›› 2007, Vol. 34 ›› Issue (11): 20-22.

• 动物营养与饲料科学 • 上一篇    下一篇

维生素E缺乏对雏鸡淋巴细胞膜抗氧化及膜流动性的影响

王奔,李艳飞,封家旺,张金龙   

  1. 东北农业大学动物医学院,哈尔滨 150030
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-11-20 发布日期:2007-11-20

The Effect of Vitamin E Deficiency on Antioxidation and Fluidity of Lymphocyte Membrane in Chicken

WANG Ben,LI Yanfei,FENG Jiawang,ZHANG Jinlong   

  1. College of Animal Medicine,Northeast Agricultural University,Harbin 150030,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-20 Published:2007-11-20

摘要: 用低维生素E(VE)和添加不饱和脂肪酸的日粮饲喂雏鸡,建立VE缺乏雏鸡模型。分离全血淋巴细胞并制备细胞膜,检测淋巴细胞膜超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量;采用荧光偏振法检测淋巴细胞膜流动性。结果表明,VE缺乏雏鸡淋巴细胞膜SOD和GSH-Px活力及膜流动性低于对照组,MDA含量高于对照组,组间差异显著或极显著 (P<0.05、P<0.01)。说明VE对维持雏鸡淋巴细胞膜功能起重要作用。

关键词: 维生素E; 雏鸡; 淋巴细胞膜; 抗氧化; 膜流动性

Abstract: The activity of SOD and GSHPx and the level of MDA of lymphocyte membrane in the modle chicken with Vitamin E (VE)deficiency that produced by using low content of VE feedstuff and supplying unsaturated fatty acid in the diet were detected by Test Kit;the membrane fluidity of lymphocyte in the same modle chicken was detected through fluorescence polarization. The results showed that activity of SOD and GSH-Px and fluidity of lymphocyte membrane were significantly lower in experimental chicken than control ones(P<0.05,P<0.01);the MDA content were significantly higher than control ones (P<0.05).The results indicated that VE plays an important role in maintaining the function of lymphocyte membrane in chicken. 

Key words: VE; chicken; lymphocyte membrane; antioxidation; membrane fluidity

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