北京黑猪LPL和NUDT3基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.03.023

摘要/Abstract

摘要： 【目的】研究脂蛋白脂酶(lipoprteinlipase,LPL)和Nudix水解酶3(Nudix hydrolase homolog 3,NUDT3)基因多态性与北京黑猪肌内脂肪(intramuscular fat,IMF)含量和背膘厚的关联性,从而在分子水平上指导北京黑猪的育种工作。【方法】利用347头北京黑猪背最长肌样本进行IMF含量和背膘厚数据测定,按照IMF含量进行高、低分组。根据猪NUDT3和LPL基因序列设计引物并进行PCR扩增及测序,运用DNAStar软件中SeqMan分析测序结果,使用Duncan’s多重检验对2个基因不同基因型与北京黑猪IMF含量和背膘厚进行关联分析,并运用实时荧光定量PCR对其进行基因表达差异分析。【结果】LPL基因存在1个错义突变、3个同义突变和3个3'-UTR突变;NUDT3基因存在6个3'-UTR突变。LPL基因CDS区c.431 G＞C位点与北京黑猪第6~7肋背膘厚显著相关,c.647 G＞C位点与胸腰结合处背膘厚显著相关,c.637 A＞G位点与第6~7肋背膘厚、胸腰结合处背膘厚和腰荐结合处背膘厚均显著相关(P＜0.05);3'-UTR区c.1683 A＞G位点与胸腰结合处背膘厚显著相关,c.1689 C＞A位点与IMF含量显著相关(P＜0.05)。NUDT3基因上的突变位点均发生在3'-UTR区,其中c.7204 G＞T位点与北京黑猪IMF含量和腰荐结合处背膘厚显著相关,c.6068 C＞G位点与IMF含量和第6~7肋背膘厚显著相关,c.5729 G＞A位点与IMF含量显著相关(P＜0.05)。实时荧光定量PCR分析发现,北京黑猪NUDT3基因c.7204 G＞T位点IMF含量高、低组间表达量无显著差异,LPL基因c.1689 C＞A位点CC与AA基因型间表达量无显著差异(P＞0.05)。【结论】北京黑猪群体中LPL和NUDT3基因的突变位点与IMF含量显著关联,为北京黑猪肉品质选育提供了新的分子标记。

关键词: 北京黑猪; NUDT3基因; LPL基因; 肉品质; 关联分析

Abstract: 【Objective】 This study was aimed to investigate the association between the polymorphism of lipoprotein lipase (LPL) and Nudix hydrolase 3 (NUDT3) genes with intramuscular fat (IMF) content and backfat thickness in Beijing Black pigs,in order to guide the breeding of Beijing Black pigs at the molecular level.【Method】 Using 347 samples of the longissimus dorsi muscle in Beijing Black pigs,the IMF content and back fat thickness data were measured,and the high and low IMF content was grouped.Primers were designed based on NUDT3 and LPL gene sequences of pigs,and PCR amplification and sequencing were performed.The sequencing results were analyzed using SeqMan in DNAStar softwares.Duncan’s multiple test statistical analysis was used to analyze the association between different genotypes of NUDT3 and LPL genes and IMF content and backfat thickness in Beijing Black pigs.The gene expression difference was analyzed using Real-time quantitative PCR.【Result】 There were 1 missense mutation,3 synonymous mutations and 3 3'-UTR mutations in LPL gene,and 6 3'-UTR mutations in NUDT3 gene.The c.431 G＞C of LPL gene CDS was significantly associated with 6-7 rib back fat thickness,c.647 G＞C was significantly associated with thoracolumbar junction back fat thickness,and c.637 A＞G was significantly correlated with 6-7 rib back fat thickness,at the thoracolumbar junction back fat thickness and lumbar sacral junction back fat thickness in Beijing Black pigs (P＜0.05).The c.1683 A＞G of LPL gene 3'-UTR region was significantly correlated with backfat thickness at the thoracolumbar junction;c.1689 C＞A was significantly correlated with IMF content in Beijing Black pigs (P＜0.05).The mutation sites of NUDT3 gene all occured in 3'-UTR region,c.7204 G＞T was significantly correlated with IMF content and back fat thickness at the lumbar sacral junction,c.6068 C＞G was significantly correlated with IMF content and 6-7 rib back fat thickness,c.5729 G＞A was significantly correlated with IMF content in Beijing Black pigs (P＜0.05).Real-time quantitative PCR analysis showed that there was no significant difference in the expression of NUDT3 gene c.7204 G＞T between the high and low IMF content groups,and there was also no significant difference in the expression of LPL gene c.1689 C＞A between CC and AA genotypes (P＞0.05).【Conclusion】 The mutation sites of LPL and NUDT3 genes were significantly associated with IMF content in Beijing Black pigs,providing new molecular markers for meat quality breeding in Beijing Black pigs.

Key words: Beijing Black pigs; NUDT3 gene; LPL gene; meat quality; correlation analysis

中图分类号:

S813.3

苏艳芳, 牛乃琪, 宗文成, 刘先策, 刘海, 石国华, 井西涛, 张龙超. 北京黑猪LPL和NUDT3基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2024, 51(3): 1111-1121.

SU Yanfang, NIU Naiqi, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Polymorphisms of LPL and NUDT3 Genes and Its Association Analysis with Meat Quality Traits in Beijing Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1111-1121.

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