›› 2008, Vol. 1 ›› Issue (11): 74-76.

• 遗传繁育 • 上一篇    下一篇

季节和方法对牛、绵羊卵母细胞回收及体外成熟的影响

汪立芹,杨梅,陈童,郭志勤   

  1. 新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,乌鲁木齐 830000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-11-20 发布日期:2008-11-20

The Effects of Season, Collection Methods on the Recovery and Maturation of Oocytes in Cattle and Sheep

WANG Liqin, YANG Mei, CHEN Tong, GUO Zhiqin   

  1. Key Laboratory of Livestock Reproduction&Breed Biotechnology Ministry of Agriculture, Animal Science Academy of Xinjiang, Urumqi 830000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20

摘要: 利用屠宰牛、绵羊卵巢,研究了季节和方法对卵母细胞回收及体外成熟的影响。结果表明,季节对牛卵母细胞的回收和利用没有影响;抽吸法是合适的牛卵巢卵母细胞采集方法;季节对绵羊卵母细胞的回收和利用有显著影响,绵羊在非繁殖季节卵母细胞的回收率和可用率显著高于繁殖季节(P<0.05),而体外成熟率极显著低于繁殖季节(P<0.01);切割法回收的绵羊卵母细胞可用率(P<0.01)和体外成熟率(P<0.05)显著高于抽吸法,适合绵羊卵母细胞的回收。

关键词: 牛; 绵羊; 卵母细胞; 季节; 采集方法; 回收率

Abstract: The study collected cattle and sheep ovaries from slaughter house to search the effects of season, collection methods on the recovery and maturation. The results were as follows: the season have no effect on the recovery and maturation of oocytes in bovine; pumping method was suitable to bovine. Season has significant effects on the recovery and maturation of sheep. The number of oocytes recovered and utilized significant reduce from unreproduction season to reproduction season(P<0.05).However, the maturation rate in vitro increased extremely(P<0.01).Dissect way is suit to collect oocytes of sheep. The collection method have significant effect on availability rate(P<0.01)and maturation rate (P<0.05)between dissect way and aspiration.

Key words: cattle; sheep; oocyte; season; collection method; recovery

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