›› 2012, Vol. 39 ›› Issue (5): 148-151.

• 遗传繁育 • 上一篇    下一篇

卵泡刺激素β基因编码区SNPs与糯谷猪繁殖性能的关联性分析

褚维伟1, 高颖2, 林家栋1, 闫雷1, 潘兰兵1, 李沛丽1   

  1. 1. 贵州大学动物科学学院, 高原山地动物遗传育种与繁殖省部共建教育部重点实验室, 贵州贵阳 550025;2. 贵州大学动物科学学院, 贵州省动物疫病研究室, 贵州贵阳 550025
  • 收稿日期:2011-11-23 修回日期:1900-01-01 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 林家栋

Association Analyses of Single Nucleotide Polymorphism of FSHβ Gene on Reproductive Performance in Nuogu Pig

CHU Wei-wei1, GAO Ying2, LIN Jia-dong1, YAN Lei1, PAN Lan-bing1, LI Pei-li1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China;2. Laboratory for Amimal Disease of Guizhou, College of Animal Science, Guizhou University, Guiyang 550025, China
  • Received:2011-11-23 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20

摘要: 本试验采用RCR-SSCP及测序的方法检测了58头糯谷猪母猪卵泡刺激素(follicle-stimulating hormone,FSH) β基因编码区3个外显子的多态性,并分析了不同基因型与繁殖性能的关联性,结果发现,在外显子1和外显子3中未发现多态位点,外显子2中发现了2个新的多态位点,共检测出3个等位基因,5个基因型,未检测到BB基因型。其中A等位基因在6699位点发生了C→T突变,B等位基因在6708位点发生了T→C突变,C等位基因与公布序列一致,A、B、C等位基因频率分别为0.288、0.119、0.593。2个突变均为同义突变,各基因型间的繁殖性能差异不显著。

关键词: 糯谷猪; FSHβ基因; 外显子; PCR-SSCP; SNPs

Abstract: This study aimed to investigate molecular genetic characteristics of the follicle-stimulating hormone (FSH) β gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on some reproductive performance in Nuogu pig. We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing the coding sequence of the FSHβ gene in 58 individuals. The results showed that two new polymorphic sites were found in exon 2 on 6699 bp and 6708 bp. The A allele frequency was 0.288,the B allele frequency was 0.119,while the C allele frequency was 0.593. There were no significant difference in five genotypes(AA,CC,AB,AC,BC),because of the two mutant sites were the synonymy mutations.

Key words: Nuogu pig; FSHβ gene; exon; PCR-SSCP; SNPs

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