›› 2011, Vol. 38 ›› Issue (9): 122-125.

• 遗传繁育 • 上一篇    下一篇

牛组织蛋白D基因多态性与肉质、胴体性状的关联分析

邓桂馨, 许尚忠, 高雪, 李俊雅, 高会江, 袁峥嵘, 周正奎, 黄萌, 李姣   

  1. 中国农业科学院北京畜牧兽医研究所,北京 100193
  • 收稿日期:2011-02-16 修回日期:1900-01-01 出版日期:2011-09-20 发布日期:2011-09-20
  • 通讯作者: 许尚忠

Polymorphisms in CTSD Gene and Associations with Carcass and Meat Quality Traits in Cattle

DENG Gui-xin, XU Shang-zhong, GAO Xue, LI Jun-ya, GAO Hui-jiang, YUAN Zheng-rong, ZHOU Zheng-kui, HUANG Meng, LI Jiao   

  1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2011-02-16 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

摘要: 以51头西门塔尔和鲁西黄牛的杂交后代作为试验动物,扩增组织蛋白酶D(cathepsin D,CTSD)基因部分外显子,通过DNA测序方法检测到该基因第5、6、8外显子共4个SNPs(T581C、T599C、A861T、A1128G)的遗传变异。SAS软件采用最小二乘法拟合线性模型,将几个位点的不同基因型与牛肉质、胴体性状进行关联分析。结果表明,位于CTSD基因外显子5的T581C突变与大理石纹性状呈显著相关(P<0.05),BB基因型显著优于AA和AB基因型,这为T581C作为肉牛大理石纹的分子标记提供了一定的理论依据。

关键词: 牛; CTSD基因; 肉质性状; 胴体性状

Abstract: 4 exons of CTSD gene from 51 Simmental×Luxi cattles were amplified and sequenced. 4 SNPs were identified at position 581,599,861 and 1128 bp of CTSD gene(NM 001166521.1) by sequencing. The effects of 4 mutations on meat quality and carcass traits were analyzed by using general linear model. The results showed that T581C polymorphism significantly associated with marbling trait(P<0.05). Genotype of BB had better marbling grade than AA and AB genotypes. Thus T581C might be a good marker for marbling traits.

Key words: cattle; CTSD gene; meat quality traits; carcass traits

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