柯乐猪MMP2基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.03.026

中国畜牧兽医 ›› 2024, Vol. 51 ›› Issue (3): 1142-1150.doi: 10.16431/j.cnki.1671-7236.2024.03.026

• 遗传繁育 • 上一篇

柯乐猪MMP2基因多态性及其与繁殖性状的关联分析

向进¹, 杨齐心², 罗华伦⁴, 洪亚婷¹, 郭小江³, 杨红文², 李维², 王春源¹, 吴燕¹, 张依裕¹

1. 贵州大学动物科学学院, 高原山地动物遗传育种与繁殖教育部重点实验室, 贵州省动物遗传育种与繁殖重点实验室, 贵州大学香猪研究所, 贵阳 550025;
2. 贵州省畜禽遗传资源管理站, 贵阳 550001;
3. 贵州省草地技术试验推广站, 贵阳 550001;
4. 黔西南州农业农村局, 兴义 562400

收稿日期:2023-09-13 发布日期:2024-02-27
通讯作者: 张依裕 E-mail:zyy8yyc@163.com
作者简介:向进,E-mail:1911005146@qq.com。
基金资助:
贵州省科技支撑计划(黔科合支撑[2021]一般147);贵州省科技计划项目(黔科合平台人才[2021]5630号);贵州省生猪产业发展项目(黔财农[2021]157号、黔财农[2022]183号);贵州省种业发展项目(黔农计财[2022]10号);贵州省生猪产业技术体系建设(GZSZCYJSTX-03);国家重点研发计划子课题(2022YFD1100308-01)

Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs

XIANG Jin¹, YANG Qixin², LUO Hualun⁴, HONG Yating¹, GUO Xiaojiang³, YANG Hongwen², LI Wei², WANG Chunyuan¹, WU Yan¹, ZHANG Yiyu¹

1. Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Xiang Pig Research Institute of Guizhou University, College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Guizhou Provincial Animal Disease Prevention and Control Center, Guiyang 550001, China;
3. Guizhou Grassland Technology Test and Extension Station, Guiyang 550001, China;
4. Agricultural and Rural Bureau of Qianxinan Prefecture, Xingyi 562400, China

Received:2023-09-13 Published:2024-02-27

摘要/Abstract

摘要： 【目的】探究基质金属蛋白酶2(matrix metallopeptidase 2,MMP2)基因单核苷酸多态性(single nucleotide polymorphisms,SNP)及其与繁殖性状的相关性,筛选与柯乐猪繁殖性状相关的遗传标记。【方法】试验选择212头健康的经产柯乐母猪,采用直接测序法筛选柯乐猪MMP2基因SNP位点,利用RNAfold在线软件预测MMP2基因不同单倍型mRNA二级结构,并通过SPSS 22.0软件分析MMP2基因SNP位点与柯乐猪繁殖性状的相关性。【结果】在柯乐猪MMP2基因第2外显子中检测到1个SNP位点(g.30062403 C＞T),在第4外显子中检测到2个SNPs位点(g.30065309 C＞T和g.30065312 C＞T),均存在3种基因型(CC、CT和CT)。遗传特性分析结果显示,g.30062403 C＞T位点为低度多态,g.30065309 C＞T和g.30065312 C＞T位点属于中度多态。卡方适合性检验结果显示,g.30062403 C＞T位点显著偏离Hardy-Weinberg平衡(P＜0.05),另2个位点均符合Hardy-Weinberg平衡(P＞0.05)。连锁不平衡分析结果显示,g.30065309 C＞T和g.30065312 C＞T位点之间存在强连锁不平衡。单倍型mRNA二级结构分析显示,单倍型H1的mRNA二级结构与原序列相同,其他3种单倍型均导致mRNA二级结构和最小自由能发生变化。关联分析结果显示,g.30062403 C＞T位点TT基因型个体的窝产活仔数显著高于CC基因型,断奶窝重显著高于CT基因型(P＜0.05);g.30065309 C＞T位点CC基因型个体的断奶仔数显著高于TT基因型(P<0.05);g.30065312 C＞T位点TT基因型个体的窝产活仔数、断奶仔数均显著高于CT基因型(P＜0.05)。双倍型H2H2为窝产仔数、窝产活仔数、断奶仔数、断奶窝重的优势双倍型,H1H4为初生窝重的优势双倍型。【结论】柯乐猪MMP2基因3个SNPs位点与其窝产仔数、窝产活仔数、初生窝重、断奶仔数及断奶窝重均存在显著关联性,可作为分子育种遗传标记。

关键词: 柯乐猪; MMP2基因; 多态性; 繁殖性状; 关联分析

Abstract: 【Objective】 The experiment was conducted to explore single nucleotide polymorphisms (SNP) of matrix metallopeptidase 2 (MMP2) gene and its correlation with reproductive traits,and to screen genetic markers related to reproductive traits of Kele pigs.【Method】 A total of 212 healthy partriparous Kele sows were selected and the SNP of MMP2 gene were screened by direct sequencing.Using RNAfold online software to predict the secondary mRNA structure of different haplotypes of MMP2 gene.SPSS 22.0 software was used to analyze the correlation between MMP2 gene SNP and reproductive traits of Kele pigs.【Result】 1 SNP (g.30062403 C＞T) was detected in exon 2 and 2 SNPs (g.30065309 C＞T and g.30065312 C＞T) were detected in exon 4 of MMP2 gene,all with 3 genotypes (CC,CT and CT).The results of genetic analysis showed that g.30062403 C＞T was a low polymorphism,and g.30065309 C＞T and g.30065312 C＞T were moderate polymorphisms.Chi-square fitness test results showed that g.30062403 C＞T significantly deviated from Hardy-Weinberg equilibrium (P<0.05),and the other 2 SNPs were in Hardy-Weinberg equilibrium (P＞0.05).Linkage disequilibrium analysis showed that there was strong linkage disequilibrium between g.30065309 C＞T and g.30065312 C＞T.The analysis of haplotype mRNA secondary structure showed that the mRNA secondary structure of haplotype H1 was the same as the original sequence,and the other 3 haplotypes all caused changes in the mRNA secondary structure and minimum free energy.The results of association analysis showed that the litter number of TT genotype at g.30062403 C＞T was significantly higher than that of CC genotype,and the weaning litter weight was significantly higher than that of CT genotype (P<0.05).The number of weaned piglets of CC genotype at g.30065309 C＞T was significantly higher than that of TT genotype (P<0.05). The number of live litter and weaned piglets of TT genotype at g.30065312 C＞T was significantly higher than that of CT genotype (P<0.05).The diplotype H2H2 was the dominant diplotype of litter number,litter born alive,weaned piglets and weaning litter weight,while H1H4 was the dominant diplotype of litter weight at birth.【Conclusion】 There was a significant correlation between of MMP2 gene 3 SNPs and litter number,litter born alive,litter weight at birth,weaned piglets and litter weight at weaning,which could be used as genetic markers for molecular breeding.

Key words: Kele pigs; MMP2 gene; polymorphism; reproductive traits; association analysis

中图分类号:

S813.3

向进, 杨齐心, 罗华伦, 洪亚婷, 郭小江, 杨红文, 李维, 王春源, 吴燕, 张依裕. 柯乐猪MMP2基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2024, 51(3): 1142-1150.

XIANG Jin, YANG Qixin, LUO Hualun, HONG Yating, GUO Xiaojiang, YANG Hongwen, LI Wei, WANG Chunyuan, WU Yan, ZHANG Yiyu. Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1142-1150.

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柯乐猪MMP2基因多态性及其与繁殖性状的关联分析

Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs

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