›› 2012, Vol. 39 ›› Issue (5): 145-147.

• 遗传繁育 • 上一篇    下一篇

策勒黑羊BMPR-IB基因多态性研究

代金平1, 刘武军1, 米日尼萨汗·库尔班2, 蔡高占1, 邵勇钢1   

  1. 1. 新疆农业大学动物科学学院, 新疆乌鲁木齐 830052;2. 新疆和田策勒县畜牧兽医站, 新疆和田 848300
  • 收稿日期:2011-12-22 修回日期:1900-01-01 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 邵勇钢

Study on the Polymorphism of BMPR-IB Gene in Xinjiang Cele Black Sheep

DAI Jing-ping1, LIU Wu-jun1, Mirinisahan Kuerban2, CAI Gao-zhan1, SHAO Yong-gang1   

  1. 1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;2. Cele Station of Animal Husbandry and Veterinary Medicine, Hetian, 848300, China
  • Received:2011-12-22 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20

摘要: 以影响布鲁拉美利奴羊繁殖力的骨形态发生蛋白受体-IB (bone morpho-genetic protein receptor-IB, BMPR-IB) 基因为候选基因,采用PCR-RFLP方法对策勒黑羊中BMPR-IB基因的多态性进行了研究。结果表明,在随机选取的100只策勒黑羊中检测到BB、B+和++ 3种基因型,其中BB、B+、++基因型频率分别为0.10、0.43和0.47,B基因频率为0.315,+基因频率为0.685,处于Hardy-Weinberg平衡状态(P>0.05)。推测BMPR-IB基因可能是影响策勒黑羊多羔性状的候选基因之一。

关键词: 策勒黑羊; BMPR-IB基因; PCR-RFLP; 多态性

Abstract: PCR-RFLP method was used to detect the polymorphism of BMPR-IB gene which controled the fecundity of Booroola Merino as a candidate gene on the fecundity of Cele Black sheep. The results showed that BMPR-IB gene had polymorphism in 100 Cele Black Sheep which were selected randomly. Three genotypes were found in Cele Black sheep, the genotypes frequencies of BB, B+ and ++ were 0.10, 0.43 and 0.47, the allelic frequencies of B and + were 0.315 and 0.685, and the genotype distribution was in agreement with Hardy-Weinberg equilibrium (P>0.05). This showed that BMPR-IB gene is probably one of the candidate genes with affecting the fecundity of Cele Black sheep.

Key words: Cele Black sheep; BMPR-IB gene; PCR-RFLP; polymorphism

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