柯乐猪PAEP基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.04.027

中国畜牧兽医 ›› 2024, Vol. 51 ›› Issue (4): 1593-1602.doi: 10.16431/j.cnki.1671-7236.2024.04.027

• 遗传繁育 • 上一篇

柯乐猪PAEP基因多态性及其与繁殖性状的关联分析

赵永¹, 郭小江³, 李维², 熊力⁴, 杨红文², 王春源¹, 吴燕¹, 向进¹, 张依裕¹

1. 贵州大学动物科学学院, 高原山地动物遗传育种与繁殖教育部重点实验室, 贵州省动物遗传育种与繁殖重点实验室, 贵州大学香猪研究所, 贵阳 550025;
2. 贵州省畜禽遗传资源管理站, 贵阳 550001;
3. 贵州省草地技术试验推广站, 贵阳 550001;
4. 贵州省动物疫病预防控制中心, 贵阳 550001

收稿日期:2023-09-27 发布日期:2024-03-27
通讯作者: 张依裕 E-mail:zyy8yyc@163.com
作者简介:赵永,E-mail:1877753477@qq.com。
基金资助:
贵州省科技计划项目(黔科合平台人才[2021]5630号);贵州省科技支撑计划(黔科合支撑[2021]一般147);贵州省生猪产业技术体系建设(GZSZCYJSTX-03);贵州省生猪产业发展项目(黔财农[2021]157号、黔财农[2022]183号);贵州省种业发展项目(黔农计财[2022]10号);国家重点研发计划子课题(2022YFD1100308-01)

Polymorphism of PAEP Gene and Its Association with Reproductive Traits in Kele Pigs

ZHAO Yong¹, GUO Xiaojiang³, LI Wei², XIONG Li⁴, YANG Hongwen², WANG Chunyuan¹, WU Yan¹, XIANG Jin¹, ZHANG Yiyu¹

1. Key Laboratory of Plateau and Mountain Animal Genetics and Breeding and Reproduction of Ministry of Education, Guizhou Provincial Key Laboratory of Animal Genetics and Breeding and Reproduction, Institute of Swine, College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Guizhou Station of Livestock and Poultry Genetic Resources Management, Guiyang 550001, China;
3. Guizhou Grassland Technology Experiment and Extension Station, Guiyang 550001, China;
4. Guizhou Center for Animal Disease Control and Prevention, Guiyang 550001, China

Received:2023-09-27 Published:2024-03-27

摘要/Abstract

摘要： 【目的】研究孕激素相关子宫内膜蛋白(progestogen associated endometrial protein,PAEP)基因多态性对柯乐猪繁殖性状的影响,以挖掘柯乐猪的高繁殖力基因,用于提升柯乐猪的繁殖力。【方法】以189头经产柯乐猪为试验动物,采用PCR产物测序和序列比对鉴定单核苷酸多态性(SNP)位点,利用SHEsis在线软件分析SNP位点的群体遗传特性,通过RNAfold、SOPMA、SWISS-MODEL和SWISS-PdbViewer程序对SNP位点进行生物信息学分析,利用SPSS 22.0软件分析PAEP基因SNP位点与柯乐猪繁殖性状的相关性。【结果】在柯乐猪PAEP基因中共鉴定到3个SNPs位点:外显子4的g.1884992 T＞C位点,为错义突变,导致第135位缬氨酸(V)变成丙氨酸(A);内含子4的g.1885152 G＞C和g.1887834 G＞A位点。3个突变位点均检测到3种基因型;g.1884992 T＞C位点属于低度多态(PIC＜0.25),g.1885152 G＞C、g.1887834 G＞A位点属于中度多态(0.25＜PIC＜0.5)。g.1884992 T＞C位点产生的基因型分布未偏离Hardy-Weinberg平衡(P＞0.05);g.1885152 G＞C和g.1887834 G＞A位点产生的基因型分布均极显著偏离Hardy-Weinberg平衡(P＜0.01)。连锁分析发现,g.1885152 G＞C和g.1887834 G＞A位点完全连锁,共产生3种单倍型和6种双倍型。g.1884992 T＞C位点引起PAEP基因mRNA及蛋白的二级结构发生改变。关联分析结果表明,g.1884992 T＞C位点对柯乐猪总产仔数和产活仔数的影响均达到显著水平(P＜0.05),g.1885152 G＞C、g.1887834 G＞A位点对柯乐猪总产仔数、产活仔数、断奶仔数和断奶窝重的影响均达到显著水平(P＜0.05);双倍型H3H3个体总产仔数、产活仔数、断奶仔数和断奶窝重均显著高于其他5种双倍型(P＜0.05),初生窝重显著高于双倍型H1H1、H2H2和H2H3(P＜0.05);双倍型H1H3个体的总产仔数和断奶仔数显著高于双倍型H1H1、H1H2、H2H2和H2H3(P＜0.05),产活仔数显著高于双倍型H2H3(P＜0.05)。【结论】PAEP基因中存在的3个SNPs对柯乐猪繁殖性状有显著影响,其中H3H3为有利双倍型,可作为柯乐猪繁殖性状选择的遗传标记。

