奶牛源MRSA菌株的分离鉴定和耐药性分析

doi:10.16431/j.cnki.1671-7236.2016.07.031

Abstract

Abstract:

In this study,we collected 502 california mastitis test (CMT) positive milk samples from several cattle farms and then the bacteria were isolated and identified from them.After that,we used multiplex-PCR for screening out methicillin-resistant Staphylococcus aureus (MRSA).Then we investigated the MRSA's antibiotics susceptibility to 19 kinds of antibiotics by discdiffusion method.The results showed that 338 strains of Staphylococci including 89 strains of MRSA were detected out from the 452 milk samples with bacteria.Additionally,most MRSA showed high sensitivity to vancomycin,fosfomycin,chloramphenicol and clarithromycin,while resistant to penicillin,polymyxin,spectinomycin and ceftazidime.The results provided certain factual basis and method for guiding the use of antibiotics in bovine mastitis,rapid identification of MRSA strains and prevention or control of MRSA infection.

Key words: bovine mastitis; MRSA; multiplex-PCR; drug sensitivity

CLC Number:

S855

FENG Qing, LIN Wei-dong, ZHANG Da-shuai, DAI Di, LIAO Jun, WANG Xian, WANG Yi-yang, LIANG Xiang-huan, ZHANG Jun, QIN Ai-jian, JIN Wen-jie. Isolation, Indentification and Antibiotic Resistance Analysis of Methicillin-resistant Staphylococcus aureus (MRSA) from Raw Cow Milk[J]. , 2016, 43(7): 1862-1868.

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Isolation, Indentification and Antibiotic Resistance Analysis of Methicillin-resistant Staphylococcus aureus (MRSA) from Raw Cow Milk

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