microRNA-21和TGFBI基因在猪骨骼肌生长发育中的表达分析

›› 2014, Vol. 41 ›› Issue (11): 191-197.

microRNA-21和TGFBI基因在猪骨骼肌生长发育中的表达分析

朱师云¹, 谢炳坤², 梁如意¹, 李奎¹, 唐中林¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 广西畜牧研究所, 广西南宁 530001

收稿日期:2014-04-09 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 唐中林 E-mail:zhonglinqy_99@sina.com
作者简介:朱师云(1989-),男,浙江人,硕士,研究方向:动物遗传育种与繁殖。
基金资助:
转基因生物新品种培育重大专项(2013ZX08009-001);国家自然科学基金(31171192);中国农业科学院科技创新工程(ASTIP-IAS05);广西家畜遗传改良重点实验室培育基地开放课题(桂牧研科2013GXKFKT-09)。

Expression Analysis of microRNA-21 and TGFBI Genes in Skeletal Muscle of Pigs at Different Development Stages

ZHU Shi-yun¹, XIE Bing-kun², LIANG Ru-yi¹, LI Kui¹, TANG Zhong-lin¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Guangxi Institute of Animal Science, Nanning 530001, China

Received:2014-04-09 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： 试验旨在研究microRNA-21(miR-21)和转化生长因子β诱导(TGFBI)基因在长白猪骨骼肌中的表达相关性。采用实时荧光定量PCR(qPCR)方法分析了miR-21和TGFBI基因在长白成年猪中的组织表达谱,同时比较分析了其在长白猪不同发育阶段(出生前和出生后共28个发育点)背最长肌中的表达相关性。结果表明,miR-21在长白成年猪的各个组织中均表达,且分布相对平衡,在不同发育阶段的背最长肌中呈现波浪式的表达趋势;TGFBI基因在长白猪胚胎期的背最长肌中高表达,在整个背最长肌发育过程中呈现出不同的表达丰度。相关性分析表明,在胚胎期骨骼肌发育过程中,miR-21和TGFBI表达之间为显著的负相关,TGFBI可能是miR-21的调控靶标基因。

关键词: miR-21; TGFBI; 骨骼肌; 发育; 猪

Abstract: The aim of this study was to investigate the correlativity between expression of microRNA-21 (miR-21) and transforming growth factor beta-induced (TGFBI) in skeletal muscles of Landrace pigs. Comparative analysis was conducted between miR-21 and TGFBI in different tissues and in skeletal muscles at 28 developmental stages by quantitative PCR (qPCR). The results showed that the miR-21 was expressed without specificity in different tissues and fluctuated in skeletal muscles at developmental stages; TGFBI was expressed highly in skeletal muscles at embryonic periods and has different expression levels during developmental periods of skeletal muscles. The correlativity analysis showed that there was significantly negative correlativity between miR-21 and TGFBI in embryonic period of skeletal muscle development, which indicated that TGFBI was regulated by miR-21 in pig skeletal muscle development.

Key words: miR-21; TGFBI; skeletal muscle; development; pig

中图分类号:

Q786

朱师云, 谢炳坤, 梁如意, 李奎, 唐中林. microRNA-21和TGFBI基因在猪骨骼肌生长发育中的表达分析[J]. , 2014, 41(11): 191-197.

ZHU Shi-yun, XIE Bing-kun, LIANG Ru-yi, LI Kui, TANG Zhong-lin. Expression Analysis of microRNA-21 and TGFBI Genes in Skeletal Muscle of Pigs at Different Development Stages[J]. , 2014, 41(11): 191-197.