关键词: 柯乐猪; PAEP基因; 单核苷酸多态性; 繁殖性状

Abstract: 【Objective】 The aim of this study was to investigate the influence of polymorphism in progestogen associated endometrial protein (PAEP) gene on reproductive traits in Kele pigs,with the objective of identifying genetic markers that could enhance their reproductive traits.【Method】 A total of 189 multiparous Kele pigs were used as experimental animals,the single nucleotide polymorphism (SNP) were identified in PAEP gene through PCR product sequencing and sequence alignment.The SHEsis online software was utilized to analyze the population genetic characteristics of SNP.Bioinformatics analysis of SNP was carried out using RNAfold,SOPMA,SWISS-MODEL and SWISS-PdbViewer programs.The SPSS 22.0 software was employed to analyze the correlation between SNP of PAEP gene and reproductive traits in Kele pigs.【Result】 Three SNP sites were identified in PAEP gene of Kele pigs,a missense mutation g.1884992 T＞C in exon 4,resulting in the change of valine (V) at position 135 to alanine (A),as well as mutations in intron 4:g.1885152 G＞C and g.1887834 G＞A.All three SNPs displayed three genotypes.g.1884992 T＞C was low polymorphism (PIC＜0.25),while g.1885152 G＞C and g.1887834 G＞A were moderate polymorphism (0.25＜PIC＜0.5).The genotype distribution of g.1884992 T＞C did not deviate from Hardy-Weinberg equilibrium (P＞0.05),while the genotype distribution of g.1885152 G＞C and g.1887834 G＞A were extremely significantly deviated from Hardy-Weinberg equilibrium (P＜0.01).Linkage analysis revealed that there was complete linkage between g.1885152 G＞C and g.1887834 G＞A,resulting in the generation of 3 haplotypes and 6 diplotypes.g.1884992 T＞C resulted in the changes of the secondary structure in mRNA and protein of PAEP gene.The association analysis showed that g.1884992 T＞C was significantly affected total litter size and total number born in Kele pigs (P＜0.05),while g.1885152 G＞C and g.1887834 G＞A were significantly affected total litter size,total number born,number of weaned and weaning litter weight in Kele pigs (P＜0.05).The total litter size,total number born,number of weaned and weaning litter weight of H3H3 diplotype in Kele pigs were significantly higher than those of other 5 diplotypes (P＜0.05),and the birth weight of H3H3 diplotype in Kele pigs was significantly higher than that of H1H1,H2H2 and H2H3 diplotypes (P＜0.05).The total number born and number of weaned of H1H3 diplotype were significantly higher than those of H1H1,H1H2,H2H2 and H2H3 diplotypes (P＜0.05),and the total number born of H1H3 diplotype in Kele pigs was significantly higher than that of H2H3 diplotype (P＜0.05).【Conclusion】 Three SNPs found in PAEP gene significantly impacted the reproductive traits in Kele pigs.H3H3 was advantageous diplotype,and might serve as a genetic marker for the selection of reproductive traits in Kele pigs.

Key words: Kele pigs; PAEP gene; SNP; reproductive traits

中图分类号:

S813.3

赵永, 郭小江, 李维, 熊力, 杨红文, 王春源, 吴燕, 向进, 张依裕. 柯乐猪PAEP基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2024, 51(4): 1593-1602.

ZHAO Yong, GUO Xiaojiang, LI Wei, XIONG Li, YANG Hongwen, WANG Chunyuan, WU Yan, XIANG Jin, ZHANG Yiyu. Polymorphism of PAEP Gene and Its Association with Reproductive Traits in Kele Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1593-1602.

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柯乐猪PAEP基因多态性及其与繁殖性状的关联分析

Polymorphism of PAEP Gene and Its Association with Reproductive Traits in Kele Pigs

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