长白猪prepro-orexin基因多态性及其与采食和生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.03.020

Abstract

Abstract:

In order to investigate the effects of prepro-orexin gene on feeding and growth traits of Landrace pigs,the polymorphisms of C62T and G426A sites of prepro-orexin gene in 132 Landrace pigs were detected using PCR product direct sequencing,the association of the two sites with feeding and growth traits during 30 to 100 kg body weight were analyzed.The results showed that the two sites of prepro-orexin gene were in Hardy-Weinberg equilibrium in Landrace pigs,and the frequency of wild-type alleles C and G were higher than those of mutant-type alleles T and A;In the four haplotypes and seven haplotype combinations,CG,TA haplotypes and the combinations CG/CG,TA/CG,TA/TA were the dominant types.The CC genotype of C62T,GG genotype of G426A and haplotype combination CG/CG could significantly decrease the number of visits per day (NVD),prolong the time spent on eating per visit (TPV) and increase the feed intake per visit (FIV)(P < 0.05),which the NVD,TPV and FIV of combination CG/CG were 1.22 times fewer,0.79 min longer and 32.18 g greater than those of combination TA/CG (P < 0.05),but there were no significant difference from combination TA/TA (P > 0.05).The TT genotype of C62T,AA genotype of G426A and haplotype combination TA/TA could significantly or extremely significantly shorten the days to 100 kg body weight (D100) and increase the average daily gain (ADG) (P < 0.05 or P < 0.01),which the D100 and ADG of the combination TA/TA were 6.23 d and 47.91 g/d shorter than those of combination CG/CG (P < 0.05;P < 0.01),6.69 d and 50.04 g/d higher than those of combination TA/CG (P < 0.05;P < 0.01).In Landrace pigs,the influences of C62T and G426A sites of prepro-orexin gene on the feeding and growth traits had obvious synergistic effects.

Key words: Landrace pig; prepro-orexin gene; single nucleotide polymorphism (SNP); feeding trait; growth trait

CLC Number:

S813.3

HU Wei, LI Mingli, ZHANG Yanlin, YAN Dawei, LI Zhongchang, DONG Xinxing, JI Chunlv, LAN Guoxiang, PAN Yuchun, LU Shaoxiong. Polymorphism of prepro-orexin Gene and Its Association Analysis with Feeding and Growth Traits in Landrace Pigs[J]. , 2018, 45(3): 712-720.

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Polymorphism of prepro-orexin Gene and Its Association Analysis with Feeding and Growth Traits in Landrace Pigs

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