参考文献

1 梁如意. microRNA-21对猪骨骼肌生长发育调控的研究[D].南京:南京农业大学,2011.
2 Asangani I A, Rasheed S, Nikolova D A, et al. microRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer[J]. Oncogene, 2008, 27(15): 2128～2136.
3 Ashmore C R, Addis P B, Doerr L. Development of muscle fibers in the fetal pig[J]. J Anim Sci, 1973, 36(6): 1088～1093.
4 Bae J, Lee S, Kim J, et al. βig-h3 supports keratinocyte adhesion, migration, and proliferation through α3β1 integrin[J]. Biochem Bioph Res Co, 2002, 294(5): 940～948.
5 Betel D, Wilson M, Gabow A, et al. The microRNA. org resource: Targets and expression[J]. Nucleic Acids Res, 2008, 36(suppl 1): D149～D153.
6 Bushati N, Cohen S M. microRNA functions[J]. Annu Rev Cell Dev Biol, 2007, 23: 175～205.
7 Callis T E, Chen J, Wang D. microRNAs in skeletal and cardiac muscle development[J]. Dna Cell Biol, 2007, 26(4): 219～225.
8 Davoli R, Braglia S, Russo V, et al. Expression profiling of functional genes in prenatal skeletal muscle tissue in Duroc and Pietrain pigs[J]. J Anim Breed Genet, 2011, 128(1): 15～27.
9 Hayashi T, Koyama N, Azuma Y, et al. Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro[J]. Dev Biol, 2011, 352(2): 299～307.
10 Hei T K, Zhao Y, Zhou H, et al. Mechanism of radiation carcinogenesis: Role of the TGFBI gene and the inflammatory signaling cascade[J]. Adv Exp Med Biol,2011,720: 163～170.
11 Hou X, Tang Z, Liu H, et al. Discovery of microRNAs associated with myogenesis by deep sequencing of serial developmental skeletal muscles in pigs[J]. PLoS One, 2012, 7(12): e52123.
12 Iorio M V, Ferracin M, Liu C, et al. microRNA gene expression deregulation in human breast cancer[J]. Cancer Res, 2005, 65(16): 7065～7070.
13 Kloosterman W P, Plasterk R H. The diverse functions of microRNAs in animal development and disease[J]. Dev Cell, 2006, 11(4): 441～450.
14 Liu C, Li B, Cheng Y, et al. MiR-21 plays an important role in radiation induced carcinogenesis in BALB/c mice by directly targeting the tumor suppressor gene βig-h3[J]. Int J Biol Sci, 2011, 7(3): 347～363.
15 Lu J, Getz G, Miska E A, et al. microRNA expression profiles classify human cancers[J]. Nature, 2005, 435(7043): 834～838.
16 Lytle J R, Yario T A, Steitz J A. Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5' UTR as in the 3' UTR[J]. Proceedings of the National Academy of Sciences, 2007, 104(23): 9667～9672.
17 Mccarthy J J, Esser K A, Andrade F H. microRNA-206 is overexpressed in the diaphragm but not the hindlimb muscle of mdx mouse[J]. Am J Physiol Cell Physiol, 2007, 293(1): C451～C457.
18 Meng F, Henson R, Wehbe J H, et al. microRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer[J]. Gastroenterology, 2007, 133(2): 647～658.
19 Papagiannakopoulos T, Shapiro A, Kosik K S. microRNA-21 targets a network of key tumor-suppressive pathways in glioblastoma cells[J]. Cancer Res, 2008, 68(19): 8164～8172.
20 Potthoff M J, Olson E N. MEF2: A central regulator of diverse developmental programs[J]. Development, 2007, 134(23): 4131～4140.
21 Sarkar J, Gou D, Turaka P, et al. microRNA-21 plays a role in hypoxia-mediated pulmonary artery smooth muscle cell proliferation and migration[J]. Am J Physiol Lung C, 2010, 299(6): L861.
22 Sayed D, Hong C, Chen I, et al. microRNAs play an essential role in the development of cardiac hypertrophy[J]. Circ Res, 2007, 100(3): 416～424.
23 Shenouda S K, Alahari S K. microRNA function in cancer: Oncogene or a tumor suppressor[J]. Cancer Metast Rev, 2009, 28(3～4): 369～378.
24 Skonier J, Neubauer M, Madisen L, et al. cDNA cloning and sequence analysis of βig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-β[J]. Dna Cell Biol, 1992, 11(7): 511～522.
25 Tao J, Lu Q, Wu D, et al. microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells[J]. Oncol Rep, 2011, 25(6): 1721～1729.
26 Wang J Y, Gao Y B, Zhang N, et al. Tongxinluo ameliorates renal structure and function by regulating miR-21-induced epithelial-mesenchymal transition in diabetic nephropathy[J]. American Journal of Physiology Renal Physiology, 2014,306(5):F486～F495.
27 Wigmore P M, Stickland N C. Muscle development in large and small pig fetuses[J]. J Anat, 1983, 137(Pt 2): 235.
28 Williams A H, Liu N, van Rooij E, et al. microRNA control of muscle development and disease[J]. Curr Opin Cell Biol, 2009, 21(3): 461～469.
29 Yang Y, Li Y, Liang R, et al. Dynamic expression of microRNA-127 during porcine prenatal and postnatal skeletal muscle development[J]. Journal of Integrative Agriculture, 2013,8(4): 1221～1234.
30 Zhang Z, Li Z, Gao C, et al. miR-21 plays a pivotal role in gastric cancer pathogenesis and progression[J]. Lab Invest, 2008, 88(12): 1358～1366.
31 Zhang J, Ying Z, Tang Z, et al. microRNA-148a promotes myogenic differentiation by targeting the ROCK1 gene[J]. J Biol Chem, 2012, 287(25): 21093～21101.
32 Zhao S, Zhang J, Hou X, et al. OLFML3 expression is decreased during prenatal muscle development and regulated by microRNA-155 in pigs[J]. International Journal of Biological Sciences, 2012, 8(4): 459～469.
33 Zhu S, Si M, Wu H, et al. microRNA-21 targets the tumor suppressor gene tropomyosin 1 (TPM1)[J]. J Biol Chem, 2007, 282(19): 14328～14336.

