 gene exon 3 had no correlation with brucellosis susceptibility in Kazakh sheep.

Key words: DRB1 gene; DNA pooling; brucellosis; susceptibility; bioinformatics

CLC Number:

S813.3

WANG Yuanyuan, YAN Guo, LUO Cheng, QI Jiangjiao, ZHOU Guangpu, TAN Jun, GAO Jianfeng. Polymorphism and Bioinformatics Analysis of DRB1 Gene Exon 3 in Kazakh Sheep[J]. , 2018, 45(3): 721-729.

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Polymorphism and Bioinformatics Analysis of DRB1 Gene Exon 3 in Kazakh Sheep

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