4个主效基因与英系长白猪繁殖性能的相关性分析

doi:10.16431/j.cnki.1671-7236.2017.04.021

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1087-1094.doi: 10.16431/j.cnki.1671-7236.2017.04.021

4个主效基因与英系长白猪繁殖性能的相关性分析

胡慧艳¹, 贾青^1,2,3, 侯胜奎¹, 刘津¹, 赵思思¹, 李晓敏¹, 张伟峰⁴, 李书杨⁵, 王贵江⁶

1. 河北农业大学动物科技学院, 保定 071000;
2. 国家北方山区农业工程技术研究中心, 保定 071000;
3. 河北省山区农业工程技术研究中心, 保定 071000;
4. 河北工程大学生命科学与食品工程学院, 邯郸 056000;
5. 河北双鸽美丹畜牧科技有限公司, 晋州 052200;
6. 河北省畜牧良种工作站, 石家庄 050061

收稿日期:2016-10-24 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 贾青 E-mail:jiaqing@hebau.edu.cn
作者简介:胡慧艳(1989-),女,河北石家庄人,博士生,研究方向:动物遗传育种与繁殖,E-mail:huhuiyan315@163.com
基金资助:
河北省现代农业产业技术体系专项（HBCT2013070202）

The Association Analysis Between 4 Major Genes and Reproductive Traits in British Landrace Pigs

HU Hui-yan¹, JIA Qing^1,2,3, HOU Sheng-kui¹, LIU Jin¹, ZHAO Si-si¹, LI Xiao-min¹, ZHANG Wei-feng⁴, LI Shu-yang⁵, WANG Gui-jiang⁶

1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
2. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China;
3. Engineering Research Center for Agriculture in Hebei Mountainous Areas, Baoding 071000, China;
4. School of Life Science and Food Engineering, Engineering University of Hebei, Handan 056000, China;
5. Hebei Double Pigeon Meidan Animal Husbandry Technology Co., Ltd., Jinzhou 052200, China;
6. Animal Husbandry and Breeding Station of Hebei, Shijiazhuang 050061, China

Received:2016-10-24 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

为寻找有效的分子标记用于英系长白猪辅助育种进而提高其繁殖性能，在英系长白猪群（169头）中采用PCR-RFLP技术，进行雌激素受体（estrogen receptor，ESR）、视黄醛结合蛋白（retinol binding protein 4，RBP4）、瘦素（leptin，LEP）和促卵泡素亚基β（follicle-stimulating hormone β，FSHβ） 4种与繁殖性状相关基因的基因型频率检测，利用SPSS 19.0统计软件，采用最小二乘法将各基因不同基因型与总产仔数、产活仔数、断奶窝重、断奶头数进行了相关分析。结果显示，ESR、RBP4、LEP基因均以A为优势等位基因，FSHβ基因以B为优势等位基因，其中LEP、FSHβ基因多态位点符合Hardy-Weinberg平衡定律（P>0.05），而ESR、RBP4基因多态位点处于Hardy-Weinberg不平衡状态（P<0.01）；LEP基因在长白猪母猪中表现为BB基因型为有利基因型，其总产仔数、产活仔数、断奶头数分别比AB基因型高1.3219、1.6855、1.1710头（P<0.05）；FSHβ基因中AA基因型为不利基因型，BB基因型断奶窝重比AA基因型高5.1129 kg（P<0.05）；ESR、RBP4基因对长白母猪繁殖性能均无显著影响（P>0.05），而LEP、FSHβ基因可能作为有价值的候选基因应用于英系长白猪的分子标记辅助选择。

关键词: 英系长白猪; 主效基因; 繁殖性状; 关联分析

Abstract:

The purpose of this study was to identify candidate genes associated with reproduction traits and explore the available molecular marker in British Landrace pig breed. The PCR-RFLP technique was applied to analyze the distribution of estrogen receptor (ESR), retinol binding protein 4 (RBP4), leptin (LEP) and follicle-stimulating hormone β (FSHβ) genes in British Landrace pigs. The genetic effects of ESR, RBP4, LEP and FSHβ on the reproductive traits included total number born, number born alive, weaning weight of litter, weaning piglet, which were analyzed by using least square method of SPSS 19.0 software. This results demonstrated that A was dominant allele in ESR, RBP4, LEP genes, and B was dominant allele in FSHβ gene. The result of χ² test showed that the polymorphic sites of LEP, FSHβ genes were fit with Hardy-Weinberg equilibrium (P>0.05), while this site of ESR and RBP4 were not fit with Hardy-Weinberg equilibrium (P<0.01). In addition, the statistical analysis indicated that the total number born, number born alive and number of alive weaning litter with BB genotype of LEP were higher than AA genotype, which were significantly increased by 1.3219, 1.6855 and 1.1710 (P<0.05), respectively;The FSHβ loci had a remarkable effect on reproductive performance, the average weaning weight with BB genotype was significantly higher than AA genotype, which was significantly increased by 5.1129 kg (P<0.05). The ESR and RBP4 loci had no significant effect on reproductive traits (P>0.05), while the LEP and FSHβ genes were valuable candidates for possible marker-assisted selection in British Landrace pigs population.

Key words: British Landrace pig; major gene; reproductive traits; correlation analysis

中图分类号:

S813.3

胡慧艳, 贾青, 侯胜奎, 刘津, 赵思思, 李晓敏, 张伟峰, 李书杨, 王贵江. 4个主效基因与英系长白猪繁殖性能的相关性分析[J]. 《中国畜牧兽医》, 2017, 44(4): 1087-1094.

HU Hui-yan, JIA Qing, HOU Sheng-kui, LIU Jin, ZHAO Si-si, LI Xiao-min, ZHANG Wei-feng, LI Shu-yang, WANG Gui-jiang. The Association Analysis Between 4 Major Genes and Reproductive Traits in British Landrace Pigs[J]. , 2017, 44(4): 1087-1094.

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4个主效基因与英系长白猪繁殖性能的相关性分析

The Association Analysis Between 4 Major Genes and Reproductive Traits in British Landrace Pigs

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