[1]	陈楷文, 赵云娇, 王磊, 张剑搏, 马玉红, 吴国芳. 不同氧浓度下猪睾丸间质细胞转录组分析[J]. 中国畜牧兽医, 2023, 50(6): 2185-2195.
[2]	韦明邦, 边巴琼达, 肖青青, 段梦琪, 强巴央宗, 商鹏. 藏猪CDKN1B基因克隆、生物信息学及表达分析[J]. 中国畜牧兽医, 2023, 50(6): 2196-2206.
[3]	杨瑰桃, 马继登, 李学伟, 葛良鹏, 张进威. 短链脂肪酸调控骨骼肌生理功能研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2286-2295.
[4]	张方玮, 张琪, 雷亮亮, 孙武胜, 张迪, 张云鹏, 张净搏, 王修权, 张晶, 张树敏. 松辽黑猪HBEGF基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2023, 50(6): 2370-2379.
[5]	孙守湖, 陈志飞, 郭智轩, 俞婷, 王修武, 李松倍, 魏文康, 贺东生. 3种猪冠状病毒N基因密码子偏好性及宿主适应机制研究[J]. 中国畜牧兽医, 2023, 50(6): 2403-2413.
[6]	李洁, 陈楚雯, 赵瑞鹏, 刘媛, 李志雄. lncRNA在畜禽肌肉发育中的研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2427-2438.
[7]	曹琪, 金清, 袁意, 冯逸雪, 孔丹妮, 陈翔鸿, 吴斌, 宋文博, 汤细彪. 规模化猪场常见呼吸道细菌性病原的流行病学调查[J]. 中国畜牧兽医, 2023, 50(6): 2439-2449.
[8]	史秋颖, 段宝敏, 李颖, 牛登云, 耿晨阳, 周佳伟, 王蕊, 冯敬敬. 2020—2021年中国部分地区猪流行性腹泻病毒S1基因遗传变异分析[J]. 中国畜牧兽医, 2023, 50(6): 2468-2478.
[9]	欧云文, 潘琴, 汪洋, 代军飞, 任绍科, 张洋, 张杰. 猪细小病毒6型ORF2基因的截短表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2487-2495.
[10]	片慧芳, 杜旭彬, 李妍, 张雨辰, 刘斐, 虞德兵. 海兰褐壳蛋鸡小黄卵泡颗粒细胞全长转录组测序分析[J]. 中国畜牧兽医, 2023, 50(5): 1754-1763.
[11]	武志娟, 柴志欣, 王吉坤, 王嘉博, 钟金城, 信金伟. 牦牛骨骼肌中特异基质金属蛋白酶及其功能相关基因的表达分析[J]. 中国畜牧兽医, 2023, 50(5): 1774-1784.
[12]	潘鹏丞, 雷宗全, 李显, 胡湘云, 秦谦涛, 覃兆鲜, 关志惠, 陈宝剑, 谢炳坤. 陆川猪MYL2基因生物信息学分析、真核表达载体构建及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1796-1806.
[13]	欧阳坤, 魏立民, 孙瑞萍, 解娅立, 齐琪, 李想, 刘圈炜, 晁哲, 张永亮, 陈婷. 姜黄素对海南屯昌猪生长性能、饲料养分表观消化率和血清生化指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1867-1875.
[14]	吴静璇, 孙征, 马雪莲, 张丽阳, 廖秀冬, 罗绪刚, 李素芬, 吕林. 中国畜禽饲料资源中镁含量的调查研究[J]. 中国畜牧兽医, 2023, 50(5): 1888-1898.
[15]	黎明国, 李清春, 陈鑫, 卢世豪, 何凡, 祁梦凡, 张化鹏, 任玉军, 张庆泽, 符彬彬, 徐梦思, 艾子凯, 闫坤, 冯赟, 华再东, 黄涛, 毕延震. TET去甲基化酶对猪卵母细胞发育的影响[J]. 中国畜牧兽医, 2023, 50(5): 1907-1917.