绵羊MSTN基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.07.017

摘要/Abstract

摘要： 【目的】探讨绵羊肌肉生长抑制素（myostatin，MSTN）基因多态性及其对绵羊肉质性状的影响，以期为绵羊肉用性能的选育提供有效的遗传分子标记。【方法】利用液相捕获测序技术获取MSTN基因在杜泊羊（D）、滩羊（T）和小尾寒羊（XH）共91只绵羊中的变异位点，并筛选出品种间差异显著的单核苷酸多态性（single nucleotide polymorphism，SNP）位点。针对筛选出的位点，利用飞行质谱检测技术对滩羊、小尾寒羊、杜泊羊和杜滩寒三元杂交羊（DTH）共30只绵羊进行基因分型，并与肉质性状进行关联分析。【结果】试验筛选出的MSTN基因rs129059715位点在4个绵羊群体中共检测到3种基因型，分别为15个CC基因型、9个CA基因型及6个AA基因型，C为优势等位基因（除杜滩寒三元杂交羊外）。在杜泊羊、滩羊和杜滩寒三元杂交羊群体中均处于Hardy-Weinberg平衡状态（P>0.05），在小尾寒羊群体中处于Hardy-Weinberg不平衡状态（P<0.05）；在杜泊羊和小尾寒羊群体中均处于低度多态（PIC<0.25）；在滩羊和杜滩寒三元杂交羊群体中均处于中度多态（0.25<PIC<0.50）。关联分析发现，rs129059715位点CA基因型个体背膘厚和反亚油酸含量均极显著高于CC基因型（P<0.01），净肉率、肾脏重及脂肪、十一碳酸、十三碳酸和十五碳酸含量均显著高于CC基因型（P<0.05）；CC基因型个体肌纤维直径显著高于AA基因型（P<0.05），pH显著高于CA基因型（P<0.05）；其他性状各基因型间均无显著差异（P>0.05）。【结论】试验经液相捕获测序技术获得MSTN基因具有差异显著的rs129059715位点，其对绵羊的屠宰性能、肉品质、脂肪酸含量均有显著影响，rs129059715位点可作为产肉性能和肉品质的潜在候选标记。

关键词: 绵羊; MSTN基因; 多态性; 屠宰性能; 肉品质

Abstract: 【Objective】 The aim of this study was to investigate the polymorphism of myostatin (MSTN) gene and its effect on meat quality traits in sheep, in order to provide effective genetic molecular marker for the selection and breeding of meat performance in sheep.【Method】 The variation sites of MSTN gene in 91 sheep including Dupo sheep (D), Tan sheep (T) and Small-tailed Han sheep (XH) were obtained using liquid phase capture sequencing technology, and the single nucleotide polymorphism (SNP) with significant interspecific differences were screened.A total of 30 sheep (D, T, XH and Dupo×Tan×Small-tailed Han sheep (DTH)) were genotyped for the selected site using in-flight mass spectrometry to analyze its association with meat quality traits in sheep.【Result】 Three genotypes were detected for rs129059715 of MSTN gene in 4 sheep populations, including 15 CC genotypes, 9 CA genotypes and 6 AA genotypes, with C being the dominant allele (except for Dupo×Tan×Small-tailed Han sheep). rs129059715 was in Hardy-Weinberg equilibrium in Dupo, Tan and Dupo×Tan×Small-tailed Han sheep populations (P>0.05), and was in Hardy-Weinberg disequilibrium in Small-tailed Han sheep population (P<0.05).rs129059715 was low polymorphic in Dupo and Small-tailed Han sheep populations (PIC<0.25), and was moderately polymorphic in Tan and Dupo×Tan×Small-tailed Han sheep populations (0.25<PIC<0.50).Association analysis revealed that the backfat thickness and trans-linoleic acid content of CA genotype at rs129059715 were extremely significantly higher than that of CC genotype (P<0.01), the net meat percentage, kidney weight, the contents of fat, undecanoic acid, decanoic acid and decanoic acid of CA genotype were significantly higher than that of CC genotype (P<0.05).The muscle fibre diameter of CC genotype was significantly higher than that of AA genotype (P<0.05), pH of CC genotype was significantly higher than that of CA genotype (P<0.05).There were no significant differences with other traits among different genotypes (P>0.05).【Conclusion】 Through liquid phase capture sequencing technology, rs129059715 of MSTN gene with significant differences was obtained, which had significant effects on the slaughter performance, meat quality and fatty acid content of sheep.rs129059715 could be used as a potential candidate marker for meat production performance and meat quality.

Key words: sheep; MSTN gene; polymorphism; slaughter performance; meat quality

中图分类号:

S813.3

陶毛孩, 孟科, 荣轩, 强浩, 聂玮, 郭晨浩, 冯登侦. 绵羊MSTN基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2023, 50(7): 2766-2776.

TAO Maohai, MENG Ke, RONG Xuan, QIANG Hao, NIE Wei, GUO Chenhao, FENG Dengzhen. Polymorphism of MSTN Gene and Its Association Analysis with Meat Quality Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2766-2776.

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