新疆艾比湖鹅喉羚mtDNA D-Loop区序列多态性研究

doi:10.16431/j.cnki.1671-7236.2016.05.029

Abstract

Abstract: To investigate the genetic diversity and genetic data of Gazella subgutturosa from Xinjiang Ebinur lake National Wetland Nature Reserves, the Ebinur lake goose antelope 35 feces samples were analyzed.Using noninvasive DNA technology, the mitochondrial DNA D-Loop 878 bp fragment was successfully amplified.Sequences results for GenBank database BLAST comparison and Clustal W and DNASP software analysis.The results showed that the sequences of the above fragments were successfully amplified, total 55 polymorphic loci were detected, which were not found insert and deficiency.This study found 15 variable sites, including 14 transversion sites and 1 top transposition.The average A, T, C and G contents in D-Loop were 28.03%, 31.57%, 24.84% and 15.56%, respectively.The contents of A+T (59.6%) were higher than G+C (40.4%).In addition, a total of 16 mtDNA haploid type were detected, haploid type polymorphism (h) was 0.886 ±0.037, nucleotide diversity degree (π) was 0.037±0.01528.The results showed that Ebinur lake Gazelle subgutturosa maintained high variation in mitochondrial D-Loop sequences.

Key words: Ebinur lake; Gazelle subgutturosa; populations; mtDNA D-Loop; genetic diversity

CLC Number:

S813.3

TAJIGUL Turap, EZIZJAN Nabi, NING Li-qun, MAHMUT Halik. Genetic Diversity of Gazella subgutturosa Based on Mitochondrial DNA D-Loop Sequence in Xinjiang Ebinur Lake[J]. , 2016, 43(5): 1316-1322.

References

[1] Kingswood S C,Blank D A.Gazella subgutturosa[J].Mammalian Species,1996,518:1-10.
[2] 孙铭娟,高行宜,阿不力米提·阿不都卡迪尔,等.鹅喉羚(Gazella subgutturosa)研究动态[J].干旱区研究,2002,19(3):75-80.
[3] Haque M N,Smith T R.Reintroduction of Arabian sand gazelles Gazelle subguttrosa marica in Saudi Arabia[J].Boil Conserv,1996,76(2):203-207.
[4] 高行宜,姚军.新疆哈密盆地初冬鹅喉羚的地理分布与种群数量[J].干旱区地理,2006,29(2):213-218.
[5] 吕雪峰,阿布力孜·阿布力米提,宫平,等.鹅喉羚线粒体D-Loop和Cytb序列遗传多样性分析[J].生物技术,2015,25(2):165-184.
[6] Hayatgheib D,Karami M,Farahmand H,et al.Morpho-logical study and comparison of western and eastern goitered gazelle(Gazella subgutturosa:Gueldenstaedt,1780) populations in Iran[J].Int J Environ Res,2011,5(1):225-232.
[7] Xia C J,Xu W X,Yang W K,et al.Seasonal and sexual variation in vigilance behavior of goitered gazelle (Gazella subgutturosa) in western China[J].J Ethol,2011,29(3):443-451.
[8] Xu W X,Xia C J,Lin J,et al.Diet of Gazella subgutturosa (Guldenstaedt,1780) and food overlap with domestic sheep in Xinjiang,China[J].Folia Zool,2012,61(1):54-60.
[9] Blank D,Ruckstuhl K,Yang W.Influence of population density on group sizes in goitered gazelle (Gazella subgutturosa Guld,1780)[J].Eur J Wildl Res,2012,58(6):981-989.
[10] Murtskhvaladze M,Gurielidze Z,Kopaliani N,et al.Gene introgression betweenGazella subgutturosa and G.marica:Limitations of maternal inheritance analysis for species identification with conservation purposes[J].Acta Theriol,2012,57(4):383-386.
[11] 李莹,徐文轩,杨维康,等.卡拉麦里山有蹄类自然保护区鹅喉羚生境适宜性评价[J].兽类学报,2010,30(1):11-20.
[12] 徐文轩,乔建芳,刘伟,等.鹅喉羚生态生物学研究现状[J].生态学杂志,2008,27(2):257-262.
[13] 於建国,陈静波,多来提尼牙孜·依明,等.和田黑鸡mtDNA控制区D-Loop序列分析[J].中国畜牧兽医,2011,38(7):105-107.
[14] 敦松长.牦牛遗传多样性、起源驯化及分类学地位研究-线粒体DNA D-Loop序列分析[D].西宁:中国科学院西北高原生物研究所,2006.
[15] Sorokin P A,Soldatova N V,Lukarevskiy V S,et al.Genetic diversity and relations of the goitered gazelle (Gazella subgutturosa) groups from Uzbekistan,Turkmenistan,and Azerbaijan:Analysis of mitochondrial DNA[J].Biology Bulletin,2011,38(6):585-590.
[16] Bandelt H J,Forster P,Rohl A.Median-joining networks for inferring intraspecific phylogenies[J].Mol Biol Evol,1999,16(1):37-48.
[17] Tamura K,Stecher G,Peterson D,et al.Mega 6:Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution,2013,30(4):2725-2729.
[18] Tamura K,Peterson D,Peterson N,et al.Mega 5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28(10):2731-2739.
[19] Waits L,Paetkau D.Noninvasive genetic sampling tools for wildlife biologists:A review of applications and recommendations for accurate data collection[J].Journal of Wildlife Management, 2005,69(4):1419-1433.
[20] Waits L P.Using non-invasive genetic sampling to detect and estimate abundance of rarewild life species[J].Molecular Ecology,2004,10:249-256.
[21] 阿地力江·卡德尔,恩特马克,曹杰,等.普氏野马线粒体DNA D-Loop区序列多态性研究[J].生物技术,2008,18(4):4-7.
[22] Irwin D M,Kocher T D,Wilson A C.Evolution of the cytochrome b gene of mammals[J].Journal of Molecular Evolution.1991,32(2):128-144.
[23] 吴兰苏,杨爱珍,刘和,等.线粒体调控细胞凋亡的研究进展[J].中国农学报,2010,26(8):63-68.
[24] Zhou H,Li D Q,Zang Y G,et al.Study on mitochondrial DNA genetic diversity of Tibet an antelope[J].Hereditas,2006,28(3):299-305.
[25] Neigel J E,Avise J C.Application of a random walk model to geographic distributions of animal mitochondrial DNA variation[J].Genetics,1993,135(4):1209-1220.
[26] 艾萨江·图合提.基于线粒体DNA D-Loop区的新疆鹅喉羚种群遗传结构和遗传多态性研究[D].乌鲁木齐:新疆大学,2013.
[27] 艾孜孜江·乃比,夏米西丁·阿不都热依木,热木图拉·阿卜杜克热木,等.艾比湖国家级湿地自然保护区鹅喉羚种群数量与分布[J].四川动物,2015,3:447-452.

Genetic Diversity of Gazella subgutturosa Based on Mitochondrial DNA D-Loop Sequence in Xinjiang Ebinur Lake

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